HNF1 Antibody

Product Discontinued

HNF1 Antibody Summary

• Immunogen: Peptide with sequence C-DELPTKKGRRNRFK corresponding to internal region according to NP_000536.3.
• Epitope: DELPTKKGRRNRFK
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: HNF1A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:4000
• Application Notes: WB: Preliminary experiments in human liver and kidney lysates gave no specific signal but low background.
• Control:

  HNF1 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Notes

Please note, this antibody is considered Innovators Grade. Innovators Grade antibodies are generally unvalidated and require additional characterization for most new species/applications. Novus has made these antibodies available through our Innovators Reward program. Complete an online review with image, detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Please contact us at innovators@novusbio.com for more details.

Alternate Names for HNF1 Antibody

• HNF1 homeobox A
• IDDM20
• LFB1transcription factor 1, hepatic; LF-B1, hepatic nuclear factor (HNF1), albuminproximal factor
• MODY3
• TCF1hepatic
• Transcription factor 1

Background

TCF 1 (T cell factor 1) is a member of the TCF/LEF (lymphoid enhancer factor) family of transcription factors. Members of this family along with 226; catenin, are involved in mediating Wnt signaling by transactivating downstream genes. TCF 1 is closely related to LEF-1, both of which are transcription factors expressed in mice during T cell differentiation. They regulate the T cell receptor 225; enhancer in transfections assays. TCF 1 becomes transcriptionally active through interaction with 226; catenin. Also, numerous splice variants of TCF 1 have been identified. Overexpression of TCF-1-specific splice forms appears to correlate with the metastatic behavior of colorectal cancer cells and with overproduction of lymphoid tyrosine protein kinase p56(lck).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HNF1 Antibody (NB100-55425)(1)

Publication using NB100-55425

• Hansen SK, Parrizas M, Jensen ML et al. Genetic evidence that HNF-1alpha-dependent transcriptional control of HNF-4alpha is essential for human pancreatic beta cell function. J Clin Invest 2002-09-01 [PMID: 12235114]

Bioinformatics

• Gene Symbol: HNF1A
• Entrez:
  • 6927(Human)
  • 21405(Mouse)
  • 24817(Rat)
• Uniprot:
  • NP_000536.3(Human)