HDHD1A Antibody (OTI7A2)

Western Blot: HDHD1A Antibody (7A2) [NBP2-00487] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HDHD1A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HDHD1A.

Immunohistochemistry-Paraffin: HDHD1A Antibody (7A2) [NBP2-00487] - Staining of paraffin-embedded Human prostate tissue using anti-HDHD1A mouse monoclonal antibody.

Flow Cytometry: HDHD1A Antibody (7A2) [NBP2-00487] - Analysis of Jurkat cells, using anti-HDHD1A antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Western Blot: HDHD1A Antibody (7A2) [NBP2-00487] Analysis of extracts (35ug) from 9 different cell lines by using anti-HDHD1A monoclonal antibody.

Immunohistochemistry-Paraffin: HDHD1A Antibody (7A2) [NBP2-00487] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-HDHD1A mouse monoclonal antibody.

Immunohistochemistry-Paraffin: HDHD1A Antibody (7A2) [NBP2-00487] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-HDHD1A mouse monoclonal antibody.

Immunohistochemistry-Paraffin: HDHD1A Antibody (7A2) [NBP2-00487] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-HDHD1A mouse monoclonal antibody.

Immunohistochemistry-Paraffin: HDHD1A Antibody (7A2) [NBP2-00487] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-HDHD1A mouse monoclonal antibody.

Immunohistochemistry-Paraffin: HDHD1A Antibody (7A2) [NBP2-00487] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-HDHD1A mouse monoclonal antibody.

Immunohistochemistry-Paraffin: HDHD1A Antibody (7A2) [NBP2-00487] - Staining of paraffin-embedded Human breast tissue using anti-HDHD1A mouse monoclonal antibody.

Immunohistochemistry-Paraffin: HDHD1A Antibody (7A2) [NBP2-00487] - Staining of paraffin-embedded Human pancreas tissue using anti-HDHD1A mouse monoclonal antibody.

Flow Cytometry: HDHD1A Antibody (7A2) [NBP2-00487] - Analysis of Hela cells, using anti-HDHD1A antibody, (Red), compared to a nonspecific negative control antibody (Blue).

• Reactivity: Hu, Mu, Rt, Ca, Pm, Pm
• Applications: WB, Flow, IHC
• Clone: OTI7A2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1.2 mg/ml

HDHD1A Antibody (OTI7A2) Summary

• Description: Novus Biologicals Mouse HDHD1A Antibody (OTI7A2) (NBP2-00487) is a monoclonal antibody validated for use in IHC, WB and Flow. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human HDHD1(NP_036212) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PUDP
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:500-2000
• Theoretical MW: 23.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for HDHD1A Antibody (OTI7A2)

• DXF68S1E5'-PsiMPase
• EC 3.1.3.n6
• family with sequence similarity 16, member A, X-linked
• GS1FAM16AX
• haloacid dehalogenase-like hydrolase domain containing 1
• haloacid dehalogenase-like hydrolase domain containing 1A
• Haloacid dehalogenase-like hydrolase domain-containing protein 1
• Haloacid dehalogenase-like hydrolase domain-containing protein 1A
• HDHD1Apseudouridine-5'-monophosphatase
• Protein GS1

Background

This gene encodes a member of the haloacid dehalogenase-like (HAD) hydrolase superfamily. The encoded protein has no known biological function. This gene has a pseudogene on chromosome 1. Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PUDP
• Entrez:
  • 8226(Human)