HLA-DR Antibodies
Human Leukocyte Antigen -DR isotype (HLA-DR) is a major histocompatibility complex (MHC) class II molecule expressed by antigen presenting cells that functions in immune system response. HLA-DR molecules are heterodimers consisting of a 34 kDa alpha chain subunit and an approximately 30 kDa beta chain subunit. Our catalog includes a 5-star reviewed antibody featured in 29+ publications. Browse our extensive inventory for recombinant monoclonal antibodies, monoclonal antibodies, and HLA-DR-conjugated antibodies. Validated research applications for our HLA-DR-antibodies include CyTOF-ready, ELISA, Flow Cytometry, ICC, Immunofluorescence, IHC-Frozen, IHC-Paraffin, Immunoprecipitation, Radioimmunoassay, and Western Blot. Our HLA-DR antibodies are reactive in Human, Canine, Primate, and more. Each HLA-DR antibody is fully covered by Novus’ Guarantee+. Choose the anti-HLA-DR antibody best suited for your research needs by viewing the technical details, images, references, reviews, and related products.
Alternate Names for HLA-DR Antibodies
anti-HLA-DR antibody, anti-HLA-DRA antibody, anti-FLJ51114 antibody, anti-histocompatibility antigen HLA-DR alpha antibody, anti-HLA class II histocompatibility antigen, DR alpha chain antibody, anti-HLA-DRA1 antibody, anti-major histocompatibility complex, class II, DR alpha antibody, anti-MHC cell surface glycoprotein antibody, anti-MHC class II antigen DRA antibody, anti-MLRW antibody
20 Results for "hla-dr" in Primary Antibodies
DR555MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder.​" alt="HLA-DRA Antibody was detected in immersion fixed paraffin-embedded sections of human Tonsil using Mouse Anti-Human HLA-DRA Monoclonal Antibody (Catalog # MAB11555) at 5ug/mL at 32° Celsius for2 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 555 Goat anti-Mouse IgG Secondary Antibody at 1:100 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR555MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder.​" class="big_thumb" />
![Dual RNAscope ISH-IHC: HLA-DR Antibody (L243) [NB100-77855] - Formalin-fixed paraffin-embedded tissue sections of human metastatic tonsil were probed for HLA-DR mRNA (ACD RNAScope probe, catalog # 475891; Fast Red chromogen, ACD catalog # 322500). Adjacent tissue section was processes for immunohistochemistry using mouse monoclonal (Novus catalog # NB100-77855) at 1:50 dilution for 1 hour at room temperature followed by incubation with the anti-mouse IgG VisUCyte HRP Polymer Antibody (Catalog # VC001) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue).](http://images.novusbio.com/fullsize/HLA-DR-Antibody-L243-Dual-RNAscope-ISH-IHC-NB100-77855-img0016.jpg "<b>Orthogonal Strategies Validation. </b>Dual RNAscope ISH-IHC: HLA-DR Antibody (L243) [NB100-77855] - Formalin-fixed paraffin-embedded tissue sections of human metastatic tonsil were probed for HLA-DR mRNA (ACD RNAScope probe, catalog # 475891; Fast Red chromogen, ACD catalog # 322500). Adjacent tissue section was processes for immunohistochemistry using mouse monoclonal (Novus catalog # NB100-77855) at 1:50 dilution for 1 hour at room temperature followed by incubation with the anti-mouse IgG VisUCyte HRP Polymer Antibody (Catalog # VC001) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue).")
![<b>Orthogonal Strategies Validation. </b>Western Blot: HLA-DR Antibody (L243) [NB100-77855] - Analysis of human spleen (A) and human tonsil (B) tissue using HLA-DR antibody at 2 ug/mL.](http://images.novusbio.com/fullsize/HLA-DR-Antibody-L243-Western-Blot-NB100-77855-img0002.jpg)
![Immunohistochemistry-Paraffin: HLA-DR Antibody (L243) [NB100-77855] - Staining of HLA-DR in human spleen using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/HLA-DR-Antibody-L243-Immunohistochemistry-Paraffin-NB100-77855-img0003.jpg)
![Flow Cytometry: HLA-DR Antibody (L243) [NB100-77855] - Analysis using the FITC conjugate of NB100-77855. Staining of Human peripheral blood lymphocytes with L243 FITC.](http://images.novusbio.com/fullsize/HLA-DR-Antibody-L243-Flow-Cytometry-NB100-77855-img0015.jpg)
![Flow Cytometry: HLA-DR Antibody (L243) [NB100-77855] - Human peripheral blood lymphocytes stained with L243 PE](http://images.novusbio.com/fullsize/HLA-DR-Antibody-L243-Flow-Cytometry-NB100-77855-img0001.jpg)
![Flow (Cell Surface): HLA-DR Antibody (L243) [NB100-77855] - Analysis using the Azide Free version of NB100-77855. Staining of HLA-DR expression by 5x10^5 BDCM cells using 0.5 ug of (red) and 0.5 ug of isotype control (green). This antibody and an anti-mouse IgG PE conjugated secondary antibody were used.](http://images.novusbio.com/fullsize/HLA-DR-Antibody-L243-Flow-Cell-Surface-NB100-77855-img0010.jpg)
![Flow Cytometry: HLA-DR Antibody (L243) [NB100-77855] - Analysis using the PerCP/Cy5.5 conjugate of NB100-77855. Staining of HLA-DR expression by 5x10^5 human PBMC using 10 uL (0.25 ug) of this antibody. Cell surface flow kit was used for this test. Left panel shows the monocyte gate, right panel shows the lymphocyte gate.](http://images.novusbio.com/fullsize/HLA-DR-Antibody-L243-Flow-Cytometry-NB100-77855-img0011.jpg)
![Flow Cytometry: HLA-DR Antibody (L243) [NB100-77855] - Analysis using the Alexa Fluor (R) 700 conjugate of NB100-77855. Staining of HLA-DR protein in human PBMCs using HLA-DR antibody [clone L243; conjugated to Alexa Fluor (R) 700] at 1:100 with 25 minutes incubation at 4C in 2% human serum, 0.5 mM EDTA in DPBS. Image from verified customer review.](http://images.novusbio.com/fullsize/HLA-DR-Antibody-L243-Flow-Cytometry-NB100-77855-img0012.jpg)
![Flow Cytometry: HLA-DR Antibody (L243) [NB100-77855] - Analysis using the Biotin conjugate of NB100-77855. Staining of Human peripheral blood lymphocytes with biotinylated L243, followed by Sav-PE.](http://images.novusbio.com/fullsize/HLA-DR-Antibody-L243-Flow-Cytometry-NB100-77855-img0013.jpg)
![Flow Cytometry: HLA-DR Antibody (L243) [NB100-77855] - Analysis using the DyLight 405 conjugate of NB100-77855. Staining of HLA-DR in human PBMCs using anti-HLA-DR antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/HLA-DR-Antibody-L243-Flow-Cytometry-NB100-77855-img0014.jpg)
![Western Blot: HLA-DR Antibody (LN-3) [NBP2-45310] - Western Blot Analysis of Ramos cell lysate using HLA-DR antibody (LN-3).](http://images.novusbio.com/fullsize/HLA-DR-Antibody-LN-3-Western-Blot-NBP2-45310-img0014.jpg "Western Blot: HLA-DR Antibody (LN-3) [NBP2-45310] - Western Blot Analysis of Ramos cell lysate using HLA-DR antibody (LN-3).")
![Immunocytochemistry/Immunofluorescence: HLA-DR Antibody (LN-3) [NBP2-45310] - Immunofluorescence staining of Ramos cells using HLA-DRMouse Monoclonal Antibody (LN-3) followed by goat anti-Mouse IgG-CF488 (green). Nuclei are stained with Reddot.](http://images.novusbio.com/fullsize/HLA-DR-Antibody-LN-3-Immunocytochemistry-Immunofluorescence-NBP2-45310-img0011.jpg)
![Immunohistochemistry-Paraffin: HLA-DR Antibody (LN-3) [NBP2-45310] - Human Histiocytoma stained with HLA-DRB Monoclonal Antibody (LN-3).](http://images.novusbio.com/fullsize/HLA-DR-Antibody-LN-3-Immunohistochemistry-Paraffin-NBP2-45310-img0004.jpg)
![Flow Cytometry: HLA-DR Antibody (LN-3) [NBP2-45310] - Flow Cytometric Analysis of Raji cells. HLA-DR Mouse Monoclonal Antibody (LN-3) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/HLA-DR-Antibody-LN-3-Flow-Cytometry-NBP2-45310-img0012.jpg)
![Western Blot: HLA-DR Antibody (LN-3) [NBP2-45310] - Flow Cytometry: HLA-DR Antibody (LN-3) - Azide and BSA Free [NBP2-34676] - Analysis using the PE conjugate of NBP2-34676. Staining of normal human peripheral blood cells with Mouse IgG2b K Isotype Control PE (blue histogram) or Anti-Human HLA-DR PE (purple histogram). Cells in the lymphocyte (left) or monocyte (right) gate were used for analysis.](http://images.novusbio.com/fullsize/HLA-DR-Antibody-LN-3-Western-Blot-NBP2-45310-img0007.jpg)
![Western Blot: HLA-DR Antibody (LN-3) [NBP2-45310] - Western Blot Analysis of Ramos cell and human spleen lysate using HLA-DR Mouse Monoclonal Antibody (LN-3).](http://images.novusbio.com/fullsize/HLA-DR-Antibody-LN-3-Western-Blot-NBP2-45310-img0013.jpg)
![Immunohistochemistry-Paraffin: HLA-DR Antibody (LN-3) [NBP2-45310] - Human Tonsil stained with HLA-DRB Monoclonal Antibody (LN-3).](http://images.novusbio.com/fullsize/HLA-DR-Antibody-LN-3-Immunohistochemistry-Paraffin-NBP2-45310-img0003.jpg)
![Flow Cytometry: HLA-DR Antibody (LN-3) [NBP2-45310] - Flow Cytometry: HLA-DR Antibody (LN-3) - Azide and BSA Free [NBP2-34676] - Analysis using the PE/Cy7 conjugate of NBP2-34676. Staining of HLA-DR in human buffy coat using anti-HLA-DR antibody. The primary antibody was used at a dilution of 1:100 and incubated for 25 min at 4C in 2% human serum, 0.5mM EDTA in DPBS. Image from verified customer review.](http://images.novusbio.com/fullsize/HLA-DR-Antibody-LN-3-Flow-Cytometry-NBP2-45310-img0010.jpg)
DR555MS) and counterstained with DAPI (blue; Lunaphore Catalog # 
DR555MS) and counterstained with DAPI (blue; Lunaphore Catalog # 
HAF018). A specific band was detected for HLA‑DR at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using 
VC001) or the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # 
![Western Blot: HLA-DR Antibody (4Y9Y8) [NBP3-15337] - Analysis of extracts of Raji cells, using HLA-DR Rabbit mAb (NBP3-15337) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/HLA-DR-Antibody-4Y9Y8-Western-Blot-NBP3-15337-img0002.jpg "Western Blot: HLA-DR Antibody (4Y9Y8) [NBP3-15337] - Analysis of extracts of Raji cells, using HLA-DR Rabbit mAb (NBP3-15337) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunohistochemistry: HLA-DR Antibody (4Y9Y8) [HLA-DR] - Immunohistochemistry analysis of HLA-DR in paraffin-embedded human tonsil tissue using HLA-DR Rabbit mAb at a dilution of 1:1600 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15337_rabbit-hla-dr-mab-4y9y8-5220251718983.jpg)
![Immunohistochemistry: HLA-DR Antibody (4Y9Y8) [HLA-DR] - Immunohistochemistry analysis of HLA-DR in paraffin-embedded human liver tissue using HLA-DR Rabbit mAb at a dilution of 1:1600 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15337_rabbit-hla-dr-mab-4y9y8-52202517251958.jpg)
![Immunohistochemistry: HLA-DR Antibody (10-D8-R) [NBP3-32435] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Mouse anti-HLA-DR antibody (NBP3-32435) at 1/1,000 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32435) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32435_mouse-hla-dr-mab-10-d8-r-298202414262655.jpg "Immunohistochemistry: HLA-DR Antibody (10-D8-R) [NBP3-32435] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Mouse anti-HLA-DR antibody (NBP3-32435) at 1/1,000 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32435) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunohistochemistry: HLA-DR Antibody (10-D8-R) [NBP3-32435] - Immunofluorescence analysis of paraffin-embedded human stomach cancer tissue labeling HLA-DR with Mouse anti-HLA-DR antibody (NBP3-32435) at 1/100 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32435, green) at 1/100 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32435_mouse-hla-dr-mab-10-d8-r-298202414161847.jpg)
![Immunocytochemistry/ Immunofluorescence: HLA-DR Antibody (10-D8-R) [NBP3-32435] - Immunocytochemistry analysis of Daudi cells labeling HLA-DR with Mouse anti-HLA-DR antibody (NBP3-32435) at 1/100 dilution.<br/><br/> Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-HLA-DR antibody (NBP3-32435) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br/><br/> beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32435_mouse-hla-dr-mab-10-d8-r-2920241192357.jpg)
