HNF-3 beta/FoxA2 Antibody

Western Blot: HNF-3 beta/FoxA2 Antibody [NB100-1263] - (1ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: HNF-3 beta/FoxA2 Antibody [NB100-1263] - (3.8ug/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Western Blot: HNF-3 beta/FoxA2 Antibody [NB100-1263] - (1ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western Blot: HNF-3 beta/FoxA2 Antibody [NB100-1263] - (1ug/ml) staining of Rat Lung lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

HNF-3 beta/FoxA2 Antibody Summary

• Immunogen: Peptide with sequence C-GVYSRPIMNSS corresponding to C-Terminus according to NP_710141.1.
• Localization: Nuclear
• Specificity: This antibody is expected to recognise both reported isoforms.
• Predicted Species: Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: FOXA2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 4 - 8 ug/ml
  • Immunohistochemistry-Paraffin
  • Peptide ELISA Detection limit 1:4000
  • Western Blot 1-3 ug/ml
• Application Notes: WB: Approx. 60 kDa band observed in Human Liver and Rat Lung lysates (calculated MW of 48.3kDa according to Human NP_710141.1 and 48.5kDa according to Rat NP_036875.1). IHC-P: Human prostate shows nuclear staining in the secretory cells of the gland.
• Control:

  HNF-3 beta/FoxA2 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for HNF-3 beta/FoxA2 Antibody

• forkhead box A2
• Forkhead box protein A2
• FoxA2
• hepatic nuclear factor-3-beta
• hepatocyte nuclear factor 3, beta
• hepatocyte nuclear factor 3-beta
• HNF3 beta
• HNF-3 beta
• HNF-3B
• HNF-3-beta
• HNF3BTCF-3B
• MGC19807
• TCF3B
• Transcription factor 3B

Background

This gene encodes a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific transcripts such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver. This gene has been linked to sporatic cases of maturity-onset diabetes of the young. Two transcript variants encoding the same protein have been identified for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HNF-3 beta/FoxA2 Antibody (NB100-1263)(2)

Publications using NB100-1263

• Nakamura K, Moore R, Negishi M, Sueyoshi T. Nuclear pregnane X receptor cross-talk with FoxA2 to mediate drug-induced regulation of lipid metabolism in fasting mouse liver. J Biol Chem. 2007-03-30 [PMID: 17267396]
• Wang H, Gauthier BR, Hagenfeldt-Johansson KA et al. Foxa2 (HNF3beta ) controls multiple genes implicated in metabolism-secretion coupling of glucose-induced insulin release. J Biol Chem 2002-05-01 [PMID: 11875061]

Bioinformatics

• Gene Symbol: FOXA2
• Entrez:
  • 3170(Human)
  • 15376(Mouse)
  • 25099(Rat)
• Uniprot:
  • NP_068556(Human)