HNF-3 beta/FoxA2 Overexpression Lysate (Native)


Western Blot: FOXA2 Overexpression Lysate (Adult Normal) [NBL1-10799] - Left - Empty vector transfected control cell lysate (HEK293 cell lysate); Right - Over-expression Lysate for FOXA2.

Product Details

Reactivity HuSpecies Glossary
Applications WB

Order Details

HNF-3 beta/FoxA2 Overexpression Lysate (Native) Summary

The lysate was created in HEK293T cells, using plasmid ID RC211408 and based on accession number NM_153675.
C-terminal DDK tag
Homo sapiens forkhead box A2 (FOXA2), transcript variant 2, mRNA.


Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
48.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Store at -80C. Avoid freeze-thaw cycles.
RIPA buffer

Lysate Details for HNF-3 beta/FoxA2

Protein State


HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for HNF-3 beta/FoxA2 Overexpression Lysate (Native)

  • forkhead box A2
  • Forkhead box protein A2
  • FoxA2
  • hepatic nuclear factor-3-beta
  • hepatocyte nuclear factor 3, beta
  • hepatocyte nuclear factor 3-beta
  • HNF3 beta
  • HNF-3 beta
  • HNF-3B
  • HNF-3-beta
  • MGC19807
  • TCF3B
  • Transcription factor 3B


This gene encodes a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific genes such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver. This gene has been linked to sporadic cases of maturity-onset diabetes of the young. Transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq]. Transcript Variant: This variant (2) has a different splice pattern at the 5' end compared to transcript variant 1, resulting in translation initiation from a downstream AUG, and a shorter isoform (2) missing 6 aa from the N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript matching the reference genomic sequence was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P
Species: Hu
Applications: ICC/IF
Species: Hu, Mu, Po, Bv, Rb
Applications: WB, Flow, ICC/IF, IHC-Fr, IHC-P, IP, PAGE
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu, Ca, Pm
Applications: WB, IHC-P
Species: Hu
Applications: WB, ChIP, IHC, IHC-P
Species: Hu
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
Species: Hu
Applications: WB, Simple Western, IHC, ICC
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt
Applications: Flow, IHC-P, IF
Species: Hu
Applications: WB, Simple Western, ChIP, ICC
Species: Hu
Applications: WB, ELISA, Flow, IHC, IHC-P, CyTOF-ready, ICC, IF
Species: Hu
Applications: WB, ICC
Species: Hu
Applications: WB, IHC, IHC-P
Species: Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt, Pm
Applications: WB, Flow, ICC/IF
Species: Hu
Applications: WB

Publications for HNF-3 beta/FoxA2 Lysate (NBL1-10799) (0)

There are no publications for HNF-3 beta/FoxA2 Lysate (NBL1-10799).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for HNF-3 beta/FoxA2 Lysate (NBL1-10799) (0)

There are no reviews for HNF-3 beta/FoxA2 Lysate (NBL1-10799). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for HNF-3 beta/FoxA2 Lysate (NBL1-10799) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional HNF-3 beta/FoxA2 Products

Bioinformatics Tool for HNF-3 beta/FoxA2 Lysate (NBL1-10799)

Discover related pathways, diseases and genes to HNF-3 beta/FoxA2 Lysate (NBL1-10799). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for HNF-3 beta/FoxA2 Lysate (NBL1-10799)

Discover more about diseases related to HNF-3 beta/FoxA2 Lysate (NBL1-10799).

Pathways for HNF-3 beta/FoxA2 Lysate (NBL1-10799)

View related products by pathway.

PTMs for HNF-3 beta/FoxA2 Lysate (NBL1-10799)

Learn more about PTMs related to HNF-3 beta/FoxA2 Lysate (NBL1-10799).

Blogs on HNF-3 beta/FoxA2

There are no specific blogs for HNF-3 beta/FoxA2, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Albumin Antibody

Contact Information

Product PDFs


Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.


Review this Product

Be the first to review our HNF-3 beta/FoxA2 Overexpression Lysate (Native) and receive a gift card or discount.


Gene Symbol FOXA2