HLTF Antibody

Western Blot: HLTF Antibody [NB100-1041] - staining (0.5ug/ml) of HeLa lysate (RIPA buffer, 30ug total protein per lane). Detected by chemiluminescence.

Flow Cytometry: HLTF Antibody [NB100-1041] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

• Reactivity: Hu, Pm, Mu, Rt, Bv, Ca
• Applications: WB, ELISA, Flow
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

HLTF Antibody Summary

• Immunogen: Peptide with sequence C-KQAKINEIRTLIDL corresponding to C-Terminus according to NP_003062.2, NP_620636.1.
• Specificity: NP_003062.2 and NP_620636.2 are varients that represent the same protein.
• Predicted Species: Mouse (100%), Rat (100%), Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: HLTF
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10ug/ml
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 0.3-1ug/ml
• Application Notes: Western blot: Approx. 125kDa band observed in nuclear lysates of cell line HeLa and in preliminary testing of A431 nuclear cell lysate (calculated MW of 114kDa according to NP_003062.2). This molecular weight is routinely observed by other sources. Recommended concentration: 0.3-1ug/ml. Primary incubation 1 hour at room temperature.
• Theoretical MW: 114 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Jurkat Whole Cell Lysate
  HLTF Overexpression Lysate

Reactivity Notes

Primate reactivity reported in scientific literature (PMID: 23415230). Predicted cross-reactivity based on sequence identity: Mouse, Rat, Canine, Bovine.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for HLTF Antibody

• DNA-binding protein/plasminogen activator inhibitor 1 regulator
• EC 3.6.1
• EC 3.6.4.-
• EC 6.3.2.-
• helicase-like transcription factor
• HIP116ARING finger protein 80
• HIP116matrix associated, actin dependent regulator of chromatin
• RNF80SWI/SNF-related matrix-associated actin-dependent regulator of chromatinsubfamily A member 3
• subfamily a, member 3
• Sucrose nonfermenting protein 2-like 3
• ZBU1

Background

The HLTF (Helicase-like Transcription Factor; SMARCA 3) gene is a member of the family of genes that remove proteins that coil around DNA. HLTF proteins aid the stabilization and regulation of the production of proteins in the cell. Inactivation of the HTLF gene may contribute to the transformation of normal colon cells into cancer cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HLTF Antibody (NB100-1041)(2)

Publications using NB100-1041

• Oh YS, Gao P, Lee KW et al. SMARCA3, a chromatin-remodeling factor, is required for p11-dependent antidepressant action. Cell 2013-02-14 [PMID: 23415230] (WB, ICC/IF, Human, Primate)
• Sheridan PL, Schorpp M, Voz ML, Jones KA. Cloning of an SNF2/SWI2-related protein that binds specifically to the SPH motifs of the SV40 enhancer and to the HIV-1 promoter. J Biol Chem 1995-03-03 [PMID: 7876228]

Bioinformatics

• Gene Symbol: HLTF
• Entrez:
  • 6596(Human)
  • 20585(Mouse)
• Uniprot:
  • Q14527(Human)