HLA Class I Antibody (mAb90) - Azide and BSA Free

Functional: HLA Class I Antibody (mAb90) [DDX0250P-100] - mAb90 inhibits CD40-dependent B-cell proliferation. Tonsil B cells were cultured for 5 days on CD32-L cellsi n the presence of 1ug/ml anti-CD40 mAb with 1ug/ml of W6/32 or mAb90.

Functional: HLA Class I Antibody (mAb90) [DDX0250P-100] - mAb90 induces apoptosis of CD40-activated B cells. Cells were collected after 12 hours of treatment with the different mAbs and DNA from 2X104 cells was runon 2% agarose gel and stainedwith ethidiumbromide.

• Reactivity: Hu
• Applications: ELISA, Flow, Func
• Clone: mAb90
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 0.5 mg/ml

HLA Class I Antibody (mAb90) - Azide and BSA Free Summary

• Immunogen: HLA-A1 was used as immunogen.
• Specificity: human alpha1domain of HLA class I molecule
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: HLA-A
• Purity: Ion exchange chromatography

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Flow Cytometry 1:10-1:1000
  • Functional

Reactivity Notes

Human

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris-NaCl (pH 8.0)
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: Ion exchange chromatography

Notes

This product is manufactured by Eurobio Scientific (formerly Dendritics) and distributed by Novus Biologicals.

Alternate Names for HLA Class I Antibody (mAb90) - Azide and BSA Free

• A-10 alpha chain
• FLJ26655
• HLA class I histocompatibility antigen, A-1 alpha chain
• HLA class I histocompatibility antigen, A-28 alpha chain
• HLA class I histocompatibility antigen, A-9 alpha chain
• HLA Class I
• HLAA
• HLA-A
• HLA-ABC
• major histocompatibility complex, class I, A
• MHC class I antigen A*1
• MHC class I antigen A*11
• MHC class I antigen A*80

Background

HLA CLass I is involved in the presentation of foreign antigens to the immune system. This antibody is a valuable reagent for analysing variations in HLA class I expression in different disease states e.g. liver disease, muscular dystrophy, inflammatory myopathy and other neuromuscular disorders. This antibody is also suitable as a positive control for HLA tissue typing and crossmatching.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HLA Class I Antibody (DDX0250P-100)(2)

Publications using DDX0250P-100

• Genestier L et al. Caspase-dependent ceramide production in Fas- and HLA class I-mediated peripheral T cell apoptosis. J Biol Chem 1998-02-01 [PMID: 9478956]
• Genestier L et al. Antibodies to HLA class I alpha1 domain trigger apoptosis of CD40-activated human B lymphocytes. Blood 1997-07-01 [PMID: 9226173]

Bioinformatics

• Gene Symbol: HLA-A