Matriptase/ST14 Antibody

Images

 
PC‑3 human prostate cancer cell line was stained with Sheep Anti-Human Matriptase/ST14 Catalytic Domain Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF3946, filled histogram) or control antibody (Catalog ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, CyTOF-ready, Flow
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
View Available Formulations
Catalog# & Formulation Size Price

Matriptase/ST14 Antibody Summary

Immunogen
E. coli-derived recombinant human Matriptase/ST14 Catalytic Domain
Val615-Val855
Accession # Q9Y5Y6
Specificity
Detects human Matriptase/ST14 Catalytic Domain in direct ELISAs and Western blots. In direct ELISAs, approximately 60% cross-reactivity with recombinant mouse Matriptase/Epithin is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
ST14
Purity
Immunogen affinity purified
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • CyTOF-ready
  • Intracellular Staining by Flow Cytometry 2.5 ug/10^6 cells
Publications
Read Publications using
AF3946 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Matriptase/ST14 Antibody

  • EC 3.4.21
  • Epithin
  • HAI
  • Matriptase
  • Membrane-type serine protease 1
  • MTSP1
  • MT-SP1EC 3.4.21.109
  • Prostamin
  • PRSS14
  • Serine protease 14
  • Serine protease TADG-15
  • SNC19
  • SNC19MTSP1
  • ST14
  • suppression of tumorigenicity 14 (colon carcinoma)
  • suppression of tumorigenicity 14 (colon carcinoma, matriptase, epithin)
  • suppressor of tumorigenicity 14 protein
  • TADG15
  • TADG-15
  • TMPRSS14
  • tumor associated differentially expressed gene 15 protein
  • Tumor-associated differentially-expressed gene 15 protein

Background

Human matriptase, encoded by the ST14 (suppression of tumorogenicity 14) gene, is also known as tumor associated differentially expressed gene 15 protein/TADG‑15), epithin, and membrane-type serine protease 1/MT-SP1 (1). Predicted to have a significant role in tumor biology, matriptase may be a novel target for anti-cancer therapy (2). However, expressed in most human epithelia, matriptase is also important in several physiological processes (1). For example, it activates prostasin to initiate a protease cascade that is essential for epidermal differentiation (3), and it converts a single-chain IGFBP-rp1 into the two-chain form (4).

Matriptase is a type II transmembrane serine protease with a complex modular structure (1). The 855 amino acid (aa) sequence of human matriptase consists of a cytoplasmic tail (aa 1-55), a transmembrane domain (aa 56-76), and an extracellular portion (aa 77-855). The latter contains the following domains: SEA (aa 86-201), two CUBs (aa 214-334 and 340-447), four LDLRAs (aa 452-486, 487-523, 524-560, and 566-603), and a serine protease (aa 615-855). The physiological activation of the single-chain zymogen requires the cleavage at the SEA domain within the ER or Golgi, association with HAI-1, which facilitates the transport of the protease to the cell surface, and auto-cleavage at QAR-V(615)VGG (1). The activated matriptase is inhibited by HAI-1, and the resulting HAI-1 complex can be shed from the cell surface (1). R&D Systems rhST14 corresponds to the catalytic domain, and is inhibited effectively by rhHAI-1 and rhHAI-2A (R&D Systems, Catalog # 1048-PI and 1106-PI).

  1. List, K. et al. (2006) Mol. Med. 12:1.
  2. Uhland, K. (2006) Cell. Mol. Life Sci. 63:2968.
  3. Netzel-Arnett, S. et al. (2006) J. Biol. Chem. 281:32941.
  4. Ahmed, S. et al. (2006) FEBS J. 273:615.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

AF1048
Species: Hu
Applications: WB, Simple Western, IHC
NB100-91761
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
NBP2-47602
Species: Hu
Applications: ICC/IF, IHC, IHC-P
H00005241-M08
Species: Hu, Rt
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
NB100-91761
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
NBP2-34390
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P, Flow-IC
NBP2-34594
Species: Hu, Pm
Applications: Flow, ICC/IF, IHC, IHC-P, CyTOF-ready
AF4478
Species: Hu
Applications: WB, IHC
AF4599
Species: Hu
Applications: WB, Simple Western, IHC, IP
NBP1-69571
Species: Hu, Mu
Applications: WB, IHC, IHC-P
AF3946
Species: Hu
Applications: WB, CyTOF-ready, Flow

Publications for Matriptase/ST14 Antibody (AF3946)(14)

We have publications tested in 3 confirmed species: Human, Mouse, Drosophila.

We have publications tested in 4 applications: ICC, IHC, Simple Western, Western Blot.


Filter By Application
ICC
(1)
IHC
(3)
Simple Western
(1)
Western Blot
(9)
All Applications
Filter By Species
Human
(3)
Mouse
(10)
Drosophila
(1)
All Species
Showing Publications 1 - 10 of 14. Show All 14 Publications.
Publications using AF3946 Applications Species
CJ Wu, M Lu, X Feng, G Nakato, MC Udey Matriptase Cleaves EpCAM and TROP2 in Keratinocytes, Destabilizing Both Proteins and Associated Claudins Cells, 2020;9(4):. 2020 [PMID: 32326212] (Western Blot, Human) Western Blot Human
L Drees, T Königsmann, MHJ Jaspers, R Pflanz, D Riedel, R Schuh Conserved function of the matriptase-prostasin proteolytic cascade during epithelial morphogenesis PLoS Genet., 2019;15(1):e1007882. 2019 [PMID: 30601807] (IHC, Drosophila) IHC Drosophila
S Friis, D Tadeo, SM Le-Gall, HJ Jürgensen, KU Sales, E Camerer, TH Bugge Matriptase zymogen supports epithelial development, homeostasis and regeneration BMC Biol., 2017;15(1):46. 2017 [PMID: 28571576] (Western Blot, Mouse) Western Blot Mouse
MS Buzza, TA Johnson, GD Conway, EW Martin, S Mukhopadhy, T Shea-Donoh, TM Antalis Inflammatory cytokines down-regulate the barrier protective prostasin-matriptase proteolytic cascade early in experimental colitis J. Biol. Chem., 2017;0(0):. 2017 [PMID: 28490634] (IHC, Mouse) IHC Mouse
S Friis, DH Madsen, TH Bugge Distinct Developmental Functions of Prostasin (CAP1/PRSS8) Zymogen and Activated Prostasin J. Biol. Chem, 2016;291(6):2577-82. 2016 [PMID: 26719335] (Simple Western, Mouse) Simple Western Mouse
Zoratti G, Tanabe L, Varela F, Murray A, Bergum C, Colombo E, Lang J, Molinolo A, Leduc R, Marsault E, Boerner J, List K Targeting matriptase in breast cancer abrogates tumour progression via impairment of stromal-epithelial growth factor signalling. Nat Commun, 2015;6(0):6776. 2015 [PMID: 25873032] (Western Blot, Human) Western Blot Human
Sales K, Friis S, Abusleme L, Moutsopoulos N, Bugge T Matriptase promotes inflammatory cell accumulation and progression of established epidermal tumors. Oncogene, 2015;34(35):4664-72. 2015 [PMID: 25486433] (Western Blot, Mouse) Western Blot Mouse
Szabo R, Peters D, Kosa P, Camerer E, Bugge T Regulation of feto-maternal barrier by matriptase- and PAR-2-mediated signaling is required for placental morphogenesis and mouse embryonic survival. PLoS Genet, 2014;10(7):e1004470. 2014 [PMID: 25078604] (Western Blot, Mouse) Western Blot Mouse
Beaulieu A, Gravel E, Cloutier A, Marois I, Colombo E, Desilets A, Verreault C, Leduc R, Marsault E, Richter M Matriptase proteolytically activates influenza virus and promotes multicycle replication in the human airway epithelium. J Virol, 2013;87(8):4237-51. 2013 [PMID: 23365447] (ICC, Human) ICC Human
Szabo, Roman, Uzzun Sales, Katiuchi, Kosa, Peter, Shylo, Natalia, Godiksen, Sine, Hansen, Karina K, Friis, Stine, Gutkind, J Silvio, Vogel, Lotte K, Hummler, Edith, Camerer, Eric, Bugge, Thomas H Reduced prostasin (CAP1/PRSS8) activity eliminates HAI-1 and HAI-2 deficiency-associated developmental defects by preventing matriptase activation. PLoS Genet, 2012;8(8):e1002937. 2012 [PMID: 22952456] (Western Blot, Mouse) Western Blot Mouse
Show All 14 Publications.

Reviews for Matriptase/ST14 Antibody (AF3946) (0)

There are no reviews for Matriptase/ST14 Antibody (AF3946). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Matriptase/ST14 Antibody (AF3946) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin BAF3946

Additional Matriptase/ST14 Products

Bioinformatics Tool for Matriptase/ST14 Antibody (AF3946)

Discover related pathways, diseases and genes to Matriptase/ST14 Antibody (AF3946). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Matriptase/ST14 Antibody (AF3946)

Discover more about diseases related to Matriptase/ST14 Antibody (AF3946).
 

Pathways for Matriptase/ST14 Antibody (AF3946)

View related products by pathway.

PTMs for Matriptase/ST14 Antibody (AF3946)

Learn more about PTMs related to Matriptase/ST14 Antibody (AF3946).

Blogs on Matriptase/ST14

There are no specific blogs for Matriptase/ST14, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Matriptase/ST14 Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol ST14
Uniprot