PSMC3 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: PSMC3 Antibody [NBP1-86962] - Staining of human cell line U-251 MG shows localization to nuclear bodies & cytosol. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: PSMC3 Antibody [NBP1-86962] - Staining of human cerebral cortex shows strong nuclear and cytoplasmic positivity in neuronal cells.

Simple Western: PSMC3 Antibody [NBP1-86962] - Mouse whole-spleen lysates at the indicated concentrations were probed with a 1:25 dilution of this PSMC3 antibody. Simple Western image submitted by a verified customer review.

Western Blot: PSMC3 Antibody [NBP1-86962] - Analysis of human fibroblasts. WB image submitted by a verified customer review.

Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

Analysis in human cell line MCF-7.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

PSMC3 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit PSMC3 Antibody - BSA Free (NBP1-86962) is a polyclonal antibody validated for use in IHC, WB, ICC/IF and Simple Western. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: EFPMPNEEARARIMQIHSRKMNVSPDVNYEELARCTDDFNGAQCKAVCVEAGMIALRRGATELTHEDYMEGILEVQAKKKANLQYY
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PSMC3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
  See Simple Western Antibody Database for Simple Western validation: Tested in Mouse whole-spleen lysates, separated by Size, antibody dilution of 1:25
• Control Peptide:

  PSMC3 Protein (NBP1-86962PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for PSMC3 Antibody - BSA Free

• human immunodeficiency virus tat transactivator binding protein-1
• MGC8487
• proteasome (prosome, macropain) 26S subunit, ATPase, 3,26S proteasome AAA-ATPase subunit RPT5
• Proteasome 26S subunit ATPase 3
• Proteasome subunit P50
• TBP-126S protease regulatory subunit 6A
• TBP1Tat-binding protein 1

Background

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the ATPase subunits, a member of the triple-A family of ATPases that have chaperone-like activity. This subunit may compete with PSMC2 for binding to the HIV tat protein to regulate the interaction between the viral protein and the transcription complex. A pseudogene has been identified on chromosome 9. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Reviews for PSMC3 Antibody (NBP1-86962) (2)

Average Rating: 4

(Based on 2 reviews)

We have 2 reviews tested in 2 species: Human, Mouse.

reviewed by: Verified Customer Simple Western Mouse 08/23/2019

Summary

• Application: Simple Western
• Sample Tested: Mouse Spleen Lysate
• Species: Mouse
• Lot: A90016

reviewed by: Verified Customer WB Human 04/19/2018

Summary

• Application: Western Blot
• Sample Tested: fibroblasts
• Species: Human
• Lot: A90016

Bioinformatics

• Gene Symbol: PSMC3