![Immunohistochemistry-Paraffin: Hip Antibody (HL1969) - Azide and BSA Free [NBP3-25506] - HHIP antibody [HL1969] detects HHIP protein at cell membrane and secreted by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. HHIP stained by HHIP antibody [HL1969] (NBP3-25506) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25506_rabbit-hip-mab-hl1969-azide-and-bsa-free-2120249362721.jpg "Immunohistochemistry-Paraffin: Hip Antibody (HL1969) - Azide and BSA Free [NBP3-25506] - HHIP antibody [HL1969] detects HHIP protein at cell membrane and secreted by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. HHIP stained by HHIP antibody [HL1969] (NBP3-25506) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
| Reactivity | Hu, Mu, Rt, Bv, RMSpecies Glossary |
| Applications | WB, IHC |
| Clone | HL1969 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Description | Novus Biologicals Rabbit Hip Antibody (HL1969) - Azide and BSA Free (NBP3-25506) is a recombinant monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Recombinant fragment of human Hip |
| Source | HEK293 |
| Predicted Species | Rat (98%), Rhesus Macaque (100%), Bovine (98%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | HHIP |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Theoretical MW | 79 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | HHIP |
![Western Blot: Hip Antibody (HL1969) - Azide and BSA Free [NBP3-25506] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HHIP antibody [HL1969] (NBP3-25506) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25506_rabbit-hip-mab-hl1969-azide-and-bsa-free-212024937498.jpg "Western Blot: Hip Antibody (HL1969) - Azide and BSA Free [NBP3-25506] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HHIP antibody [HL1969] (NBP3-25506) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: Hip Antibody (HL1969) - Azide and BSA Free [NBP3-25506] - HHIP antibody [HL1969] detects HHIP protein at cell membrane and secreted by immunohistochemical analysis. Sample: Paraffin-embedded mouse duodenum. HHIP stained by HHIP antibody [HL1969] (NBP3-25506) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25506_rabbit-hip-mab-hl1969-azide-and-bsa-free-212024943556.jpg "Immunohistochemistry-Paraffin: Hip Antibody (HL1969) - Azide and BSA Free [NBP3-25506] - HHIP antibody [HL1969] detects HHIP protein at cell membrane and secreted by immunohistochemical analysis. Sample: Paraffin-embedded mouse duodenum. HHIP stained by HHIP antibody [HL1969] (NBP3-25506) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Immunohistochemistry-Paraffin: Hip Antibody (HL1969) - Azide and BSA Free [NBP3-25506] - HHIP antibody [HL1969] detects HHIP protein at cell membrane and secreted by immunohistochemical analysis. Sample: Paraffin-embedded human hepatocellular carcinoma. HHIP stained by HHIP antibody [HL1969] (NBP3-25506) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25506_rabbit-hip-mab-hl1969-azide-and-bsa-free-212024941444.jpg "Immunohistochemistry-Paraffin: Hip Antibody (HL1969) - Azide and BSA Free [NBP3-25506] - HHIP antibody [HL1969] detects HHIP protein at cell membrane and secreted by immunohistochemical analysis. Sample: Paraffin-embedded human hepatocellular carcinoma. HHIP stained by HHIP antibody [HL1969] (NBP3-25506) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Western Blot: Hip Antibody (HL1969) - Azide and BSA Free [NBP3-25506] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HHIP antibody [HL1969] (NBP3-25506) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25506_rabbit-hip-mab-hl1969-azide-and-bsa-free-2120249435526.jpg "Western Blot: Hip Antibody (HL1969) - Azide and BSA Free [NBP3-25506] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HHIP antibody [HL1969] (NBP3-25506) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")