HLTF Antibody (S03-1E4)

Western Blot: HLTF Antibody (S03-1E4) [NBP3-19653] - Western blot detection of HLTF in Hela cell lysates using NBP3-19653 (1:1000 diluted). Predicted band size: 114kDa. Observed band size: 114kDa.

Immunocytochemistry/Immunofluorescence: HLTF Antibody (S03-1E4) [NBP3-19653] - Immunofluorescence of HLTF (green) in Hela cells using NBP3-19653 at dilution 1/50, and DAPI (blue)

• Reactivity: Hu, Rt
• Applications: WB, ICC/IF
• Clone: S03-1E4
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.3 mg/ml

HLTF Antibody (S03-1E4) Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthetic peptide of human HLTF (Uniprot # Q14527)
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: HLTF
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1/20
  • Western Blot 1/2000-1/10000
• Theoretical MW: 114 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA
• Preservative: 0.01% Sodium Azide
• Concentration: 0.3 mg/ml
• Purity: Affinity purified

Alternate Names for HLTF Antibody (S03-1E4)

• DNA-binding protein/plasminogen activator inhibitor 1 regulator
• EC 3.6.1
• EC 3.6.4.-
• EC 6.3.2.-
• helicase-like transcription factor
• HIP116ARING finger protein 80
• HIP116matrix associated, actin dependent regulator of chromatin
• RNF80SWI/SNF-related matrix-associated actin-dependent regulator of chromatinsubfamily A member 3
• subfamily a, member 3
• Sucrose nonfermenting protein 2-like 3
• ZBU1

Background

Helicase-like Transcription Factor (HLTF) is a member of the the SWI/SNF family of nuclear enzymes that remove proteins that coil around DNA. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. HLTF proteins aid the stabilization and regulation of the production of proteins in the cell. Inactivation of the HTLF gene may contribute to the transformation of normal colon cells into cancer cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HLTF