HIP1 Antibody (1B11)

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Western Blot: HIP1 Antibody (1B11) [NB300-204] - HeLa whole cell extracts.

Product Details

Summary
Reactivity Hu, Mu, Ba, Rt(-)Species Glossary
Applications WB, ICC/IF, IP
Clone
1B11
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

HIP1 Antibody (1B11) Summary

Immunogen
Human 3'-HIP1 (~65 kDa)
Localization
Localization: Cytoplasmic
Specificity
NB300-204 is specific for human HIP1. Does not cross-react with HIP1r.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
HIP1
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:10000
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunoprecipitation
Application Notes
This HIP1 antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot, where a band is observed at ~115 kDa. Use in Immunoprecipitation reported in scientific literature (PMID 23272104)

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
115 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
HeLa Lysate (NB800-PC1)
Publications
Read Publications using
NB300-204 in the following applications:

  • 2 publications
  • IP
    1 publication
  • WB
    4 publications

Reactivity Notes

Human, mouse and bacteria. Does not appear to work in rat.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.1% Sodium Azide
Purity
Unpurified

Alternate Names for HIP1 Antibody (1B11)

  • HIP-1
  • HIP-I
  • huntingtin interacting protein 1
  • huntingtin-interacting protein 1
  • Huntingtin-interacting protein I
  • ILWEQ
  • MGC126506

Background

One of the four subunits of the histone regulatory complex A, HIP1 is an anti-apoptosis protein in the brain. HIP1 (Huntingtin-interacting protein 1) protects normal neurological tissue from erroneous inflammatory and apoptotic events by linking the actin cytoskeleton and clathrin-mediated endocytosis machinery. It is important in the generation of clathrin coated vesicles in the neuron. However, if deletion of HIP1 occurs, neurological disease is initiated and is correlated to epilepsy, learning disabilities, spinal and testicular defects, hematopoietic issues, and other neurobehavioral abnormalities such as Huntington's disease. HIP1 also interacts with the Hedgehog signaling pathway, and is important in regulating the abundance and scope of Hedgehog signaling.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for HIP1 Antibody (NB300-204)(7)

We have publications tested in 3 confirmed species: Human, Mouse, Bacteria.

We have publications tested in 3 applications: ICC/IF, IP, WB.


Filter By Application
ICC/IF
(2)
IP
(1)
WB
(4)
All Applications
Filter By Species
Human
(5)
Mouse
(1)
Bacteria
(1)
All Species
Showing Publications 1 - 7 of 7.
Publications using NB300-204 Applications Species
Choudhury KR, Raychaudhuri S, Bhattacharyya NP. Identification of HYPK-interacting proteins reveals involvement of HYPK in regulating cell growth, cell cycle, unfolded protein response and cell death. PLoS One. 2012 Dec 10 [PMID: 23272104] (IP, Human) IP Human
Datta M, Choudhury A, Lahiri A, Bhattacharyya NP. Genome wide gene expression regulation by HIP1 Protein Interactor, HIPPI: Prediction and validation. BMC Genomics;12(1):463. 2011 Sep 26. [PMID: 21943362]
Datta M, Bhattacharyya NP. Regulation of RE1 Protein Silencing Transcription Factor (REST) Expression by HIP1 Protein Interactor (HIPPI). J Biol Chem;286(39):33759-33769. 2011 Sep 30. [PMID: 21832040] (WB, Human) WB Human
Banerjee M, Datta M, Majumder P et al. Transcription regulation of caspase-1 by R393 of HIPPI and its molecular partner HIP-1. Nucleic Acids Res. 2009 Nov 24. [PMID: 19934260] (WB, ICC/IF, Human) WB, ICC/IF Human
Mills, IG et al. Huntingtin interacting protein 1 modulates the transcriptional activity of nuclear hormone receptors. J Cell Biol;170(2):191-200. 2005 Jul 18. [PMID: 16027218] (WB, Bacteria) WB Bacteria
Rao, DS et al. Huntingtin-interacting protein 1 is overexpressed in prostate and colon cancer and is critical for cellular survival. J Clin Invest;110(3):351-60. 2002 Aug. [PMID: 12163454] (WB, Mouse, Human) WB Mouse, Human
Rao DS, Chang JC, Kumar PD, Mizukami I, Smithson GM, Bradley SV, Parlow AF, Ross TS. Huntingtin interacting protein 1 Is a clathrin coat binding protein required for differentiation of late spermatogenic progenitors. Mol Cell Biol;21(22):7796-806. 2001 Nov. [PMID: 11604514] (ICC/IF, Human) ICC/IF Human

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Product General Protocols

View specific protocols for HIP1 Antibody (NB300-204): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol HIP1
Entrez
Uniprot