HMGN1/HMG14 Antibody

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Western Blot: HMG14 Antibody [NBP2-14842] - Analysis of HMG14 in Daudi lysate in the 1) absence and 2) presence of immunizing peptide probed with 2 ug/ml of HMG14 antibody. Goat anti-rabbit Ig HRP secondary antibody and ...read more

Product Details

Summary
Reactivity Hu, Po, Bv, Ca, Pm, RbSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

HMGN1/HMG14 Antibody Summary

Immunogen
A portion of amino acids 1-50 from human HMG14 was used as the immunogen for this antibody.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HMGN1
Purity
Protein A purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 2 ug/ml

Reactivity Notes

Reacts with Cow, Dog, Human, New World monkey, Pig, Rabbit, Rhesus Monkey and White-cheeked gibbon. Predicted to react with Mouse, Elephant, Chimpanzee, Chicken, Guinea Pig, Mole Rat, Opossum and Carolina anole. The HMG14 amino acid sequence used as immunogen for this antibody is 100% homologous in human, cow, new world monkey, dog, rhesus monkey, white-cheeked gibbon, rabbit, and pig, 94% in guinea pig, mole rat, elephant, chimpanzee, and orangutan, 83% in panda, opossum, mouse, and rat, 78% in zebra finch, 72% in carolina anole, and wild turkey, and 61% homologouus in platypus.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS containing 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein A purified

Alternate Names for HMGN1/HMG14 Antibody

  • FLJ31471
  • High mobility group nucleosome-binding domain-containing protein 1
  • high-mobility group (nonhistone chromosomal) protein 14
  • high-mobility group nucleosome binding 1
  • high-mobility group nucleosome binding domain 1
  • HMG14
  • HMG14FLJ27265
  • HMGN1
  • MGC104230
  • MGC117425
  • non-histone chromosomal protein HMG-14
  • nonhistone chromosomal protein HMG-14

Background

HMG14, also known as HMGN1, along with HMG17 and HMGN2 are non-histone proteins that bind nucleosomal cores and nucleosomal DNA (1, 2). Like transcription regulatory factors, they may travel to and from the nucleus or at least dissociate and bind again to DNA promoting the folding and unfolding of DNA regulatory sequences exposed to or hiding from binding sites of regulatory factors (3). Both HMG14 and HMG17 affect remodeling and therefore regulate gene activation or repression through transcription factor activity or DNA repair mechanisms (4, 5). Antibody to HMG14 aids in identifying its role in epigenetic related studies.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol HMGN1
Entrez
Uniprot