HLA G Antibody (87G)

Images

 
Western Blot: HLA G Antibody (87G) [NBP1-43123] - Total protein from human and mouse placental tissue, mouse platelets and Ramos cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in ...read more
Immunocytochemistry/ Immunofluorescence: HLA G Antibody (87G) [NBP1-43123] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were ...read more
Flow Cytometry: HLA G Antibody (87G) [NBP1-43123] - Intracellular flow cytometric staining of 1 x 10^6 HEK-293 cells using HLA G antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ...read more
Flow Cytometry: HLA G Antibody (87G) [NBP1-43123] - Staining of stimulated U937 cells with 0.25 ug of Mouse IgG2a K Isotype Control Purified (blue) or 0.25 ug of Anti-Human HLA-G Purified (purple) followed by F(ab')2 ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, B/N, ELISA, Flow, ICC/IF, CyTOF-ready
Clone
87G
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

HLA G Antibody (87G) Summary

Immunogen
721.221 cells transfected with full length HLA G cDNA with high surface HLA G expression. [UniProt# P17693]
Specificity
Membrane bound HLA G1 and soluble HLA G5.
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
HLA-G
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/ml
  • Blocking/Neutralizing
  • ELISA
  • Flow Cytometry 1 ug per million cells
  • Immunocytochemistry/Immunofluorescence 1:100
  • CyTOF-ready
Application Notes
The HLA G Antibody (87G) has been reported to work for Immunocytochemistry/Immunofluorescence and for blocking the interaction of HLA G with inhibitory receptors (see publications).
Publications
Read Publications using
NBP1-43123 in the following applications:

Reactivity Notes

Human.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for HLA G Antibody (87G)

  • b2 microglobulin
  • HLA class I histocompatibility antigen, alpha chain G
  • HLA class I molecule
  • HLA G antigen
  • HLA G
  • HLA-6.0
  • HLA-G histocompatibility antigen, class I, G
  • HLAG
  • HLA-G
  • major histocompatibility complex, class I, G
  • MHC Class I Antigen G
  • MHC-G

Background

HLA histocompatibility antigen G, also known as human leukocyte antigen G (HLA G), is a protein that in humans is encoded by the HLA G gene. HLA G stems from a family of MHC class I molecules, which have an important function in immune response. In addition to helping present foreign antigens to the immune system, HLA G also aids in maternal tolerance of the fetus during pregnancy. Structurally, HLA G is a heterodimer consisting of a heavy and light chain, where the heavy chain anchors itself to the membrane. On a subcellular level, HLA G localizes to the plasma membrane. In tissue, HLA G is only expressed in trophoblasts.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

H00003126-M01
Species: Hu
Applications: WB, ELISA, IHC-P
NB120-6405
Species: Rt
Applications: B/N, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready, Flow-IC
NBP2-29931
Species: Hu
Applications: WB, Flow, IHC-P
NB100-64775
Species: Hu, Bb, Bv, Pm
Applications: ELISA, Flow, Func, ICC/IF, IHC, IHC-Fr, IP
AF-386-PB
Species: Hu
Applications: WB, Flow, CyTOF-ready, ICC
H00003117-M01
Species: Hu
Applications: WB, ELISA, PLA
NB100-524
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP, Flow-IC
MAB20172
Species: Hu
Applications: WB, CyTOF-reported, Neut
NBP2-50419
Species: Hu
Applications: Flow, IP, CyTOF-ready
NBP1-49045
Species: Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Cell Depl, CyTOF-ready, InhibTFunc
NBP1-88899
Species: Hu
Applications: IHC, IHC-P
NBP1-19371
Species: Hu, Mu, Rt, Ca, Rb
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
MAB2078
Species: Hu
Applications: WB, Flow, CyTOF-ready, Neut
MAB1058
Species: Hu
Applications: Flow, Block, CyTOF-ready
NBP1-92684
Species: Hu, Mu, Rt, Po, Bv
Applications: WB, ICC/IF

Publications for HLA G Antibody (NBP1-43123)(7)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 4 applications: B/N, ELISA, FLOW, ICC/IF.


Filter By Application
B/N
(2)
ELISA
(1)
FLOW
(5)
ICC/IF
(4)
All Applications
Filter By Species
Human
(7)
All Species
Showing Publications 1 - 7 of 7.
Publications using NBP1-43123 Applications Species
Lin A, Yan WH, Xu HH et al. HLA-G expression in human ovarian carcinoma counteracts NK cell function. Ann Oncol. 2007 Nov [PMID: 17846022] (FLOW, B/N, Human) FLOW, B/N Human
Contini P, Ghio M, Poggi A et al. Soluble HLA-A,-B,-C and -G molecules induce apoptosis in T and NK CD8+ cells and inhibit cytotoxic T cell activity through CD8 ligation. Eur J Immunol. 2003 Jan [PMID: 12594841] (B/N, ELISA, Human) B/N, ELISA Human
Hurks HM, Valter MM, Wilson L et al. Uveal melanoma: no expression of HLA-G. Invest Ophthalmol Vis Sci. 2001 Dec [PMID: 11726606] (FLOW, ICC/IF, Human) FLOW, ICC/IF Human
Lila N, Rouas-Freiss N, Dausset J et al. Soluble HLA-G protein secreted by allo-specific CD4+ T cells suppresses the allo-proliferative response: a CD4+ T cell regulatory mechanism. Proc Natl Acad Sci U S A. 2001 Oct 9 [PMID: 11572934] (FLOW, ICC/IF, Human) FLOW, ICC/IF Human
Le Gal FA, Riteau B, Sedlik C et al. HLA-G-mediated inhibition of antigen-specific cytotoxic T lymphocytes. Int Immunol. 1999 Aug [PMID: 10421792] (FLOW, Human) FLOW Human
Moreau P, Adrian-Cabestre F, Menier C et al. IL-10 selectively induces HLA-G expression in human trophoblasts and monocytes. Int Immunol. 1999 May [PMID: 10330285] (FLOW, ICC/IF, Human) FLOW, ICC/IF Human
Paul P, Cabestre FA, Le Gal FA et al. Heterogeneity of HLA-G gene transcription and protein expression in malignant melanoma biopsies. Cancer Res. 1999 Apr 15 [PMID: 10213506] (ICC/IF, Human) ICC/IF Human

Reviews for HLA G Antibody (NBP1-43123) (0)

There are no reviews for HLA G Antibody (NBP1-43123). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for HLA G Antibody (NBP1-43123) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-43123AF405
Alexa Fluor 488 Labeled NBP1-43123AF488
Alexa Fluor 647 Labeled NBP1-43123AF647
Alexa Fluor 700 Labeled NBP1-43123AF700
Allophycocyanin Labeled NBP1-43123APC
Biotin Labeled NBP1-43123B
DyLight 350 Labeled NBP1-43123UV
DyLight 405 Labeled NBP1-43123V
DyLight 488 Labeled NBP1-43123G
DyLight 550 Labeled NBP1-43123R
DyLight 650 Labeled NBP1-43123C
DyLight 680 Labeled NBP1-43123FR
DyLight 755 Labeled NBP1-43123IR
HRP Labeled NBP1-43123H
PE Labeled NBP1-43123PE
PerCP Labeled NBP1-43123PCP

Additional HLA G Products

Bioinformatics Tool for HLA G Antibody (NBP1-43123)

Discover related pathways, diseases and genes to HLA G Antibody (NBP1-43123). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for HLA G Antibody (NBP1-43123)

Discover more about diseases related to HLA G Antibody (NBP1-43123).
 

Pathways for HLA G Antibody (NBP1-43123)

View related products by pathway.

PTMs for HLA G Antibody (NBP1-43123)

Learn more about PTMs related to HLA G Antibody (NBP1-43123).
 

Research Areas for HLA G Antibody (NBP1-43123)

Find related products by research area.

Blogs on HLA G.

HLA G - mediating immune tolerance during pregnancy
Human leukocyte antigen G (HLA G) is a major histocompatibility complex (MHC) class I molecule that is primarily expressed in the placenta and is essential for the immune tolerance of the fetus during pregnancy. Unlike many HLA genes, HLA G has re...  Read full blog post.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our HLA G Antibody (87G) and receive a gift card or discount.

Bioinformatics

Gene Symbol HLA-G
Entrez
Uniprot