HLA-E Antibody (3D12HLA-E)

Images

 
Flow Cytometry: HLA-E Antibody [NBP1-43124] - Analysis using the PE conjugate of NBP1-43124. Staining of normal human peripheral blood cells with Anti-Human HLA-ABC FITC and Mouse IgG1 kappa Isotype Control PE (left) or ...read more
Flow Cytometry: HLA-E Antibody [NBP1-43124] - Staining of normal human peripheral blood cells with 0.5 micrograms of Mouse IgG1 k Isotype Control Purified (open histogram) or 0.5 micrograms of Anti-Human HLA-E Purified ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, IP
Clone
3D12HLA-E
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

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Catalog# & Conjugate Size Price

HLA-E Antibody (3D12HLA-E) Summary

Immunogen
The immunogen for this antibody was HLAE.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
HLA-E
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Flow Cytometry 1:10-1:1000
  • Immunoprecipitation 1:10-1:500
Application Notes
This 3D12 (HLA-E) antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS, pH 7.2
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A or G purified

Alternate Names for HLA-E Antibody (3D12HLA-E)

  • DKFZp686P19218
  • EA1.2
  • EA2.1
  • HLA class I histocompatibility antigen, alpha chain E
  • HLA class I histocompatibility antigen, E alpha chain
  • HLA-6.2
  • HLAE
  • lymphocyte antigen
  • major histocompatibility complex, class I, E
  • MHC class I antigen E
  • MHC HLA-E alpha-1
  • MHC HLA-E alpha-2.1
  • MHC
  • QA1

Background

The monoclonal antibody 3D12 (HLA-E) recognizes human HLA-E, a member of the Human Leukocyte Antigen family. HLA-E is a MHC Class IB member, which has a specialized role in NK cell recognition. Binding of a restricted set of peptides derived from other Class I leader sequences to the HLA-E and B2M (beta2 microglobulin) complex leads to interaction with NKG2A on NK cells. This binding protects the HLA-G-expressing cell from NK cell killing. HLA-E expression is ubiquitous.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

Isotype Controls

Additional HLA-E Products

HLA-E NBP1-43124

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Bioinformatics

Gene Symbol HLA-E