HIST3H3 Antibody (4E9B11) - BSA Free

Western Blot: HIST3H3 Antibody (4E9B11) [NBP2-52482] - Analysis using AHH3 mouse mAb against NIH3T3 (1), Hela (2), K562 (3) cell lysate.

Flow Cytometry: HIST3H3 Antibody (4E9B11) [NBP2-52482] - Analysis of NIH/3T3 cells using HH3 mouse mAb (green) and negative control (red).

ELISA: HIST3H3 Antibody (4E9B11) [NBP2-52482] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);

• Reactivity: Hu, Mu
• Applications: WB, ELISA, Flow, CyTOF-ready
• Clone: 4E9B11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

HIST3H3 Antibody (4E9B11) - BSA Free Summary

• Immunogen: Synthesized peptide of human HIST3H3 (AA: ARTKQTAR(AcK)STG-C).
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: H3-4
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Western Blot 1:500-1:2000
• Application Notes: This antibody is Cytof ready.
• Theoretical MW: 15.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for HIST3H3 Antibody (4E9B11) - BSA Free

• H3 histone family, member T
• H3.4
• H3/G
• H3/gH3.4
• H3/T
• H3FT
• H3FTMGC126888
• H3t
• H3tMGC126886
• HIST3H3
• Histone 3, H3
• histone cluster 3, H3
• histone H3.1t

Background

Histones are nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber as they play a critical role in transcription regulation, DNA repair, DNA replication, and chromosomal stability. The HIST3H3 gene is intronless and encodes a 136 amino acid long, 15 kDA histone H3.1t protein that lacks polyA tails and obtain a palindromic termination element. The HIST3H3 gene is located separately from the other H3 genes on the cluster on chromosome 6p22-p21.3. HIST3H3 participates in PKA signaling, development notch signaling pathway, histone modification, and interferon pathway through interactions with genes HIST1H4B, HIST4H4, HIST1H4A, HIST1H4C, and HIST1H4D. HIST3H3 has been researched regarding its role in immunodeficiency, leukemia, prostatitis, huntington's disease, lupus, multiple sclerosis, laryngeal cancer, and lymphomas.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: H3-4