HLTF Overexpression Lysate (Native)

Images

 
Western Blot: HLTF Overexpression Lysate (Adult Normal) [NBL1-11599] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for HLTF.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

HLTF Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC207920 and based on accession number NM_003071.
Epitope
C-terminal Myc/DDK
Specificity
Homo sapiens helicase-like transcription factor (HLTF), transcript variant 1, mRNA.
Gene
HLTF

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
113.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for HLTF

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for HLTF Overexpression Lysate (Native)

  • DNA-binding protein/plasminogen activator inhibitor 1 regulator
  • EC 3.6.1
  • EC 3.6.4.-
  • EC 6.3.2.-
  • helicase-like transcription factor
  • HIP116ARING finger protein 80
  • HIP116matrix associated, actin dependent regulator of chromatin
  • RNF80SWI/SNF-related matrix-associated actin-dependent regulator of chromatinsubfamily A member 3
  • subfamily a, member 3
  • Sucrose nonfermenting protein 2-like 3
  • ZBU1

Background

This gene encodes a member of the SWI/SNF family. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein contains a RING finger DNA binding motif. Two transcript variants encoding the same protein have been found for this gene. However, use of an alternative translation start site produces an isoform that is truncated at the N-terminus compared to the full-length protein. [provided by RefSeq]. Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB100-57524
Species: Hu
Applications: WB, IHC, IHC-P, IP
NB100-2594
Species: Hu, Mu
Applications: WB, ChIP, IHC-P, IP, PLA
NBP1-90015
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NB200-106
Species: Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P
NB500-106
Species: Hu, Mu, Rt, Po, Ch, Dr, Fi, Pm, Rb, Ye, Ze
Applications: WB, Simple Western, ChIP, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, IHC-FrFl
NB100-56552
Species: Hu
Applications: WB
AF682
Species: Hu
Applications: WB, IHC, ELISA(Cap), ELISA(Det), Neut, ELISA(Sta)
NBP1-07100
Species: Hu, Mu
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
NB100-91662
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-19773
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P
AF3264
Species: Hu
Applications: WB, Simple Western, Flow, IHC, CyTOF-ready, ICC
AF4885
Species: Mu
Applications: WB, IP
MAB4218
Species: Hu
Applications: WB, IHC
NB100-168
Species: Hu, Mu(-)
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-P, CyTOF-ready, Flow-IC
NBP1-82454
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
AF2065
Species: Mu
Applications: WB, Simple Western, ICC
NBP1-87431
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-91776
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-71687
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P
NBL1-11599
Species: Hu
Applications: WB

Publications for HLTF Lysate (NBL1-11599) (0)

There are no publications for HLTF Lysate (NBL1-11599).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for HLTF Lysate (NBL1-11599) (0)

There are no reviews for HLTF Lysate (NBL1-11599). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for HLTF Lysate (NBL1-11599) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional HLTF Products

Bioinformatics Tool for HLTF Lysate (NBL1-11599)

Discover related pathways, diseases and genes to HLTF Lysate (NBL1-11599). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for HLTF Lysate (NBL1-11599)

Discover more about diseases related to HLTF Lysate (NBL1-11599).
 

Pathways for HLTF Lysate (NBL1-11599)

View related products by pathway.

PTMs for HLTF Lysate (NBL1-11599)

Learn more about PTMs related to HLTF Lysate (NBL1-11599).
 

Research Areas for HLTF Lysate (NBL1-11599)

Find related products by research area.

Blogs on HLTF

There are no specific blogs for HLTF, but you can read our latest blog posts.

Customers Who Bought This Also Bought

p19ARF Antibody
NB200-106
HLTF Antibody
NBP1-83256

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our HLTF Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol HLTF