Mouse myeloma cell line NS0-derived recombinant human HAI-1 Pro37-Glu449 Accession # NP_003701
Detects human HAI-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant mouse HAI‑1 is observed and less than 1% cross-reactivity with recombinant human HAI-2 is observed.
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Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Reconstitute at 0.2 mg/mL in sterile PBS.
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for HAI-1/HGFA Inhibitor 1 Antibody [Unconjugated]
hepatocyte growth factor activator inhibitor 1
Hepatocyte growth factor activator inhibitor type 1
kunitz-type protease inhibitor 1
serine peptidase inhibitor, Kunitz type 1
serine protease inhibitor, Kunitz type 1
HAI-1 is a Kunitz-type serine protease inhibitor, identified as a strong inhibitor of HGF activator (HGFA) and matriptase (1). The membrane-anchored HAI-1 consists of two Kunitz domains, a LDL-receptor-like domain, and a C-terminal transmembrane domain (2). Two soluble forms are generated by ectodomain shedding, one with a single Kunitz domain and the other with two Kunitz domains. HAI-1 is not only an inhibitor but also a specific receptor of active HGFA, acting as a reservoir of this enzyme on the cell surface (3). The shedding of HAI-1 and HGFA/HAI-1 complex is enhanced by treatment with phorbol 12-myristate 13-acetate or IL-1 beta. The regulated shedding is completely inhibited by a synthetic zinc metalloprotease inhibitor (3).
Denda, K. et al. (2002) J. Biol. Chem. 277:14053.
Shimomura, T. et al. (1997) J. Biol. Chem. 272:6370.
Kataoka, H. et al. (2000) J. Biol. Chem. 275:40453.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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