HIBADH Antibody

Western Blot: HIBADH Antibody [NBP2-16827] - Sample (30 ug of whole cell lysate) A: A549 10% SDS PAGE gel, diluted at 1:5000.

Immunohistochemistry-Paraffin: HIBADH Antibody [NBP2-16827] - Immunohistochemical analysis of paraffin-embedded Breast ca, using antibody at 1:250 dilution.

Western Blot: HIBADH Antibody [NBP2-16827] - A. 30 ug GL261 whole cell lysate/extract 7. 5 % SDS-PAGE HIBADH antibody dilution: 1:1000.

• Reactivity: Hu, Mu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

HIBADH Antibody Summary

• Description: Novus Biologicals Rabbit HIBADH Antibody (NBP2-16827) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human HIBADH. The exact sequence is proprietary.
• Localization: Mitochondrion
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HIBADH
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:1000-1:10000
• Theoretical MW: 35 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for HIBADH Antibody

• 3-hydroxyisobutyrate dehydrogenase
• 3-hydroxyisobutyrate dehydrogenase, mitochondrial
• EC 1.1.1,3'-hydroxyisobutyrate dehydrogenase
• EC 1.1.1.31
• MGC40361
• NS5ATP1

Background

3-hydroxyisobutyrate dehydrogenase (3-hydroxy-2-methylpropanoate:NAD(+) oxidoreductase, EC 1.1.1.31) is a dimeric mitochondrial enzyme that catalyzes the NAD(+)-dependent, reversible oxidation of 3-hydroxyisobutyrate, an intermediate of valine catabolism, to methylmalonate semialdehyde.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: HIBADH
• Uniprot:
  • P31937(Human)