HLA DP Antibody (HI43)

Product Discontinued

HLA DP Antibody (HI43) Summary

• Immunogen: The immunogen was the full length version of the protein: HLA-DP (HI43)
• Specificity: HLA-DP (HI43)
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: HLA-DPA1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunohistochemistry
• Application Notes: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is: 1.0 microgram per one million cells in 100 microliter volume or 100 microliter of whole blood. Immunohistochemistry-frozen/paraffin. It is recommended that the reagent be titrated for optimal performance for each application.

Reactivity Notes

Human.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.2)
• Preservative: 0.09% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for HLA DP Antibody (HI43)

• DP alpha 1 chain
• HLA class II histocompatibility antigen, DP alpha chain
• HLADP
• HLA-SB alpha chain
• major histocompatibility complex, class II, DP alpha 1
• MHC class II antigen
• MHC class II DP3-alpha
• MHC class II DPA1
• MHC class II HLA-DPA1 antigen
• Primed lymphocyte test-1

Background

The HI43 monoclonal antibody recognizes human HLA-DP also known as MHC class II DP monomorphic antigen. The major histocompatability complex is composed of two heterodimeric glycoproteins ( alpha and beta chains) with apparent molecular weights of 28 and 34 kD. HLA-DP is expressed on hematopoietic progenitors, B cells, monocytes and macrophages, dendritic cells, Langerhans cells, activated T cells, and some endothelial and epithelial cells. HLA-DP is not expressed on resting T cells, erythrocytes and platelets. HLA-DP is highly expressed on some B cell leukemias and some chronic myelogenous leukemias, may be useful in the diagnosis and classification of leukemias. HLA-DP presents peptide fragments mainly from degraded intravesicular and extracellular proteins to CD4+ lymphocytes. Specific HLA-DP alleles have implicated in the pathogenesis of several autoimmune diseases including juvenile rheumatoid arthritis and chronic immune disorders such as berylliosis. The HI43 antibody has been reported to be useful for flow cytometric detection of human HLA-DP and immunohistochemistry (acetone-fixed frozen tissues and formalin-fixed paraffin-embedded tissues).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HLA-DPA1
• Entrez:
  • 3113(Human)