HIP1 Antibody (4B10)

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Western Blot: HIP1 Antibody (4B10) [NB300-203] - Western Blot on HeLa whole cell extracts.

Product Details

Summary
Reactivity Hu, Mu, Rt(-)Species Glossary
Applications WB, ChIP, ICC/IF, IHC-P, IP
Clone
4B10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

HIP1 Antibody (4B10) Summary

Immunogen
Human 3'-HIP1 (~65 kDa)
Localization
Cytoplasm
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
HIP1
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:10000
  • Chromatin Immunoprecipitation
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
Application Notes
This HIP1 antibody is useful for Western blot, where a band at approximately 115 kDa is seen. Immunohistochemistry-Paraffin, Chromatin Immunoprecipitation and Immunoprecipitation were reported in scientific literature.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
115 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
HeLa Lysate (NB800-PC1)
Publications
Read Publications using
NB300-203 in the following applications:

  • 1 publication
  • 3 publications
  • 1 publication
  • IP
    1 publication
  • WB
    4 publications

Reactivity Notes

Human and Mouse. Does not react with rat.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.1% Sodium Azide
Purity
Unpurified

Alternate Names for HIP1 Antibody (4B10)

  • HIP-1
  • HIP-I
  • huntingtin interacting protein 1
  • huntingtin-interacting protein 1
  • Huntingtin-interacting protein I
  • ILWEQ
  • MGC126506

Background

One of the four subunits of the histone regulatory complex A, HIP1 is an anti-apoptosis protein in the brain. HIP1 (Huntingtin-interacting protein 1) protects normal neurological tissue from erroneous inflammatory and apoptotic events by linking the actin cytoskeleton and clathrin-mediated endocytosis machinery. It is important in the generation of clathrin coated vesicles in the neuron. However, if deletion of HIP1 occurs, neurological disease is initiated and is correlated to epilepsy, learning disabilities, spinal and testicular defects, hematopoietic issues, and other neurobehavioral abnormalities such as Huntington's disease. HIP1 also interacts with the Hedgehog signaling pathway, and is important in regulating the abundance and scope of Hedgehog signaling.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for HIP1 Antibody (NB300-203)(9)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 5 applications: ChIP, ICC/IF, IHC-P, IP, WB.


Filter By Application
ChIP
(1)
ICC/IF
(3)
IHC-P
(1)
IP
(1)
WB
(4)
All Applications
Filter By Species
Human
(7)
Mouse
(2)
All Species
Showing Publications 1 - 9 of 9.
Publications using NB300-203 Applications Species
Fontaine SN, Bauer SP, Lin X et al. Replacement of charged and polar residues in the coiled-coiled interface of huntingtin-interacting protein 1 (HIP1) causes aggregation and cell death FEBS Lett 2012 Jul 23 [PMID: 22835334] (ICC/IF, WB, Human) ICC/IF, WB Human
Barker BL, Benovic J. G protein-coupled receptor kinase 5 phosphorylation of hip regulates internalization of the chemokine receptor CXCR4 Biochemistry 2011 Aug 16 [PMID: 21728385] (WB, Human) WB Human
Bradley SV, Smith MR, Hyun TS, Lucas PC, Li L, Antonuk D, Joshi I, Jin F, Ross TS. Aberrant Huntingtin interacting protein 1 in lymphoid malignancies. Cancer Res;67(18):8923-31. 2007 Sep 15. [PMID: 17875735]
Bradley SV, HollEC, Liu GY, Thomas D, Hyun TS, Ross TS. Huntingtin interacting protein 1 is a novel brain tumor marker that associates with epidermal growth factor receptor. Cancer Res;67(8):3609-15. 2007 Apr 15. [PMID: 17440072] (IHC-P, Human) IHC-P Human
Mills, IG et al. Huntingtin interacting protein 1 modulates the transcriptional activity of nuclear hormone receptors. J Cell Biol;170(2):191-200. 2005 Jul 18. [PMID: 16027218] (ChIP, Human) ChIP Human
Bradley SV, Oravecz-Wilson KI, Bougeard G, Mizukami I, Li L, Munaco AJ, Sreekumar A, Corradetti MN, Chinnaiyan AM, Sanda MG, Ross TS. Serum antibodies to huntingtin interacting protein-1: a new blood test for prostate cancer. Cancer Res;65(10):4126-33. 2005 May 15. [PMID: 15899803] (WB, Human, Mouse) WB Human, Mouse
Hyun TS, Rao DS, Saint-Dic D, Michael LE, Kumar PD, Bradley SV, Mizukami IF, Oravecz-Wilson KI, Ross TS. HIP1 and HIP1r stabilize receptor tyrosine kinases and bind 3-phosphoinositides via epsin N-terminal homology domains. J Biol Chem;279(14):14294-306. 2004 Apr 2. [PMID: 14732715] (ICC/IF, Human) ICC/IF Human
Rao, DS et al. Huntingtin-interacting protein 1 is overexpressed in prostate and colon cancer and is critical for cellular survival. J Clin Invest;110(3):351-60. 2002 Aug. [PMID: 12163454]
Rao, DS et al. Huntingtin interacting protein 1 Is a clathrin coat binding protein required for differentiation of late spermatogenic progenitors. Mol Cell Biol;21(22):7796-806. 2001 Nov. [PMID: 11604514] (WB, ICC/IF, IP, Human, Mouse) WB, ICC/IF, IP Human, Mouse

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Product General Protocols

View specific protocols for HIP1 Antibody (NB300-203): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ChIP Webinar
ChIP Video Protocol
ICC/IF Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol HIP1
Entrez
Uniprot