Heparan Sulfate Proteoglycan 2 Antibody (A74) - BSA Free

• Reactivity: Hu, Bv
• Applications: WB, Simple Western, ELISA, IHC, IP, AP
• Clone: A74
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Heparan Sulfate Proteoglycan 2 Antibody (A74) - BSA Free Summary

• Description: Novus Biologicals Mouse Heparan Sulfate Proteoglycan 2 Antibody (A74) - BSA Free (NBP2-23523) is a monoclonal antibody validated for use in IHC, WB, ELISA, Simple Western and IP. Anti-Heparan Sulfate Proteoglycan 2 Antibody: Cited in 3 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Bovine corneal endothelial cells
• Epitope: Epitope is located in domain V
• Specificity: Highly specific for perlecan. There is no evidence for cross-reactivity with other connective tissue proteins (vitronectin, fibronectin, elastin, collagen, laminin).
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: HSPG2
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunoaffinity Purification
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation 1:10-1:500
  • Simple Western 1:500
  • Western Blot 1:100-1:2000
• Application Notes: ELISA: NBP2-23523 can be used in ELISA. WB: In Western blotting a dilution guideline of 1/100 has proved successful. (1) IHC: NBP2-23523 can be used in immunostaining of frozen PLP-fixed sections of bovine and human tissues. AP: NBP2-23523 can be used in affinity chromatography, in conjunction with NBP2-23522 to separate recombinant domain I from full length perlecan. (1) IP: NBP2-23523 can be used in immunoprecipitation.
  See Simple Western Antibody Database for Simple Western validation: Tested in Fibroblasts, separated by Size, antibody dilution of 1:500

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 10mM Phosphate (pH 7.4) and 0.5M NaCl
• Preservative: 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A or G purified

Alternate Names for Heparan Sulfate Proteoglycan 2 Antibody (A74) - BSA Free

• basement membrane-specific heparan sulfate proteoglycan core protein
• endorepellin (domain V region)
• heparan sulfate proteoglycan 2
• HSPG
• perlecan proteoglycan
• Perlecan
• PLCSchwartz-Jampel syndrome 1 (chondrodystrophic myotonia)
• PRCAN
• SJA
• SJS
• SJS1

Background

Perlecan is an extracellular matrix proteoglycan. It has a large core protein of 400-450 kDa and is often produced with heparan sulfate side chains. Perlecan is found in basement membranes where it contributes to the permeability characteristics, serves as a substrate for vascular cells and binds growth factors involved in vascular remodelling .

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Heparan Sulfate Proteoglycan 2 Antibody (NBP2-23523)(3)

Publications using NBP2-23523

• Wi?niewska K, Rintz E, ?abi?ska M et al. Comprehensive evaluation of pathogenic protein accumulation in fibroblasts from all subtypes of Sanfilippo disease patients. Biochemical and biophysical research communications 2024-09-19 [PMID: 39305572]
• Gaffke L, Firyn N, Rintz E et al. Therapeutic potential of lithium chloride and valproic acid against neuronopathic types of mucopolysaccharidoses through induction of the autophagy process Archives of biochemistry and biophysics 2023-09-12 [PMID: 37708928]
• Rintz E, Podlacha M, Cyske Z et al. Activities of (Poly)phenolic Antioxidants and Other Natural Autophagy Modulators in the Treatment of Sanfilippo Disease: Remarkable Efficacy of Resveratrol in Cellular and Animal Models Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics 2022-11-07 [PMID: 36344724] (Cytometric Bead Assay Standard, Human)

Bioinformatics

• Gene Symbol: HSPG2