HLA DQ Antibody (K274.3G8) - BSA Free

Flow Cytometry: HLA DQ Antibody (K274.3G8) [NBP3-11305] - Staining of porcine peripheral blood lymphocytes with Mouse anti Pig SLA Class II D Q followed by Goat anti Mouse IgG (H/L):FITC.

• Reactivity: Po
• Applications: Flow, IHC
• Clone: K274.3G8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

HLA DQ Antibody (K274.3G8) - BSA Free Summary

• Description: Novus Biologicals Mouse HLA DQ Antibody (K274.3G8) - BSA Free (NBP3-11305) is a monoclonal antibody validated for use in IHC and Flow. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Porcine peripheral blood lymphocytes
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: HLA-DQA1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10 - 1:100
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
• Application Notes: Flow Cytometry: Use 10 uL of the suggested working dilution to label 1x10^6 cells in 100 uL

Reactivity Notes

Predicted cross-reactivities: Bovine

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.09% Sodium Azide
• Purity: Protein A purified

Alternate Names for HLA DQ Antibody (K274.3G8) - BSA Free

• CD
• CELIAC1DQ alpha 1 chain
• DC-1 alpha chain
• DQ-A1
• FLJ27088
• FLJ27328
• GSE
• HLA class II histocompatibility antigen, DQ(W3) alpha chain
• HLA-DCA
• HLA-DQA
• leucocyte antigen DQA1
• leukocyte antigen alpha chain
• major histocompatibility complex, class II, DQ alpha 1
• MGC149527
• MHC class II antigen
• MHC class II DQA1
• MHC class II HLA-D alpha glycoprotein
• MHC class II HLA-DQ-alpha-1
• MHC class II surface glycoprotein
• MHC HLA-DQ alpha

Background

The HLADQ1 monoclonal antibody recognizes human HLA-DQ also known as MHC class II DQ monomorphic antigen. The major histocompatability complex is composed of two heterodimeric glycoproteins ( alpha and beta chains) with apparent molecular weights of 27 and 32 kD. In contrast to other MHC class II molecules, both polypeptide chains of HLA-DQ are polymorphic, with the alpha chain showing an extremely high degree of polymorphism. HLA-DQ is expressed on B cells in the peripheral blood, weakly on activated T cells and some monocytes. HLA-DQ is absent on hematopoietic progenitors, resting T cells, erythrocytes and platelets. HLA-DQ is expressed after HLA-DR and HLA-DP in hematopoietic development. HLA-DQ presents peptide fragments mainly from degraded intravesicular and extracellular proteins to CD4+ T lymphocytes. Specific alleles of HLA-DQ have been linked to the pathogenesis of several autoimmune diseases (including diabetes), both as a susceptibility and resistance factor depending on the particular polymorphism. The HLADQ1 antibody has been reported to be useful for flow cytometric detection of human HLA-DQ and immunohistochemistry (acetone-fixed frozen tissues and formalin-fixed paraffin-embedded tissues).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HLA-DQA1