Histone H4 [Methyl Lys20] Antibody

Images

 
Western Blot: Histone H4 [Methyl Lys20] Antibody [NBP2-59258] - Western blot was performed on whole cell extracts (25 ug, lane 1) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 2, 3, 4 and ...read more
Immunocytochemistry/ Immunofluorescence: Histone H4 [Methyl Lys20] Antibody [NBP2-59258] - HeLa cells were stained with the antibody against H4K20me1 and with DAPI. Cells were fixed with 4% formaldehyde for 10' and ...read more
Chromatin Immunoprecipitation: Histone H4 [Methyl Lys20] Antibody [NBP2-59258] - ChIP assays were performed using human HeLa cells, the antibody against H4K20me1 and optimized PCR primer sets for qPCR. ChIP was ...read more
ELISA: Histone H4 [Methyl Lys20] Antibody [NBP2-59258] - To determine the titer, an ELISA was performed using a serial dilution of the antibody directed against H4K20me1 in antigen coated wells. The antigen used was a ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ChIP, DB, ELISA, ICC/IF, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Histone H4 [Methyl Lys20] Antibody Summary

Immunogen
The exact immunogen is proprietary information.
Modification
Methyl Lys20
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HIST4H4
Purity
Peptide affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Chromatin Immunoprecipitation 1 - 2 ug
  • Dot Blot 1:20000
  • ELISA 1:1000
  • Immunocytochemistry/Immunofluorescence 1:500
  • Immunofluorescence 1:500

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide and 0.05% ProClin 300
Purity
Peptide affinity purified

Alternate Names for Histone H4 [Methyl Lys20] Antibody

  • H4
  • H4/A
  • H4/B
  • H4/C
  • H4/D
  • H4/E
  • H4/G
  • H4/H
  • H4/I
  • H4/J
  • H4/K
  • H4/M
  • H4/N
  • H4/O
  • H4/p
  • H4F2
  • H4FA
  • H4FB
  • H4FC
  • H4FD
  • H4FE
  • H4FG
  • H4FH
  • H4FI
  • H4FJ
  • H4FK
  • H4FM
  • H4FN
  • H4FO
  • H4K20Me
  • H4M
  • HIST1H4A
  • HIST1H4B
  • HIST1H4C
  • HIST1H4D
  • HIST1H4E
  • HIST1H4F
  • HIST1H4H
  • HIST1H4I
  • HIST1H4J
  • HIST1H4K
  • HIST1H4L
  • HIST2H4
  • HIST2H4A
  • HIST2H4B
  • HIST4H4
  • histone 4, H4
  • Histone Cluster 1 H4
  • Histone Cluster 1 H4i
  • Histone Cluster 4 H4
  • histone cluster 4, H4
  • Histone H4
  • MGC24116

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ChIP Webinar
ChIP Video Protocol
ICC/IF Video Protocol

FAQs for Histone H4 Antibody (NBP2-59258) (0)

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Secondary Antibodies

 

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H4 - Monitoring global chromatin structure through histone modifications
Histones make up the main protein component of chromatin and are responsible for storing and organizing the genome in a compact yet accessible manner. In addition to storage, histones play an important role in the regulation of various cellular pro...  Read full blog post.

Histone H4 Phosphorylation: Affecting Liver Regeneration and Cancer
Histones are highly conserved proteins that function in the organization of nuclear DNA to create chromatin in eukaryotic cells. Post-translational alterations of histones are critical to monitoring and regulating DNA structure, expression, and gene t...  Read full blog post.

Histone H4: Implications in Liver Cancer
Histones are highly conserved proteins that function in the organization of nuclear DNA to create chromatin in eukaryotic cells. Post-translational alterations of histones are critical to monitoring and regulating DNA structure, expression, and gene t...  Read full blog post.

"Come Fly with Me" - New Drosophila Model Developed for Direct in Vivo Study of Histones
Forming the major protein component of chromatin, histones are essential to the structure and organization of chromosomes, forming the nucleosome around which DNA is packaged and wrapped.Antibody studies have revealed histones undergo various posttr...  Read full blog post.

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Bioinformatics

Gene Symbol HIST4H4