Recombinant Human HMGB1/HMG-1 Protein

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SDS-Page: Recombinant Human HMGB1/HMG-1 Protein [NBC1-18488] - HMGB1, 25kDa (223aa), confirmed by MALDI-TOF with a purity of 90% by SDS - PAGE.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
1 mg/ml

Order Details

Recombinant Human HMGB1/HMG-1 Protein Summary

Description
A recombinant protein with a C-Terminal His-tag and corresponding to the amino acids 1-215 of Human HMGB1/HMG-1

Source: E.coli

Amino Acid Sequence: MGKGDPKKPR GKMSSYAFFV QTCREEHKKK HPDASVNFSE FSKKCSERWK TMSAKEKGKF EDMAKADKAR YEREMKTYIP PKGETKKKFK DPNAPKRPPS AFFLFCSEYR PKIKGEHPGL SIGDVAKKLG EMWNNTAADD KQPYEKKAAK LKEKYEKDIA AYRAKGKPDA AKKGVVKAEK SKKKKEEEED EEDEEDEEEE EDEEDEDEEE DDDDELEHHH HHH

Protein/Peptide Type
Recombinant Protein
Gene
HMGB1
Purity
>90%, by SDS-PAGE
Endotoxin Note
< 1.0 EU per 1 microgram of protein (determined by LAL method)

Applications/Dilutions

Theoretical MW
25 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20mM Tris pH 8.0, 1mM EDTA, 0.5mM DTT, 10% glycerol
Preservative
No Preservative
Concentration
1 mg/ml
Purity
>90%, by SDS-PAGE

Alternate Names for Recombinant Human HMGB1/HMG-1 Protein

  • Amphoterin
  • high mobility group box 1
  • High mobility group protein 1
  • high mobility group protein B1
  • high-mobility group (nonhistone chromosomal) protein 1
  • high-mobility group box 1
  • HMG1
  • HMG-1
  • HMG1DKFZp686A04236
  • HMG3
  • HMGB1
  • SBP-1
  • Sulfoglucuronyl carbohydrate binding protein

Background

High mobility group box1 protein (HMGB1) is a very abundant chromatin-binding protein residing in the eukaryotic cell nucleus and acting in the assembly of nucleoprotein complexes. Inside the cell, HMGB1 binds to DNA and has a role in transcriptional regulation. Outside the cell, HMGB1 acts as a cytokine and has activities that resemble those of tumor necrosis factor. Recombinant human HMGB1 was expressed in High Five insect cells and purified by conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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FAQs for HMGB1/HMG-1 Recombinant Protein (NBC1-18488). (Showing 1 - 2 of 2 FAQ).

  1. I am looking for the recombinant human HMGB-1 which we would like to use for cultured human intervertebral disc cells in vitro. 1)Is your HMGB-1 protein NBC1-18488 already sterilized? 2)Are there some articles in which NBC1-18488 is used for human cultured cells in vitro?
    • NBC1-18488 is not sterilized, and may not be suitable for use in live cells. We have only approved this protein to work for ELISA and western blot, and have not assessed its biological activity. As such, we do not have any references that cite this product in cell culture.
  2. Are both of these proteins any carrier protein free? HMG1L10 Protein (Catalog # P3789) and HMGB1 Protein (catalog # NBC1-18488)
    • These proteins are carrier free. Catalog # P3789 is supplied in 50 mM HEPES, 100mM NaCl, pH 7.5 (30% glycerol, 30 mM Glutathione, 0.5% TritonX-100, 1 mM dithiothreitol). Catalog #NBC1-18488 is supplied in liquid in 20mM Tris pH 8.0, 1mM EDTA, 0.5mM DTT, 10% glycerol.

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Bioinformatics

Gene Symbol HMGB1
Entrez