Matriptase/ST14 Antibody [Unconjugated]

Images

 
PC‑3 human prostate cancer cell line was stained with Sheep Anti-Human Matriptase/ST14 Catalytic Domain Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF3946, filled histogram) or control antibody (Catalog ...read more
Zymogen-locked matriptase induces epidermal prostasin processing. Protein extracts from skin (a), kidney (b), lung (c), and intestine (d) from newborn St14zym/zym (lanes 1 and 2), St14+/+ (lanes 3 and 4), and ...read more
Zymogen-locked matriptase induces epidermal prostasin processing. Protein extracts from skin (a), kidney (b), lung (c), and intestine (d) from newborn St14zym/zym (lanes 1 and 2), St14+/+ (lanes 3 and 4), and ...read more
Zymogen-locked matriptase induces epidermal prostasin processing. Protein extracts from skin (a), kidney (b), lung (c), and intestine (d) from newborn St14zym/zym (lanes 1 and 2), St14+/+ (lanes 3 and 4), and ...read more
Zymogen-locked matriptase induces epidermal prostasin processing. Protein extracts from skin (a), kidney (b), lung (c), and intestine (d) from newborn St14zym/zym (lanes 1 and 2), St14+/+ (lanes 3 and 4), and ...read more
Zymogen-locked matriptase induces epidermal prostasin processing. Protein extracts from skin (a), kidney (b), lung (c), and intestine (d) from newborn St14zym/zym (lanes 1 and 2), St14+/+ (lanes 3 and 4), and ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, CyTOF-ready, Flow
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

Matriptase/ST14 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human Matriptase/ST14 Catalytic Domain
Val615-Val855
Accession # Q9Y5Y6
Specificity
Detects human Matriptase/ST14 Catalytic Domain in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
ST14
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Intracellular Staining by Flow Cytometry 2.5 ug/10^6 cells
  • Western Blot 0.1 ug/mL
Publications
Read Publications using
AF3946 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Matriptase/ST14 Antibody [Unconjugated]

  • EC 3.4.21
  • Epithin
  • HAI
  • Matriptase
  • Membrane-type serine protease 1
  • MTSP1
  • MT-SP1EC 3.4.21.109
  • prostamin
  • PRSS14
  • Serine protease 14
  • Serine protease TADG-15
  • SNC19
  • SNC19MTSP1
  • ST14
  • suppression of tumorigenicity 14 (colon carcinoma)
  • suppression of tumorigenicity 14 (colon carcinoma, matriptase, epithin)
  • suppressor of tumorigenicity 14 protein
  • TADG15
  • TADG-15
  • TMPRSS14
  • tumor associated differentially expressed gene 15 protein
  • Tumor-associated differentially-expressed gene 15 protein

Background

Human matriptase, encoded by the ST14 (suppression of tumorogenicity 14) gene, is also known as tumor associated differentially expressed gene 15 protein/TADG‑15), epithin, and membrane-type serine protease 1/MT-SP1 (1). Predicted to have a significant role in tumor biology, matriptase may be a novel target for anti-cancer therapy (2). However, expressed in most human epithelia, matriptase is also important in several physiological processes (1). For example, it activates prostasin to initiate a protease cascade that is essential for epidermal differentiation (3), and it converts a single-chain IGFBP-rp1 into the two-chain form (4).

Matriptase is a type II transmembrane serine protease with a complex modular structure (1). The 855 amino acid (aa) sequence of human matriptase consists of a cytoplasmic tail (aa 1-55), a transmembrane domain (aa 56-76), and an extracellular portion (aa 77-855). The latter contains the following domains: SEA (aa 86-201), two CUBs (aa 214-334 and 340-447), four LDLRAs (aa 452-486, 487-523, 524-560, and 566-603), and a serine protease (aa 615-855). The physiological activation of the single-chain zymogen requires the cleavage at the SEA domain within the ER or Golgi, association with HAI-1, which facilitates the transport of the protease to the cell surface, and auto-cleavage at QAR-V(615)VGG (1). The activated matriptase is inhibited by HAI-1, and the resulting HAI-1 complex can be shed from the cell surface (1). R&D Systems rhST14 corresponds to the catalytic domain, and is inhibited effectively by rhHAI-1 and rhHAI-2A (R&D Systems, Catalog # 1048-PI and 1106-PI).

  1. List, K. et al. (2006) Mol. Med. 12:1.
  2. Uhland, K. (2006) Cell. Mol. Life Sci. 63:2968.
  3. Netzel-Arnett, S. et al. (2006) J. Biol. Chem. 281:32941.
  4. Ahmed, S. et al. (2006) FEBS J. 273:615.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Matriptase/ST14 Antibody (AF3946)(19)

We have publications tested in 3 confirmed species: Human, Mouse, Drosophila.

We have publications tested in 5 applications: ICC, IHC, IHC-P, Simple Western, Western Blot.


Filter By Application
ICC
(2)
IHC
(4)
IHC-P
(6)
Simple Western
(1)
Western Blot
(11)
All Applications
Filter By Species
Human
(3)
Mouse
(12)
Drosophila
(1)
All Species
Showing Publications 1 - 10 of 19. Show All 19 Publications.
Publications using AF3946 Applications Species
Ehret, E;Stroh, S;Auberson, M;Ino, F;Jäger, Y;Maillard, M;Szabo, R;Bugge, TH;Frateschi, S;Hummler, E; Kidney-Specific Membrane-Bound Serine Proteases CAP1/Prss8 and CAP3/St14 Affect ENaC Subunit Abundances but Not Its Activity Cells 2023-09-23 [PMID: 37830556] (Western Blot, Mouse) Western Blot Mouse
Li-Mei Chen, Karl X. Chai Exosome-Mediated Activation of the Prostasin-Matriptase Serine Protease Cascade in B Lymphoma Cells Cancers (Basel) 2023-07-28 [PMID: 37568664]
Elaine Zayas Marcelino da Silva, Thais Fernanda de Campos Fraga-Silva, Yao Yuan, Márcia Gaião Alves, Gabriel Azevedo Publio, Carol Kobori da Fonseca et al. Kallikrein 5 Inhibition by the Lympho-Epithelial Kazal-Type Related Inhibitor Hinders Matriptase-Dependent Carcinogenesis Cancers (Basel) 2021-08-31 [PMID: 34503205]
Daniel Essigke, Alexandr V. Ilyaskin, Matthias Wörn, Bernhard N. Bohnert, Mengyun Xiao, Christoph Daniel et al. Zymogen‐locked mutant prostasin (Prss8) leads to incomplete proteolytic activation of the epithelial sodium channel (ENaC) and severely compromises triamterene tolerance in mice Acta Physiol (Oxf) 2021-05-01 [PMID: 33650216]
CJ Wu, M Lu, X Feng, G Nakato, MC Udey Matriptase Cleaves EpCAM and TROP2 in Keratinocytes, Destabilizing Both Proteins and Associated Claudins Cells, 2020-04-21;9(4):. 2020-04-21 [PMID: 32326212] (Western Blot, Human) Western Blot Human
L Drees, T Königsmann, MHJ Jaspers, R Pflanz, D Riedel, R Schuh Conserved function of the matriptase-prostasin proteolytic cascade during epithelial morphogenesis PLoS Genet., 2019-01-02;15(1):e1007882. 2019-01-02 [PMID: 30601807] (IHC, Drosophila) IHC Drosophila
S Friis, D Tadeo, SM Le-Gall, HJ Jürgensen, KU Sales, E Camerer, TH Bugge Matriptase zymogen supports epithelial development, homeostasis and regeneration BMC Biol., 2017-06-01;15(1):46. 2017-06-01 [PMID: 28571576] (IHC, Western Blot, Mouse) IHC, Western Blot Mouse
MS Buzza, TA Johnson, GD Conway, EW Martin, S Mukhopadhy, T Shea-Donoh, TM Antalis Inflammatory cytokines down-regulate the barrier protective prostasin-matriptase proteolytic cascade early in experimental colitis J. Biol. Chem., 2017-05-10;0(0):. 2017-05-10 [PMID: 28490634] (IHC, Mouse) IHC Mouse
Chuan-Jin Wu, Xu Feng, Michael Lu, Sohshi Morimura, Mark C. Udey Matriptase-mediated cleavage of EpCAM destabilizes claudins and dysregulates intestinal epithelial homeostasis Journal of Clinical Investigation 2017-02-01 [PMID: 28094766]
S Friis, DH Madsen, TH Bugge Distinct Developmental Functions of Prostasin (CAP1/PRSS8) Zymogen and Activated Prostasin J. Biol. Chem, 2015-12-30;291(6):2577-82. 2015-12-30 [PMID: 26719335] (Simple Western, Western Blot, Mouse) Simple Western, Western Blot Mouse
Show All 19 Publications.

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Bioinformatics

Gene Symbol ST14
Uniprot