Human HMGB1/HMG-1 ELISA Kit (Colorimetric)

ELISA: Human HMGB1/HMG-1 ELISA Kit (Colorimetric) [NBP2-62766] - Samples were spiked with high concentrations of Human HMGB1/HMG-1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Human HMGB1/HMG-1 ELISA Kit (Colorimetric) [NBP2-62766] - Standard Curve Reference

HMGB1 concentrations for untreated cells compared to four treatments, showing a decrease in secreted HMGB1 with all treatments. Image from a verified customer review.

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 31.25 - 2000 pg/mL
• Sensitivity: 18.75 pg/mL

Human HMGB1/HMG-1 ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Human HMGB-1 in samples. No significant cross-reactivity or interference between Human HMGB-1 and analogues was observed.
• Standard Curve Range: 31.25 - 2000 pg/mL
• Sensitivity: 18.75 pg/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: %CV < 4.52
• Intra-Assay: %CV < 5.09
• Spike Recovery: 93 - 108%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: HMGB1

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human HMGB1/HMG-1 ELISA Kit (Colorimetric)

• Amphoterin
• high mobility group box 1
• High mobility group protein 1
• high mobility group protein B1
• high-mobility group (nonhistone chromosomal) protein 1
• high-mobility group box 1
• HMG1
• HMG-1
• HMG1DKFZp686A04236
• HMG3
• HMGB1
• SBP-1
• Sulfoglucuronyl carbohydrate binding protein

Background

HMGB1 and HMGB2 are part of the chromatin non-histone high mobility group proteins 1 and 2. HMGB1 and HMGB2 (containing multiple HMG-boxes) are conserved domains of 80 amino acids which mediate the DNA binding of many proteins. HMG box domains recognize DNA structure. Both HMGB1 and HMGB2 contain a N-terminal HMG box, a central HMG box, and an acidic carboxy terminus. The acidic tails of HMGB1 and HMGB2 contain multiple serine residues which match the phosphorylation consensus sites of casein kinase II, and phosphorylation of this domain appears to be important for proper functioning of these proteins. HMGB1 and HMGB2 have been shown to facilitate the binding of various sequence-specific transcription factors to their respective DNA binding sites. HMGB1 and HMGB2 may also serve as architectural factors that recognize and mediate DNA structural changes that accompany various events such as DNA repair, transcription, and replication.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for HMGB1/HMG-1 ELISA Kit (NBP2-62766)(39)

Publications using NBP2-62766

• Fan X, Song JW, Wang SY, Cao WJ Changes of Damage Associated Molecular Patterns in COVID-19 Patients Infectious Diseases & Immunity 2021-01-01
• Fernando Rodríguez de Fonseca, Francisco Medina-Paz, Mira Sapozhnikov, Isaac Hurtado-Guerrero, Leticia Rubio, Stella Martín-de-las-Heras, Nerea Requena-Ocaña, María Flores-López, María del Mar Fernández-Arjona, Patricia Rivera, Antonia Serrano, Pedro Serrano, Sara C. Zapico, Juan Suárez, Youssef Sari Plasma Concentrations of High Mobility Group Box 1 Proteins and Soluble Receptors for Advanced Glycation End-Products Are Relevant Biomarkers of Cognitive Impairment in Alcohol Use Disorder: A Pilot Study Toxics 2024-02-29 [PMID: 38535924]
• Vasiljevic T, Zapletal E, Tarle M et al. Targeting DAMPs by Aspirin Inhibits Head and Neck Cancer Stem Cells and Stimulates Radio-Sensitization to Proton Therapy. Cancers 2025-06-26 [PMID: 40647457]
• Ramcharran H, Ghosh A, Meng Q et Al. Meconium Influences Pulmonary Short-Chain Fatty Acid Concentration in Porcine Meconium Aspiration Model Biomed Hub 2024-12-04 [PMID: 39816637] (ELISA)
• Yang, X;Chen, H;Shen, W;Chen, Y;Lin, Z;Zhuo, J;Wang, S;Yang, M;Li, H;He, C;Zhang, X;Hu, Z;Lian, Z;Yang, M;Wang, R;Li, C;Pan, B;Xu, L;Chen, J;Wei, X;Wei, Q;Xie, H;Zheng, S;Lu, D;Xu, X; FGF21 modulates immunometabolic homeostasis via the ALOX15/15-HETE axis in early liver graft injury Nature communications 2024-10-03 [PMID: 39362839]
• �zbay Kurt FG, Cicortas BA, Balzasch BM et al. S100A9 and HMGB1 orchestrate MDSC-mediated immunosuppression in melanoma through TLR4 signaling Journal for Immunotherapy of Cancer 2024-01-01 [PMID: 39266214]
• Chang NP, DaPrano EM, Lindman M et Al. Neuronal DAMPs exacerbate neurodegeneration via astrocytic RIPK3 signaling JCI Insight 2024-05-07 [PMID: 38713518] (ELISA)
• Norina Tang, Tatsuo Kido, Jian Shi, Erin McCafferty, Judith M. Ford, Kaitlyn Dal Bon, Lynn Pulliam, Thomas Ichim Blood Markers Show Neural Consequences of LongCOVID-19 Cells 2024-03-08 [PMID: 38534322]
• Rubas, NC;Peres, R;Kunihiro, BP;Allan, NP;Phankitnirundorn, K;Wells, RK;McCraken, TA;Lee, RH;Umeda, L;Conching, A;Juarez, R;Maunakea, AK; HMGB1 mediates microbiome-immune axis dysregulation underlying reduced neutralization capacity in obesity-related post-acute sequelae of SARS-CoV-2 Scientific reports 2024-01-03 [PMID: 38172612]
• Thais F.C. Fraga-Silva, Ualter G. Cipriano, Marcilio J. Fumagalli, Giseli F. Correa, Carlos A. Fuzo, Douglas dos-Santos, Fabiola L.A.C. Mestriner, Christiane Becari, Andrea Teixeira-Carvalho, Jordana Coelho-dos-Reis, Mayra G. Menegueti, Luiz T.M. Figueiredo, Larissa Dias Cunha, Olindo A. Martins-Filho, Marcelo Dias-Baruffi, Maria Auxiliadora-Martins, Rita C. Tostes, Vania L.D. Bonato Airway epithelial cells and macrophages trigger IL-6-CD95/CD95L axis and mediate initial immunopathology of COVID-19 iScience 2023-10-31 [PMID: 38047070]
• Ghosh P, Sasaki K, Pulido Ruiz IA et al. Inflammatory macrophage to hepatocyte signals can be prevented by extracellular vesicle reprogramming Journal of cell science 2023-04-13 [PMID: 37051862] (ELISA, Human)
• MilAo JFF Utilizing cytotoxic lymphocytes for indirect shock-and-kill strategy in HIV-1 treatment Thesis Jul 13 1905 (ELISA)
• Furtado Milao JFI Utilizing cytotoxic lymphocytes for indirect shock-and-kill strategy in HIV-1 treatment Thesis 1905-07-13 (ELISA)
• Yao Tong, Yinga Wu, Jing Ma, Masataka Ikeda, Tomomi Ide, Courtney T. Griffin, Xi-Qin Ding, Shusheng Wang Comparative mechanistic study of RPE cell death induced by different oxidative stresses Redox Biology 2023-08-06 [PMID: 37566944] (ELISA, Human)
• Chang N, DaPrano E, Evans W et al. Neuronal DAMPs exacerbate neurodegeneration via astrocytic RIPK3 signaling bioRxiv 2023-07-24 [PMID: 37546744] (ELISA)
• Ruskowski K, Neb H, Talbot S Et al. Plasma RIPK3 And HMGB1 Predict Severe COVID-19 Progression In ICU Patients: A Single-Center Cohort Study Research Square 2021-11-19
• Palacios Y, Ramón-Luing LA, Ruiz A et al. COVID-19 patients with high TNF/IFN-? levels show hallmarks of PANoptosis, an inflammatory cell death Microbes and infection 2023-06-30 [PMID: 37394112] (S-ELISA)
  
  Details:
  Plasma Samples
• Hall DA, Voigt RM, Cantu-Jungles TM et al. An open label, non-randomized study assessing a prebiotic fiber intervention in a small cohort of Parkinson's disease participants Nature communications 2023-02-18 [PMID: 36801916] (ELISA)
• Sunil V, Mozhi A, Zhan W et al. In-situ vaccination using dual responsive organelle targeted nanoreactors Biomaterials 2022-11-01 [PMID: 36228516] (ELISA)
• Sunil V, Teoh JH, Mohan BC et al. Bioengineered immunomodulatory organelle targeted nanozymes for photodynamic immunometabolic therapy Journal of controlled release : official journal of the Controlled Release Society 2022-08-22 [PMID: 35987351]
  
  Details:
  used to measure the Extracellular HMGB1 released in to the serum-free media from the U87 spheroids incubated with various treatment groups.
• Balsamo JA, Penton KE, Zhao Z et al. An immunogenic cell injury module for the single-cell multiplexed activity metabolomics platform to identify promising anti-cancer natural products The Journal of biological chemistry 2022-08-02 [PMID: 35931117] (ELISA, Human)
• Jang SB, Jin SM, Kim HS et al. DAMP-modulating nanoparticle for successful pancreatic islet and stem cell transplantation Biomaterials 2022-07-12 [PMID: 35849998] (ELISA)
• Jarosova J, Macinga P, Krupickova L et al. Impact of Endoluminal Radiofrequency Ablation on Immunity in Pancreatic Cancer and Cholangiocarcinoma Biomedicines 2022-06-06 [PMID: 35740353] (ELISA)
• Planes R, Pinilla M, Santoni K et al. Human NLRP1 is a sensor of pathogenic coronavirus 3CL proteases in lung epithelial cells Molecular cell 2022-05-16 [PMID: 35594856] (ELISA, Human)
• Ruskowski K, Neb H, Talbot SR et al. Persistently Elevated Plasma Levels of RIPK3, MLKL, HMGB1, and RIPK1 in COVID-19 ICU Patients American journal of respiratory cell and molecular biology 2022-04-06 [PMID: 35385375] (ELISA)
• Atanasova KR, Yilmaz O Inflammasome Activation in Gingival Epithelial Cells Methods in molecular biology (Clifton, N.J.) 2022-02-25 [PMID: 35212963] (ELISA)
• Vaes RDW, Reynders K, Sprooten J et al. Identification of Potential Prognostic and Predictive Immunological Biomarkers in Patients with Stage I and Stage III Non-Small Cell Lung Cancer (NSCLC): A Prospective Exploratory Study Cancers 2021-12-13 [PMID: 34944879]
• Chen R, Huang Y, Quan J et al. HMGB1 as a potential biomarker and therapeutic target for severe COVID-19 Heliyon 2020-12-01 [PMID: 33313438] (ELISA, Human)
• Malty R, Hilbe R, Ahn S et al. Paridiprubart inhibits TLR4-dependant NF-?B activation by multiple pathogens bioRxiv 2023-06-28 (ELISA)
• Gonzalez-Chapa JA, PeNa-Martinez VM, VIlchez-Cavazos JF Et al. Systemic and Local Cytokines Profile Determine Severity and Prognosis in Human Septic Arthritis: A Pilot Study Archives of medical research 2021-10-23 [PMID: 34702588] (ELISA)
• Wu K, Feng J, Xiu Y et al. V delta 2 T cell subsets, defined by PD-1 and TIM-3 expression, present varied cytokine responses in acute myeloid leukemia patients Int. Immunopharmacol. 2020-01-16 [PMID: 31955066] (ELISA, Human)
• Khan Z Remote Ischemic Conditioning as an Adjunct Intervention in Traumatic Brain Injury Thesis 2023-01-01 (ELISA)
• Li W Mediators of inflammation in the development of non-alcoholic steatohepatitis and fibrosis Thesis 2023-01-01 (ELISA)
• Shi H, Gandhi AA, Smith SA Et al. Endothelium-protective, histone-neutralizing properties of the polyanionic agent defibrotide. JCI Insight [PMID: 34264868] (ELISA)
• Vannier AGL, Wardwell B, Fomin V et al. Serum HMGB1 Associates with Liver Disease and Predicts Readmission and Mortality in Patients with Alcohol Use Disorder Alcohol (Fayetteville, N.Y.) 2021-06-09 [PMID: 34118353] (ELISA)
• Sun B, Tang N, Peluso M et al. Characterization and Biomarker Analyses of Post-COVID-19 Complications and Neurological Manifestations Cells 2021-02-13 [PMID: 33668514] (ELISA, Human)
• Wei J, Alfajaro MM, DeWeirdt PC et al. Genome-wide CRISPR Screens Reveal Host Factors Critical for SARS-CoV-2 Infection Cell 2020-10-20 [PMID: 33147444] (ELISA, Human)
• Cai X, Biswas I, Panicker SR et al. Activated protein C inhibits LPS-mediated acetylation and secretion of HMGB1 in endothelial cells J. Thromb. Haemost. 2019-03-13 [PMID: 30865333] (ELISA, Human)

Reviews for HMGB1/HMG-1 ELISA Kit (NBP2-62766) (2)

Average Rating: 4

(Based on 2 reviews)

reviewed by: Verified Customer 06/26/2025

Summary

• Sample Tested: Cell culture supernatant
• Lot: KL29BN283539

reviewed by: Xuanrong Chen 07/02/2022

Summary

• Sample Tested: Blood mononuclear cells (PBMCs)
• Lot: FQZ17THT4U

FAQs for HMGB1/HMG-1 ELISA Kit (NBP2-62766). (Showing 1 - 2 of 2 FAQs).

1. Can we use the standard material in this HMGB1/HMG-1 ELISA kit past the 6 month expiration date?
   • Our products do not have a set expiration date, but we guarantee the performance for 6 months if stored properly. If stored properly, the kit will most likely still work past the guarantee date.
2. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: HMGB1