Matriptase/ST14 Antibody


PC‑3 human prostate cancer cell line was stained with Sheep Anti-Human Matriptase/ST14 Catalytic Domain Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF3946, filled histogram) or control antibody (Catalog more

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Reactivity HuSpecies Glossary
Applications WB, CyTOF-ready, Flow

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Matriptase/ST14 Antibody Summary

E. coli-derived recombinant human Matriptase/ST14 Catalytic Domain
Accession # Q9Y5Y6
Detects human Matriptase/ST14 Catalytic Domain in direct ELISAs and Western blots. In direct ELISAs, approximately 60% cross-reactivity with recombinant mouse Matriptase/Epithin is observed.
Immunogen affinity purified
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • CyTOF-ready
  • Intracellular Staining by Flow Cytometry 2.5 ug/10^6 cells
  • Western Blot 0.1 ug/mL
Read Publications using
AF3946 in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
No Preservative
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Matriptase/ST14 Antibody

  • EC 3.4.21
  • Epithin
  • HAI
  • Matriptase
  • Membrane-type serine protease 1
  • MTSP1
  • MT-SP1EC
  • prostamin
  • PRSS14
  • Serine protease 14
  • Serine protease TADG-15
  • SNC19
  • SNC19MTSP1
  • ST14
  • suppression of tumorigenicity 14 (colon carcinoma)
  • suppression of tumorigenicity 14 (colon carcinoma, matriptase, epithin)
  • suppressor of tumorigenicity 14 protein
  • TADG15
  • TADG-15
  • TMPRSS14
  • tumor associated differentially expressed gene 15 protein
  • Tumor-associated differentially-expressed gene 15 protein


Human matriptase, encoded by the ST14 (suppression of tumorogenicity 14) gene, is also known as tumor associated differentially expressed gene 15 protein/TADG‑15), epithin, and membrane-type serine protease 1/MT-SP1 (1). Predicted to have a significant role in tumor biology, matriptase may be a novel target for anti-cancer therapy (2). However, expressed in most human epithelia, matriptase is also important in several physiological processes (1). For example, it activates prostasin to initiate a protease cascade that is essential for epidermal differentiation (3), and it converts a single-chain IGFBP-rp1 into the two-chain form (4).

Matriptase is a type II transmembrane serine protease with a complex modular structure (1). The 855 amino acid (aa) sequence of human matriptase consists of a cytoplasmic tail (aa 1-55), a transmembrane domain (aa 56-76), and an extracellular portion (aa 77-855). The latter contains the following domains: SEA (aa 86-201), two CUBs (aa 214-334 and 340-447), four LDLRAs (aa 452-486, 487-523, 524-560, and 566-603), and a serine protease (aa 615-855). The physiological activation of the single-chain zymogen requires the cleavage at the SEA domain within the ER or Golgi, association with HAI-1, which facilitates the transport of the protease to the cell surface, and auto-cleavage at QAR-V(615)VGG (1). The activated matriptase is inhibited by HAI-1, and the resulting HAI-1 complex can be shed from the cell surface (1). R&D Systems rhST14 corresponds to the catalytic domain, and is inhibited effectively by rhHAI-1 and rhHAI-2A (R&D Systems, Catalog # 1048-PI and 1106-PI).

  1. List, K. et al. (2006) Mol. Med. 12:1.
  2. Uhland, K. (2006) Cell. Mol. Life Sci. 63:2968.
  3. Netzel-Arnett, S. et al. (2006) J. Biol. Chem. 281:32941.
  4. Ahmed, S. et al. (2006) FEBS J. 273:615.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Matriptase/ST14 Antibody (AF3946)(14)

We have publications tested in 3 confirmed species: Human, Mouse, Drosophila.

We have publications tested in 4 applications: ICC, IHC, Simple Western, Western Blot.

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Showing Publications 1 - 10 of 14. Show All 14 Publications.
Publications using AF3946 Applications Species
CJ Wu, M Lu, X Feng, G Nakato, MC Udey Matriptase Cleaves EpCAM and TROP2 in Keratinocytes, Destabilizing Both Proteins and Associated Claudins Cells, 2020;9(4):. 2020 [PMID: 32326212] (Western Blot, Human) Western Blot Human
L Drees, T Königsmann, MHJ Jaspers, R Pflanz, D Riedel, R Schuh Conserved function of the matriptase-prostasin proteolytic cascade during epithelial morphogenesis PLoS Genet., 2019;15(1):e1007882. 2019 [PMID: 30601807] (IHC, Drosophila) IHC Drosophila
S Friis, D Tadeo, SM Le-Gall, HJ Jürgensen, KU Sales, E Camerer, TH Bugge Matriptase zymogen supports epithelial development, homeostasis and regeneration BMC Biol., 2017;15(1):46. 2017 [PMID: 28571576] (Western Blot, Mouse) Western Blot Mouse
MS Buzza, TA Johnson, GD Conway, EW Martin, S Mukhopadhy, T Shea-Donoh, TM Antalis Inflammatory cytokines down-regulate the barrier protective prostasin-matriptase proteolytic cascade early in experimental colitis J. Biol. Chem., 2017;0(0):. 2017 [PMID: 28490634] (IHC, Mouse) IHC Mouse
S Friis, DH Madsen, TH Bugge Distinct Developmental Functions of Prostasin (CAP1/PRSS8) Zymogen and Activated Prostasin J. Biol. Chem, 2016;291(6):2577-82. 2016 [PMID: 26719335] (Simple Western, Mouse) Simple Western Mouse
Zoratti G, Tanabe L, Varela F, Murray A, Bergum C, Colombo E, Lang J, Molinolo A, Leduc R, Marsault E, Boerner J, List K Targeting matriptase in breast cancer abrogates tumour progression via impairment of stromal-epithelial growth factor signalling. Nat Commun, 2015;6(0):6776. 2015 [PMID: 25873032] (Western Blot, Human) Western Blot Human
Sales K, Friis S, Abusleme L, Moutsopoulos N, Bugge T Matriptase promotes inflammatory cell accumulation and progression of established epidermal tumors. Oncogene, 2015;34(35):4664-72. 2015 [PMID: 25486433] (Western Blot, Mouse) Western Blot Mouse
Szabo R, Peters D, Kosa P, Camerer E, Bugge T Regulation of feto-maternal barrier by matriptase- and PAR-2-mediated signaling is required for placental morphogenesis and mouse embryonic survival. PLoS Genet, 2014;10(7):e1004470. 2014 [PMID: 25078604] (Western Blot, Mouse) Western Blot Mouse
Beaulieu A, Gravel E, Cloutier A, Marois I, Colombo E, Desilets A, Verreault C, Leduc R, Marsault E, Richter M Matriptase proteolytically activates influenza virus and promotes multicycle replication in the human airway epithelium. J Virol, 2013;87(8):4237-51. 2013 [PMID: 23365447] (ICC, Human) ICC Human
Szabo , Roman, Uzzun Sales , Katiuchi, Kosa , Peter, Shylo , Natalia, Godiksen , Sine, Hansen , Karina K, Friis , Stine, Gutkind , J Silvio, Vogel , Lotte K, Hummler , Edith, Camerer , Eric, Bugge , Thomas H Reduced prostasin (CAP1/PRSS8) activity eliminates HAI-1 and HAI-2 deficiency-associated developmental defects by preventing matriptase activation. PLoS Genet, 2012;8(8):e1002937. 2012 [PMID: 22952456] (Western Blot, Mouse) Western Blot Mouse
Show All 14 Publications.

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Gene Symbol ST14