Heparan Sulfate 6-O-Sulfotransferase 2/HS6ST2 Antibody

• Reactivity: Hu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Heparan Sulfate 6-O-Sulfotransferase 2/HS6ST2 Antibody Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Heparan Sulfate 6-O-Sulfotransferase 2/HS6ST2
  Gln179-Arg605
  Accession # Q96MM7
• Specificity: Detects human Heparan Sulfate 6-O-Sulfotransferase 2/HS6ST2 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: HS6ST2
• Purity: Immunogen affinity purified
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 25 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Heparan Sulfate 6-O-Sulfotransferase 2/HS6ST2 Antibody

• EC-1
• heparan sulfate 6-O-sulfotransferase 2
• HS6ST2

Background

HS6ST2 is a key enzyme in HS biosynthesis. There are at least three alternatively spliced forms, which include a long form (645 aa, NP_001070656), an intermediate form (605 aa, Q96MM7) with 40 aa deleted between Arg 316 and Asn 357 of the long form, and a short form starting at Met 147 of the intermediate form. Both the long and intermediate forms predict a type II membrane protein, consistent with its Golgi location. The long form of human HS6ST2 shares 94% and 86% aa sequence identities with that of chimpanzee, dog, mouse, rat, and chicken, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Heparan Sulfate 6-O-Sulfotransferase 2/HS6ST2 Antibody (AF2710)(1)

Publication using AF2710

• BE Thacker, KJ Thorne, C Cartwright, J Park, K Glass, A Chea, BP Kellman, NE Lewis, Z Wang, A Di Nardo, ST Sharfstein, W Jeske, J Walenga, J Hogwood, E Gray, B Mulloy, JD Esko, CA Glass Multiplex genome editing of mammalian cells for producing recombinant heparin Metabolic engineering, 2022;0(0):. 2022 [PMID: 35038554] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: HS6ST2
• Uniprot:
  • Q96MM7()