hnRNP C1 + C2 Antibody (CL2593) - Azide and BSA Free

Staining in U2OS cell line with Anti-hnRNP C1 + C2 monoclonal antibody) staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).

Staining in U251 cell line with Anti-hnRNP C1 + C2 monoclonal antibody) staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).

Lane 1: Marker [kDa]Lane 2:Human cell line U-251 MG

Staining in MCF7 cell line with Anti-hnRNP C1 + C2 monoclonal antibody) staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).

Staining in HeLa cell line with Anti-hnRNP C1 + C2 monoclonal antibody) staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).

Staining in A431 cell line with Anti-hnRNP C1 + C2 monoclonal antibody) staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).

Staining of human fallopian tube shows strong nuclear immunoreactivity.

Staining of human kidney shows strong nuclear immunoreactivity.

Staining of human prostate shows strong nuclear positivity in glandular and connective tissue cells.

Staining of human rectum shows strong nuclear immunoreactivity in glandular and lamina propria cells.

Staining of human small intestine shows strong nuclear positivity in glandular epithelium and in lamina propria cells.

Staining of human testis shows nuclear positivity in seminiferous tubules.

Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-hnRNP C1 + C2 antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clone: CL2593
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

hnRNP C1 + C2 Antibody (CL2593) - Azide and BSA Free Summary

• Immunogen: This antibody was generated using a recombinant protein sequence of P07910, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: HNRNPC
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry-Paraffin 1:1000 - 1:2500
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. For Immunocytochemistry/ Immunofluorescence/IF, Fixation/Permeabilization with PFA/Triton X-100 is recommended.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Alternate Names for hnRNP C1 + C2 Antibody (CL2593) - Azide and BSA Free

• C1
• C2
• heterogeneous nuclear ribonucleoprotein C (C1/C2)
• heterogeneous nuclear ribonucleoproteins C1/C2
• hnRNP C1/C2
• HNRNP
• hnRNPC
• HNRPC
• MGC104306
• MGC105117
• MGC117353
• MGC131677
• nuclear ribonucleoprotein particle C1 protein
• nuclear ribonucleoprotein particle C2 protein
• SNRPC

Background

RNA polymerase II transcripts in the nucleus are in complex with several proteins called heterogeneous nuclear ribonucleoproteins (hnRNPs). These proteins are important in biological activities such as transcription, pre-mRNA processing, cytoplasmic mRNA translation, and turnover. hnRNPs can be isolated either by immunoprecipitation or by sucrose gradient fractionation of cell extracts. When this is performed, the hnRNPs are isolated (consisting of protein groups named A to U), and many of these protein groups consist of more than one isoform. The major steadystate proteins of the isolated hnRNP complex are the A1, A2, B1, B2, C1, and C2 with a range of molecular weight starting with 34 kDa up to 43 kDa. The hnRNP-C proteins have a single RNP motif RNA-binding domain (RBD) of 80 to 100 amino acid long. The hnRNP -C proteins preferentially bind to uridine-rich RNA sequences. Oligomerization of the protein through leucine rich regions in its C-terminal end is important for RNA binding.4 Although its physiological action is unknown, mutation of the hnRNPC gene causes a embryonic lethal phenotype.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HNRNPC