HLTF Antibody - BSA Free

Western Blot: HLTF Antibody [NBP2-16857] - Sample (30 ug of whole cell lysate) A: Hela 7. 5% SDS PAGE gel, diluted at 1:5000.

Immunocytochemistry/Immunofluorescence: HLTF Antibody [NBP2-16857] - Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using antibody at 1:500 dilution.

Immunohistochemistry-Paraffin: HLTF Antibody [NBP2-16857] - Immunohistochemical analysis of paraffin-embedded MB157 xenograft, using antibody at 1:500 dilution.

Immunoprecipitation: HLTF Antibody [NBP2-16857] - HLTF antibody immunoprecipitates HLTF protein in IP experiments. IP Sample: HeLa whole cell lysate/extract. A. 40 ug HeLa whole cell lysate/extract. B. Control with 2 ug of preimmune rabbit IgG. C. Immunoprecipitation of HLTF protein by 2 ug of HLTF antibody. 7.5% SDS-PAGE. The immunoprecipitated HLTF protein was detected by HLTF antibody diluted at 1:1000.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

HLTF Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human HLTF. The exact sequence is proprietary.
• Localization: Nucleus
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HLTF
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
  • Western Blot 1:1000-1:10000
• Theoretical MW: 114 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for HLTF Antibody - BSA Free

• DNA-binding protein/plasminogen activator inhibitor 1 regulator
• EC 3.6.1
• EC 3.6.4.-
• EC 6.3.2.-
• helicase-like transcription factor
• HIP116ARING finger protein 80
• HIP116matrix associated, actin dependent regulator of chromatin
• RNF80SWI/SNF-related matrix-associated actin-dependent regulator of chromatinsubfamily A member 3
• subfamily a, member 3
• Sucrose nonfermenting protein 2-like 3
• ZBU1

Background

This gene encodes a member of the SWI/SNF family. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein contains a RING finger DNA binding motif. Two transcript variants encoding the same protein have been found for this gene. However, use of an alternative translation start site produces an isoform that is truncated at the N-terminus compared to the full-length protein. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: HLTF
• Uniprot:
  • Q14527(Human)