HNF-3 alpha/FoxA1 Antibody

Product Discontinued

HNF-3 alpha/FoxA1 Antibody Summary

• Immunogen: A synthetic peptide derived from near C-terminus of human FOXA1 protein.
• Localization: Cytoplasm, Nucleus
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: FOXA1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
• Application Notes: For IHC-Paraffin: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min. Antigen retrieval can be performed using a Citrate process.

Reactivity Notes

Human. Predicted cross-reactivity based on sequence identity: Dog, Mouse, Rat, Pig, Fungi

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: 7.0 ml prediluted antibody with 10mM PBS (pH 7.4) and 1.0% BSA
• Preservative: 0.1% Sodium Azide
• Purity: Immunogen affinity purified

Notes

For a positive control use Breast carcinoma.

Alternate Names for HNF-3 alpha/FoxA1 Antibody

• forkhead box A1
• Forkhead box protein A1
• FoxA1
• hepatocyte nuclear factor 3, alpha
• hepatocyte nuclear factor 3-alpha
• HNF3 alpha
• HNF-3 alpha
• HNF-3A
• HNF-3-alpha
• HNF3ATCF-3A
• MGC33105
• TCF3A
• Transcription factor 3A

Background

FOXA1 is a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific transcripts such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HNF-3 alpha/FoxA1 Antibody (NBP1-49791)(1)

Publication using NBP1-49791

• Brunner, A L, Li, J Et al. A shared transcriptional program in early breast neoplasias despite genetic and clinical distinctions. Genome Biol 2014-05-23 [PMID: 24887547] (IF/IHC, Human)

Bioinformatics

• Gene Symbol: FOXA1
• Entrez:
  • 3169(Human)
• Uniprot:
  • P55317()