HLA Class I Antibody (MEM-123) - BSA Free

Flow Cytometry: HLA Class I Antibody (MEM-123) [NB500-505] - Surface staining of human peripheral blood with anti-HLA-class I (MEM-123) purified, GAM-APC.

Flow Cytometry: HLA Class I Antibody (MEM-123) [NB500-505] - A surface stain was performed on human peripheral blood lymphocytes with HLA Class I (MEM-123) antibody NB500-505 (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0101B, R&D Systems).

• Reactivity: Hu, Bv, Pm
• Applications: ELISA, Flow, IP, CyTOF-ready
• Clone: MEM-123
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

HLA Class I Antibody (MEM-123) - BSA Free Summary

• Immunogen: COS-7 African green monkey kidney cells
• Specificity: This antibody (clone MEM-123) reacts with all human classical MHC Class I molecules (major histocompatibility complex) in native cell-surface forms as well as with human HLA-G cDNA transfected cells. MHC Class I molecules (MHC Class Ia) are expressed on the surface of all human cell types. This antibody completely blocks binding of classical W6/32 to surface-expressed HLA-G, but does not cross-blocks This antibody MEM-G/9.
• Isotype: IgG3
• Clonality: Monoclonal
• Host: Mouse
• Gene: HLA-A
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • ELISA 1:2000
  • Flow Cytometry 4 ug/ml
  • Immunoprecipitation 1:50
• Application Notes: This antibody is Cytof ready.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris buffered saline (TBS), pH 8.0
• Preservative: 15mM Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A purified

Alternate Names for HLA Class I Antibody (MEM-123) - BSA Free

• A-10 alpha chain
• FLJ26655
• HLA class I histocompatibility antigen, A-1 alpha chain
• HLA class I histocompatibility antigen, A-28 alpha chain
• HLA class I histocompatibility antigen, A-9 alpha chain
• HLA Class I
• HLAA
• HLA-A
• HLA-ABC
• major histocompatibility complex, class I, A
• MHC class I antigen A*1
• MHC class I antigen A*11
• MHC class I antigen A*80

Background

HLA-class I major histocompatibility (MHC) antigens are intrinsic membrane glycoproteins expressed on nucleated cells and noncovalently associated with an invariant beta2 microglobulin. They carry foreign determinants important for immune recognition by cytotoxic T cells, thus important for anti-viral and anti-tumour defence. Human HLA-class I antigens are represented by HLA-A, HLA-B and HLA-C molecules.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HLA-A
• Entrez:
  • 3106(Human)
  • 3107(Human)