HMGB3/HMG4 Antibody (S01-9C9)

Western Blot: HMGB3/HMG4 Antibody (S01-9C9) [NBP3-15041] - Western blot detection of HMGB3/HMG4 in K562, C6, 3T3, Hela cell lysates using NBP3-15041 (1:1000 diluted). Predicted band size: 23kDa. Observed band size: 23kDa.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: S01-9C9
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

HMGB3/HMG4 Antibody (S01-9C9) Summary

• Description: Novus Biologicals Rabbit HMGB3/HMG4 Antibody (S01-9C9) (NBP3-15041) is a recombinant monoclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthetic peptide of human HMGB3/HMG4 (Uniprot#: O15347)
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: HMGB3
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1/2000-1/10000
• Theoretical MW: 23 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA
• Preservative: 0.01% Sodium Azide
• Purity: Affinity purified

Alternate Names for HMGB3/HMG4 Antibody (S01-9C9)

• High mobility group protein 2a
• High mobility group protein 4
• high mobility group protein B3
• high-mobility group (nonhistone chromosomal) protein 4
• high-mobility group box 3
• HMG2A
• HMG-2a
• HMG2AHMG-4
• HMG4
• HMG4MGC90319
• HMGB3
• non-histone chromosomal protein

Background

HMGB3 belongs to the high mobility group (HMG) protein superfamily. Like HMG1 (MIM 163905) and HMG2 (MIM 163906), HMGB3 contains DNA-binding HMG box domains and is classified into the HMG box subfamily. Members of the HMG box subfamily are thought to play a fundamental role in DNA replication, nucleosome assembly and transcription (Wilke et al., 1997 [PubMed 9370291]; Nemeth et al., 2006 [PubMed 16945912]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HMGB3