HIST1H2AC Antibody

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Western Blot: HIST1H2AC Antibody [NBP1-45619] - Analysis of Histone H2A expression with HeLa nuclear lysate.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

HIST1H2AC Antibody Summary

Immunogen
Synthetic peptide corresponding to the N-terminus of human histone H2A.
Specificity
It does not cross-react with other histones.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HIST1H2AC
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot
  • Immunohistochemistry
  • Immunoprecipitation
Application Notes
Western blotting (0.5-4 ug/ml), immunoprecipitation (10-20 ug/ml) and Immunohistochemistry (10-30 ug/ml). However, the optimal conditions should be determined individually. The antibody detects ~14 kDa histone H2A protein. It does not cross-react with other histones. HeLa cell nuclear extract can be used as a positive control.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2), 0.5% BSA and 30% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for HIST1H2AC Antibody

  • dJ221C16.4
  • H2A/l
  • H2AFLmember L
  • histone 1, H2ac
  • histone cluster 1, H2ac
  • histone H2A type 1-C
  • Histone H2A/l
  • histone H2AC
  • MGC99519

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H2A family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol HIST1H2AC
Entrez
Uniprot