HLA ABC Antibody (YTH862.2) - BSA Free

Flow Cytometry: HLA ABC Antibody (YTH862.2) [NB100-65411] - Staining of human peripheral blood lymphocytes.

• Reactivity: Hu
• Applications: Flow, Func, IHC, IP
• Clone: YTH862.2
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

HLA ABC Antibody (YTH862.2) - BSA Free Summary

• Immunogen: PHA activated human peripheral blood lymphocytes
• Specificity: NB100-65411 recognizes a monomorphic determinant on the human HLA Class I heavy chain. Epitope analysis has shown that this antibody binds in the alpha1 domain. MHC Class I is expressed by all nucleated cells. Recent studies have shown that this antibody may be able to induce apoptosis in activated but not resting T cells (1).
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Gene: HLA-A
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Functional
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunoprecipitation 1:10-1:500
• Application Notes: Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul. This product contains sodium azide, removal by dialysis is recommended prior to use in functional assays.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for HLA ABC Antibody (YTH862.2) - BSA Free

• A-10 alpha chain
• FLJ26655
• HLA class I histocompatibility antigen, A-1 alpha chain
• HLA class I histocompatibility antigen, A-28 alpha chain
• HLA class I histocompatibility antigen, A-9 alpha chain
• HLAA
• major histocompatibility complex, class I, A
• MHC class I antigen A*1
• MHC class I antigen A*11
• MHC class I antigen A*80

Background

Involved in the presentation of foreign antigens to the immune system.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HLA ABC Antibody (NB100-65411)(1)

Publication using NB100-65411

• Hasselmann J, Coburn M, England W et al. Development of a Chimeric Model to Study and Manipulate Human Microglia In Vivo Neuron 2019-07-01 [PMID: 31375314]

Bioinformatics

• Gene Symbol: HLA-A