Hepsin Antibody

Western blot shows lysates of human prostate tissue, human liver tissue, LNCaP human prostate cancer cell line, and PC-3 human prostate cancer cell line. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human Hepsin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4776) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Hepsin at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 7.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

Hepsin Antibody Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Hepsin
  Ser46-Leu417 with Asp161Glu and Arg162Lys substitutions
  Accession # P05981
• Specificity: Detects human Hepsin in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: HPN
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Hepsin Antibody

• EC 3.4.21
• EC 3.4.21.106
• Hepsin
• HPN
• serine protease hepsin catalytic chain
• serine protease hepsin
• TMPRSS1
• TMPRSS1serine protease hepsin non-catalytic chain
• Transmembrane protease serine 1
• transmembrane protease, serine 1

Background

Hepsin, also known as TMPRSS1, is a Type II membrane protein with an extracellular serine protease domain (1). It is most highly expressed in liver, but is also present in many other tissues, notably lung, kidney, and skeletal muscle (2). A soluble form of Hepsin lacking the transmembrane domain has been identified (3). Hepsin is capable of activating Factor VII, and may initiate blood coagulation at the cell surface (4). Hepsin is overexpressed in various human tumors, including prostate (5), and is considered to be a biomarker for prostate cancer (6).

1. Leytus, S.P. et al. (1988) Biochemistry 27:1067.
2. Tsuji, A. et al. (1991) J. Biol. Chem. 266:16948.
3. Li, Y. et al. (2005) Biomed. Biochim. Acta 1681:157.
4. Kazama, Y. et al. (1995) J. Biol. Chem. 270:66.
5. Dhanasekaran, S.M. et al. (2001) Nature 412:822.
6. Wu, Q. and Parry, G. (2007) Front. Biosci. 12:5052.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Hepsin Antibody (AF4776)(2)

Publications using AF4776

• Denis Belitškin, Shishir M Pant, Pauliina Munne, Ilida Suleymanova, Kati Belitškina, Hanna‐Ala Hongisto et al. Hepsin regulates TGF beta signaling via fibronectin proteolysis EMBO reports 2021-11-04 [PMID: 34515392]
• Ramona Willbold, Katharina Wirth, Thomas Martini, Holger Sültmann, Christian Bolenz, Rainer Wittig Excess hepsin proteolytic activity limits oncogenic signaling and induces ER stress and autophagy in prostate cancer cells Cell Death & Disease 2019-08-09 [PMID: 31399560]

Bioinformatics

• Gene Symbol: HPN
• Uniprot:
  • P05981()