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HLA G Antibody (87G) - BSA Free

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Flow Cytometry: HLA G Antibody (87G) [NBP1-43123] - An intracellular stain was performed on Hek293 cells with HLA G Antibody [87G] NBP1-43123AF488 (blue) and a matched isotype control (orange). Both antibodies were ...read more
Immunocytochemistry/ Immunofluorescence: HLA G Antibody (87G) [NBP1-43123] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were ...read more
Flow Cytometry: HLA G Antibody (87G) [NBP1-43123] - An intracellular stain was performed on HeLa cells with HLA G [87G] Antibody NBP1-43123AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% ...read more
Flow Cytometry: HLA G Antibody (87G) [NBP1-43123] - Staining of stimulated U937 cells with 0.25 ug of Mouse IgG2a K Isotype Control Purified (blue) or 0.25 ug of Anti-Human HLA-G Purified (purple) followed by F(ab')2 ...read more
Flow Cytometry: HLA G Antibody (87G) [NBP1-43123] - Intracellular flow cytometric staining of 1 x 10^6 HEK-293 cells using HLA G antibody (dark blue) and isotype control (orange). An antibody concentration of 1 ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Simple Western, ELISA, Flow, Flow-CS, ICC/IF, IHC, IHC-Fr, B/N, CyTOF-ready
Clone
87G
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Order Details

HLA G Antibody (87G) - BSA Free Summary

Immunogen
721.221 cells transfected with full length HLA G cDNA with high surface HLA G Antibody (87G) expression. [UniProt# P17693]
Specificity
Membrane bound HLA G1 and soluble HLA G5.
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
HLA-G
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Block/Neutralize
  • CyTOF-ready
  • ELISA
  • Flow (Cell Surface)
  • Flow Cytometry 1 ug per million cells
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Simple Western 1:50
Application Notes
Use in Flow, IHC reported in scientific literature (PMID:34201301). Simple Western reported by an internal validation. Separated by Size, antibody dilution of 1:50, detects a band at 81 kDa.
Theoretical MW
39 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NBP1-43123 in the following applications:

  • B/N
    2 publications
  • 1 publication
  • 4 publications
  • 2 publications
  • 4 publications
  • IHC
    2 publications
  • WB
    1 publication

Reactivity Notes

Use in Human reported in scientific literature (PMID:34201301).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for HLA G Antibody (87G) - BSA Free

  • b2 microglobulin
  • HLA class I histocompatibility antigen, alpha chain G
  • HLA class I molecule
  • HLA G antigen
  • HLA G
  • HLA-6.0
  • HLA-G histocompatibility antigen, class I, G
  • HLAG
  • HLA-G
  • major histocompatibility complex, class I, G
  • MHC Class I Antigen G
  • MHC class Ib antigen
  • MHC-G
  • mutant MHC class I antigen
  • mutant MHC class Ib antigen
  • sHLA-G
  • Soluble HLA class I histocompatibility antigen, alpha chain G

Background

Human leukocyte antigen (HLA)-G is a non-classical major histocompatibility complex (MHC) class I gene belonging to the HLA class I family. The HLA class I family is subdivided into the classical (HLA-Ia) antigens comprised of HLA-A, -B, and -C molecules and the nonclassical (HLA-Ib) group including the HLA-E, -F, and -G molecules. Seven different splice variants of HLA-G have been observed including four membrane isoforms (HLA-G1, HLA-G2, HLA-G3, and HLA-G4) and three soluble isoforms (HLA-G5, HLA-G6 and HLA-G7). Proteolytic processing of HLA-G1 by metalloproteinases (MMPs) such as MMP-2 produces the soluble isoform, shedding HLA-G1. The HLA-G1 and HLA-G5 isoforms share a similar confirmation to the classical MHC class I protein, consisting of three extracellular domains including alpha1, alpha2, and alpha3 associated with Beta-2-microglobulin (B2M). Other HLA-G variants are shorter, missing one or two of the globular domains and are not bound to B2M. HLA-G receptors include KIR2DL4 (CD158d), with the highest affinity for ILT2 (LILRB1, CD85j) and ILT4 (LILRB2, CD85d) (1,2).

Discovered in cytotrophoblasts, HLA-G is involved in fetal maternal immune tolerance and some studies have linked its downregulation with severe preeclampsia (3). HLA-G mediated immune suppression works by impeding cell proliferation, differentiation, cytotoxicity, cytokine secretion and chemotaxis; and activation of regulatory cells and MDSCs or M2 type macrophage. HLA-G is constitutively expressed in immune-privileged sites such as pancreatic islets, mesenchymal stem cells (MSCs) and the cornea. Upregulation of HLA-G occurs in pathological states including cancer, allo-transplantations, viral infections, autoimmune and inflammatory diseases (4). In cancer, HLA-G expression is induced by hypoxia and has been correlated with advanced tumor stage, tumor metastasis, and poor therapeutic response and survival. HLA-G is an attractive tumor associated-antigen (TAA) for immunotherapy and is considered a major immune checkpoint (ICP).

References

1. Loustau, M., Anna, F., Drean, R., Lecomte, M., Langlade-Demoyen, P., & Caumartin, J. (2020). HLA-G Neo-Expression on Tumors. Front Immunol, 11:1685. PMID: 32922387

2. Lin A, Yan WH. (2018) Heterogeneity of HLA-G Expression in Cancers: Facing the Challenges. Front Immunol. 9:2164. PMID: 30319626

3. Djurisic S, Hviid TV. (2014) HLA Class Ib Molecules and Immune Cells in Pregnancy and Preeclampsia. Front Immunol. 5:652. PMID: 25566263

4. Lila N, Rouas-Freiss N, Dausset J, Carpentier A, Carosella ED. (2001) Soluble HLA-G protein secreted by allo-specific CD4+ T cells suppresses the allo-proliferative response: a CD4+ T cell regulatory mechanism. Proc Natl Acad Sci U S A. 98(21):12150-12155. PMID: 11572934

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for HLA G Antibody (NBP1-43123)(11)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 7 applications: B/N, ELISA, FLOW, Flow, ICC/IF, IHC, WB.


Filter By Application
B/N
(2)
ELISA
(1)
FLOW
(4)
Flow
(2)
ICC/IF
(4)
IHC
(2)
WB
(1)
All Applications
Filter By Species
Human
(11)
All Species
Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using NBP1-43123 Applications Species
Levy S, Somasundaram L, Raj IX et al. dCas9 fusion to computer-designed PRC2 inhibitor reveals functional TATA box in distal promoter region Cell reports Mar 1 2022 [PMID: 35235780] (WB, ICC/IF, Human) WB, ICC/IF Human
Mestrallet G, Carosella ED, Martin MT et al. Immunosuppressive Properties of Epidermal Keratinocytes Differ According to Their Immaturity Status Frontiers in immunology Feb 11 2022 [PMID: 35222373] (Flow, Human) Flow Human
Mestrallet G, AuvrE F, Schenowitz C et al. Human Keratinocytes Inhibit CD4+ T-Cell Proliferation through TGFB1 Secretion and Surface Expression of HLA-G1 and PD-L1 Immune Checkpoints Cells Jun 8 2021 [PMID: 34201301] (Flow, IHC, Human) Flow, IHC Human
Dissanayake K, NOmm M, LAttekivi F et al. Individually cultured bovine embryos produce extracellular vesicles that have the potential to be used as non-invasive embryo quality markers Theriogenology Jun 1 2020 [PMID: 32259747] (Human) Human
Lin A, Yan WH, Xu HH et al. HLA-G expression in human ovarian carcinoma counteracts NK cell function. Ann Oncol. 2007 Nov [PMID: 17846022] (FLOW, B/N, Human) FLOW, B/N Human
Ishitani A, Sageshima N, Lee N et al. Protein expression and peptide binding suggest unique and interacting functional roles for HLA-E, F, and G in maternal-placental immune recognition. J Immunol. 2003 Aug 1 [PMID: 12874228] (IHC, Human) IHC Human
Contini P, Ghio M, Poggi A et al. Soluble HLA-A,-B,-C and -G molecules induce apoptosis in T and NK CD8+ cells and inhibit cytotoxic T cell activity through CD8 ligation. Eur J Immunol. 2003 Jan [PMID: 12594841] (B/N, ELISA, Human) B/N, ELISA Human
Lila N, Rouas-Freiss N, Dausset J et al. Soluble HLA-G protein secreted by allo-specific CD4+ T cells suppresses the allo-proliferative response: a CD4+ T cell regulatory mechanism. Proc Natl Acad Sci U S A. 2001 Oct 9 [PMID: 11572934] (FLOW, ICC/IF, Human) FLOW, ICC/IF Human
Le Gal FA, Riteau B, Sedlik C et al. HLA-G-mediated inhibition of antigen-specific cytotoxic T lymphocytes. Int Immunol. 1999 Aug [PMID: 10421792] (FLOW, Human) FLOW Human
Moreau P, Adrian-Cabestre F, Menier C et al. IL-10 selectively induces HLA-G expression in human trophoblasts and monocytes. Int Immunol. 1999 May [PMID: 10330285] (FLOW, ICC/IF, Human) FLOW, ICC/IF Human
Show All 11 Publications.

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Harnessing Natural Killer Cell Activity for Anti-Tumor Immunotherapy
By Victoria Osinski, PhDWhat’s “Natural” About Natural Killer (NK) Cells?For immunologists, the term cytotoxicity often conjures up images of an army of antigen specific CD8+ T cells deploying to ...  Read full blog post.

HLA G - mediating immune tolerance during pregnancy
Human leukocyte antigen G (HLA G) is a major histocompatibility complex (MHC) class I molecule that is primarily expressed in the placenta and is essential for the immune tolerance of the fetus during pregnancy. Unlike many HLA genes, HLA G has re...  Read full blog post.

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Bioinformatics

Gene Symbol HLA-G
Entrez
Uniprot