HMGB1/HMG-1 Antibody (115603) [Unconjugated]

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, Jurkat human acute T cell leukemia cell line, and HEK293 human embryonic kidney cell line. PVDF membrane was probed with 0.05 µg/mL of Mouse Anti-Human HMGB1/HMG-1 Monoclonal Antibody (Catalog # MAB1690) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for HMGB1/HMG-1 at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

HepG2 human hepatocellular carcinoma cell line was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. HMGB1/HMG‑1/DNA complexes were immunoprecipitated using 5 μg Mouse Anti-Human HMGB1/HMG‑1 PE-conjugated Monoclonal Antibody (Catalog # MAB1690) or control antibody (Catalog # MAB004) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Mouse IgG Secondary Antibody (Catalog # BAF007). Immunocomplexes were captured using 50 μL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. The AFP promoter was detected by standard PCR.

HCT-116 human colorectal carcinoma cell line was stained with Mouse Anti-Human HMGB1/HMG-1 Monoclonal Antibody (Catalog # MAB1690, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

HMGB1/HMG-1 was detected in immersion fixed CCD-18Co human colonic fibroblast cell line using Mouse Anti-Human HMGB1/HMG-1 Monoclonal Antibody (Catalog # MAB1690) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

HMGB1/HMG‑1 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Mouse Anti-Human HMGB1/HMG‑1 Monoclonal Antibody (Catalog # MAB1690) at 5 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (brown; Catalog # VC001) and counterstained with hematoxylin (blue). Specific staining was localized to cell nuclei. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

The effect of corticosterone on high-mobility group box-1 (HMGB1) release in primary cultured cortical astrocytes. (A) Increasing concentrations of corticosterone induced HMGB1 release from astrocytes. Cells were treated with the indicated concentrations of corticosterone for 24 h, and the amount of HMGB1 in the media was analyzed by Western blotting. Representative blots are shown (HMGB1: 30 kDa). The data are expressed as the mean ± SEM. * p < 0.05, ** p < 0.01 vs. basal (concentration “0”) (Dunnett’s test; n = 7–15). (B) Astrocytes were treated with 1 μM corticosterone for the indicated periods of time and the amount of HMGB1 in the media was analyzed by Western blotting. Representative blots are shown (HMGB1: 30 kDa). The data are expressed as the mean ± SEM. * p < 0.05, ** p < 0.01 vs. basal (time “0”) (Dunnett’s test; n = 3–15). (C) Astrocytes were treated with 1 μM corticosterone for 24 h, and media concentrations of HMGB1 were analyzed by ELISA. The data are expressed as the mean ± SEM. * p < 0.05 vs. vehicle (t-test; n = 5). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32344830), licensed under a CC-BY license. Not internally tested by R&D Systems.

The effect of corticosterone on high-mobility group box-1 (HMGB1) release in primary cultured cortical astrocytes. (A) Increasing concentrations of corticosterone induced HMGB1 release from astrocytes. Cells were treated with the indicated concentrations of corticosterone for 24 h, and the amount of HMGB1 in the media was analyzed by Western blotting. Representative blots are shown (HMGB1: 30 kDa). The data are expressed as the mean ± SEM. * p < 0.05, ** p < 0.01 vs. basal (concentration “0”) (Dunnett’s test; n = 7–15). (B) Astrocytes were treated with 1 μM corticosterone for the indicated periods of time and the amount of HMGB1 in the media was analyzed by Western blotting. Representative blots are shown (HMGB1: 30 kDa). The data are expressed as the mean ± SEM. * p < 0.05, ** p < 0.01 vs. basal (time “0”) (Dunnett’s test; n = 3–15). (C) Astrocytes were treated with 1 μM corticosterone for 24 h, and media concentrations of HMGB1 were analyzed by ELISA. The data are expressed as the mean ± SEM. * p < 0.05 vs. vehicle (t-test; n = 5). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32344830), licensed under a CC-BY license. Not internally tested by R&D Systems.

RAGE and HMGB1 are expressed in myofibroblasts of endarterectomised chronic thromboembolic tissues of CTEPH patients.One representative patient is shown (12 out of 15 patients (80%) displayed analogous staining patterns. Photograph showing the macroscopic aspect of a representative PEA specimen (A). Scale bar: 6 cm. Immunohistochemical expression of RAGE (B, scale bar: 20 µm), HMGB1 (C), vimentin (D), alpha-smooth muscle actin (E) and CD34 (F) on adjacent tissue sections of the PEA specimen shown in (A). Scale bar: 40 µm. RAGE receptor for advanced glycation endproducts, HMGB1 high mobility group box 1, CTEPH chronic thromboembolic pulmonary hypertension, PEA pulmonary endarterectomy. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0106440), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Hu
• Applications: WB, IHC, ChIP, CyTOF-ready, Flow, ICC/IF
• Clone: 115603
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

HMGB1/HMG-1 Antibody (115603) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human HMGB1/HMG-1
  Gly2-Glu215 (predicted)
  Accession # P09429
• Specificity: Detects human HMGB1/HMG-1 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: HMGB1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP) 5 ug/5 x 10^6 cells
  • CyTOF-ready
  • Immunocytochemistry 8-25 ug/mL
  • Immunohistochemistry 5-25 ug/mL
  • Intracellular Staining by Flow Cytometry 2.5 ug/10^6 cells
  • Western Blot 0.05 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for HMGB1/HMG-1 Antibody (115603) [Unconjugated]

• Amphoterin
• high mobility group box 1
• High mobility group protein 1
• high mobility group protein B1
• high-mobility group (nonhistone chromosomal) protein 1
• high-mobility group box 1
• HMG1
• HMG-1
• HMG1DKFZp686A04236
• HMG3
• HMGB1
• SBP-1
• Sulfoglucuronyl carbohydrate binding protein

Background

Human High-mobility group box 1 protein (HMGB1), previously known as HMG-1 or amphoterin, is a member of the high mobility group box family of non-histone chromosomal proteins (1‑3). Human HMGB1 is expressed as a 30 kDa, 215 amino acid (aa) single chain polypeptide containing three domains: two N-terminal globular, 70 aa positively charged DNA-binding domains (HMG boxes A and B), and a negatively charged 30 aa C-terminal region that contains only Asp and Glu (4, 5). Residues 27‑43 and 178‑184 contain a NLS. Posttranslational modifications of the molecule have been reported, with acetylation occurring on as many as 17 lysine residues (6). HMGB1 is expressed at high levels in almost all cells (2, 4). It was originally discovered as a nuclear protein that could bend DNA. Such bending stabilizes nucleosome formation and regulates the expression of select genes upon recruitment by DNA binding proteins (1, 7, 8). It is now known that HMGB1 can also act extracellularly, both as an inflammatory mediator that promotes monocyte migration and cytokine secretion, and as a mediator of T cell-dendritic cell interaction (1, 4, 7, 9, 10). The cytokine activity of HBMG1 is restricted to the HMG B box, (3) while the A box is associated with the helix-loop-helix domain of transcription factors (11). HMBG1 is released in response to cell death and as a secretion product. Although HMBG-1 does not possess a classic signal sequence, it appears to be secreted as an acetylated form via secretory endolysosome exocytosis (6, 12). Once secreted, HMGB1 transduces cellular signals through its high affinity receptor, RAGE and, possibly, TLR2 and TLR4 (1, 3, 4). Human HMGB1 is 100% aa identical to canine HMGB1 and 99% aa identical to mouse, rat, bovine and porcine HMGB1, respectively.

1. Lotze, M.T. and K.J. Tracey (2005) Nat. Rev. Immunol. 5:331.
2. Yang, H. et al. (2005) J. Leukoc. Biol. 78:1.
3. Dumitriu, I.E. et al. (2005) Trends Immunol. 26:381.
4. Degryse, B. and M. de Virgilio (2003) FEBS Lett. 553:11.
5. Wen, L. et al. (1989) Nucleic Acids Res. 17:1197.
6. Bonaldi, T. et al. (2003) EMBO J. 22:5551.
7. Muller, S. et al. (2001) EMBO J. 20:4337.
8. Bustin, M. (1999) Mol. Cell. Biol. 19:5237.
9. Wang, H. et al. (1999) Science. 285:248.
10. Dimitriu, I.E. et al. (2005) J. Immunol. 174:7506.
11. Najima, Y. et al. (2005) J. Biol. Chem. 280:27523.
12. Gardella, S. et al. (2002) EMBO Rep. 3:995.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HMGB1/HMG-1 Antibody (MAB1690)(34)

Publications using MAB1690

• Presto P, Ji G, Ponomareva O et al. Hmgb1 Silencing in the Amygdala Inhibits Pain-Related Behaviors in a Rat Model of Neuropathic Pain International Journal of Molecular Sciences 2023-07-26 [PMID: 37569320]
  
  Details:
  Dilutions: 1:1000
• Kitaoka, S;Tomohiro, A;Ukeshima, S;Liu, K;Wake, H;Kimura, S;Yamamoto, Y;Nishibori, M;Furuyashiki, T; Repeated Social Defeat Stress Induces HMGB1 Nuclear Export in Prefrontal Neurons, Leading to Social Avoidance in Mice Cells 2023-07-05 [PMID: 37443823] (Immunohistochemistry, Mouse)
• Yan Yan Li, Ya Ping Feng, Li Liu, Jin Ke, Xing Long Inhibition of HMGB1 suppresses inflammation and catabolism in temporomandibular joint osteoarthritis <em>via</em> NF-kappa B signaling pathway European Journal of Histochemistry 6/21/2022 [PMID: 35726537]
• Jennifer Simpson, Kirsten M. Spann, Simon Phipps MLKL Regulates Rapid Cell Death-independent HMGB1 Release in RSV Infected Airway Epithelial Cells Frontiers in Cell and Developmental Biology 2022-05-31 [PMID: 35712662] (Immunocytochemistry, Human)
• M Chen, Y Zhou, M Xue, R Zhu, L Jing, L Lin, C He, Y Qin Anti-HMGB1 antibody is a potential characteristic autoantibody for Sj�gren&#039;s syndrome Scientific Reports, 2022-04-11;12(1):6020. 2022-04-11 [PMID: 35411013] (ELISA, Human)
• FF You, J Zhang, F Cheng, K Zou, XQ Zhang, JF Chen ATG 4B Serves a Crucial Role in RCE-4-Induced Inhibition of the Bcl-2-Beclin 1 Complex in Cervical Cancer Ca Ski Cells International Journal of Molecular Sciences, 2021-11-14;22(22):. 2021-11-14 [PMID: 34830185] (Immunoprecipitation, Human)
• K Hisaoka-Na, H Azuma, F Ishikawa, Y Nakamura, D Wang, K Liu, H Wake, M Nishibori, Y Nakata, N Morioka Corticosterone Induces HMGB1 Release in Primary Cultured Rat Cortical Astrocytes: Involvement of Pannexin-1 and P2X7 Receptor-Dependent Mechanisms Cells, 2020-04-25;9(5):. 2020-04-25 [PMID: 32344830] (Western Blot, Rat)
• S Begum, F Moreau, A Leon Coria, K Chadee Entamoeba histolytica stimulates the alarmin molecule HMGB1 from macrophages to amplify innate host defenses Mucosal Immunol, 2019-11-26;0(0):. 2019-11-26 [PMID: 31772322] (Western Blot, Human)
• Cai X, Biswas I, Panicker SR et al. Activated protein C inhibits LPS-mediated acetylation and secretion of HMGB1 in endothelial cells J. Thromb. Haemost. 3/13/2019 [PMID: 30865333]
• OBJ Corneth, F Schaper, F Luk, PS Asmawidjaj, AMC Mus, G Horst, P Heeringa, RW Hendriks, J Westra, E Lubberts Lack of IL-17 Receptor A signaling aggravates lymphoproliferation in C57BL/6 lpr mice Sci Rep, 2019-03-11;9(1):4032. 2019-03-11 [PMID: 30858513] (ELISA Standard, Human)
• Line H. Tangerås, Gabriela B. Silva, Guro S. Stødle, Lobke M. Gierman, Bente Skei, Karin Collett et al. Placental inflammation by HMGB1 activation of TLR4 at the syncytium Placenta 2018-12-01 [PMID: 30501882]
• Jessica E. Davies, Bonita H.R. Apta, Matthew T. Harper Cross-reactivity of anti-HMGB1 antibodies for HMGB2 Journal of Immunological Methods 2018-05-01 [PMID: 29453955]
• Dennis W. Simon, Rajesh K. Aneja, Henry Alexander, Michael J. Bell, Hülya Bayır, Patrick M. Kochanek et al. Minocycline Attenuates High Mobility Group Box 1 Translocation, Microglial Activation, and Thalamic Neurodegeneration after Traumatic Brain Injury in Post-Natal Day 17 Rats Journal of Neurotrauma 2018-01-01 [PMID: 28699371]
• Anti-high mobility group box-1 (HMGB1) antibody attenuates delayed cerebral vasospasm and brain injury after subarachnoid hemorrhage in rats Sci Rep, 2016-11-24;6(0):37755. 2016-11-24 [PMID: 27883038] (IHC-P, Rat)
• Regan JK, Kannan PS, Kemp MW et al. Damage-Associated Molecular Pattern and Fetal Membrane Vascular Injury and Collagen Disorganization in Lipopolysaccharide-Induced Intra-amniotic Inflammation in Fetal Sheep Reprod Sci. 7/7/2015 [PMID: 26156854]
  
  Details:
  HMG14 [p Ser20, p Ser24] antibody was used for IHC-P staining on formalin-fixed chorioamnion tissues of fetal sheep and the IHC assay implicated - paraffin sections, microwave-based antigen retrieval in citric acid buffer at pH 6.0, endogenous peroxidase blocking with methyl alcohol/hydrogen peroxide, blocking with 2% serum in PBS, use of primary at 1:50 dilution, detection with anti-rabbit secondary and Vectastain ABC peroxidase kit with staining enhancement using nickel-DAB followed by incubation with Tris-cobalt (Figure 2).
• Thomas D. Walko, Valentina Di Caro, Jon Piganelli, Timothy R. Billiar, Robert S. B. Clark, Rajesh K. Aneja Poly(ADP-Ribose) Polymerase 1-Sirtuin 1 Functional Interplay Regulates LPS-Mediated High Mobility Group Box 1 Secretion Molecular Medicine 2015-03-12 [PMID: 25517228]
• Moser B, Megerle A, Bekos C, Janik S, Szerafin T, Birner P, Schiefer A, Mildner M, Lang I, Skoro-Sajer N, Sadushi-Kolici R, Taghavi S, Klepetko W, Ankersmit H Local and systemic RAGE axis changes in pulmonary hypertension: CTEPH and iPAH. PLoS ONE, 2014-09-04;9(9):e106440. 2014-09-04 [PMID: 25188497] (IHC, Human)
• Paulo Emílio C. Leite, Katia G. Lima-Araújo, Guilherme R. França, Jussara Lagrota-Candido, Wilson C. Santos, Thereza Quirico-Santos Implant of Polymer Containing Pentacyclic Triterpenes from Eugenia punicifolia Inhibits Inflammation and Activates Skeletal Muscle Remodeling Archivum Immunologiae et Therapiae Experimentalis 2014-12-01 [PMID: 24830560]
• Moser B, Janik S, Schiefer A, Mullauer L, Bekos C, Scharrer A, Mildner M, Renyi-Vamos F, Klepetko W, Ankersmit H Expression of RAGE and HMGB1 in thymic epithelial tumors, thymic hyperplasia and regular thymic morphology. PLoS ONE, 2014-04-04;9(4):e94118. 2014-04-04 [PMID: 24705787] (IHC-P, Human)
• Zhou H, Ji X, Wu Y, Xuan J, Qi Z, Shen L, Lan L, Li Q, Yin Z, Li Z, Zhao Z A dual-role of Gu-4 in suppressing HMGB1 secretion and blocking HMGB1 pro-inflammatory activity during inflammation. PLoS ONE, 2014-03-06;9(3):e89634. 2014-03-06 [PMID: 24603876] (ICC, Mouse)
• Suwara M, Green N, Borthwick L, Mann J, Mayer-Barber K, Barron L, Corris P, Farrow S, Wynn T, Fisher A, Mann D IL-1alpha released from damaged epithelial cells is sufficient and essential to trigger inflammatory responses in human lung fibroblasts. Mucosal Immunol, 2013-10-30;7(3):684-93. 2013-10-30 [PMID: 24172847] (ELISA Development, Human)
• Liou T, Adler F, Keogh R, Li Y, Jensen J, Walsh W, Packer K, Clark T, Carveth H, Chen J, Rogers S, Lane C, Moore J, Sturrock A, Paine R, Cox D, Hoidal J Sputum biomarkers and the prediction of clinical outcomes in patients with cystic fibrosis. PLoS ONE, 2012-08-10;7(8):e42748. 2012-08-10 [PMID: 22916155] (ELISA Development, Human)
• Sperling T, Oldak M, Walch-Ruckheim B, Wickenhauser C, Doorbar J, Pfister H, Malejczyk M, Majewski S, Keates AC, Smola S Human Papillomavirus Type 8 Interferes with a Novel C/EBPβ-Mediated Mechanism of Keratinocyte CCL20 Chemokine Expression and Langerhans Cell Migration. PLoS Pathog., 2012-07-26;8(7):e1002833. 2012-07-26 [PMID: 22911498] (Western Blot, Human)
• Colombo B, Canevali P, Magnani O, Rossi E, Puppo F, Zocchi M, Poggi A Defective expression and function of the leukocyte associated Ig-like receptor 1 in B lymphocytes from systemic lupus erythematosus patients. PLoS ONE, 2012-02-15;7(2):e31903. 2012-02-15 [PMID: 22355402] (ICC, Human)
• Donnelly , O G, Errington-Mais , F, Steele , L, Hadac , E, Jennings , V, Scott , K, Peach , H, Phillips , R M, Bond , J, Pandha , H, Harrington , K, Vile , R, Russell , S, Selby , P, Melcher , A A Measles virus causes immunogenic cell death in human melanoma. Gene Ther, 2011-12-15;20(1):7-15. 2011-12-15 [PMID: 22170342] (Western Blot, Human)
• Irshad Ali, Stephanie Gruenloh, Ying Gao, Anne Clough, John R. Falck, Meetha Medhora et al. Protection by 20-5,14-HEDGE Against Surgically Induced Ischemia Reperfusion Lung Injury in Rats The Annals of Thoracic Surgery 2012-01-01 [PMID: 22115333]
• Vitali R, Stronati L, Negroni A Fecal HMGB1 is a novel marker of intestinal mucosal inflammation in pediatric inflammatory bowel disease. Am. J. Gastroenterol., 2011-07-26;106(11):2029-40. 2011-07-26 [PMID: 21788990] (Western Blot, Human)
• Zhou J, Huang JD, Poon VK, Chen DQ, Chan CC, Ng F, Guan XY, Watt RM, Lu L, Yuen KY, Zheng BJ Functional dissection of an IFN-alpha/beta receptor 1 promoter variant that confers higher risk to chronic hepatitis B virus infection. J. Hepatol., 2009-05-03;51(2):322-32. 2009-05-03 [PMID: 19501422] (Western Blot, Human)
• Dave SH, Tilstra JS, Matsuoka K, Li F, DeMarco RA, Beer-Stolz D, Sepulveda AR, Fink MP, Lotze MT, Plevy SE Ethyl pyruvate decreases HMGB1 release and ameliorates murine colitis. J. Leukoc. Biol., 2009-05-19;86(3):633-43. 2009-05-19 [PMID: 19454652] (ELISA Development, Mouse)
• Rowe SM, Jackson PL, Liu G, Hardison M, Livraghi A, Solomon GM, McQuaid DB, Noerager BD, Gaggar A, Clancy JP, O'Neal W, Sorscher EJ, Abraham E, Blalock JE Potential role of high-mobility group box 1 in cystic fibrosis airway disease. Am. J. Respir. Crit. Care Med., 2008-07-24;178(8):822-31. 2008-07-24 [PMID: 18658107] (Neutralization, Western Blot, Human)
• Aychek T, Miller K, Sagi-Assif O, Levy-Nissenbaum O, Israeli-Amit M, Pasmanik-Chor M, Jacob-Hirsch J, Amariglio N, Rechavi G, Witz IP E-selectin regulates gene expression in metastatic colorectal carcinoma cells and enhances HMGB1 release. Int. J. Cancer, 2008-10-15;123(8):1741-50. 2008-10-15 [PMID: 18324679] (ICC, Immunodepletion, Western Blot, Human)
• Rao DA, Tracey KJ, Pober JS IL-1alpha and IL-1beta are endogenous mediators linking cell injury to the adaptive alloimmune response. J. Immunol., 2007-11-15;179(10):6536-46. 2007-11-15 [PMID: 17982042] (Western Blot, Human)
• Seki E, De Minicis S, Osterreicher CH, Kluwe J, Osawa Y, Brenner DA, Schwabe RF TLR4 enhances TGF-beta signaling and hepatic fibrosis. Nat. Med., 2007-10-21;13(11):1324-32. 2007-10-21 [PMID: 17952090] (Western Blot, Mouse)
• Zetterstrom CK, Jiang W, Wahamaa H, Ostberg T, Aveberger AC, Schierbeck H, Lotze MT, Andersson U, Pisetsky DS, Erlandsson Harris H Pivotal advance: inhibition of HMGB1 nuclear translocation as a mechanism for the anti-rheumatic effects of gold sodium thiomalate. J. Leukoc. Biol., 2007-10-03;83(1):31-8. 2007-10-03 [PMID: 17913975] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: HMGB1
• Uniprot:
  • P09429()