HDAC9 Antibody - N-terminal

• Reactivity: Hu
• Applications: IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

HDAC9 Antibody - N-terminal Summary

• Immunogen: A synthetic peptide at the N-terminal of human HDAC9 protein.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HDAC9
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
• Application Notes: This antibody is tested by Immunohistochemistry and useful for ELISA and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~111kDa corresponding to the appropriate cell lysate or extract.

Reactivity Notes

BLAST analysis was used to suggest cross-reactivity with human based on 100% sequence homology. Cross-reactivity with HDAC9 from other sources has not been determined.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 30% Glycerol
• Preservative: No Preservative
• Purity: Immunogen affinity purified

Alternate Names for HDAC9 Antibody - N-terminal

• EC 3.5.1.98
• HD7b
• HD7HDAC
• HD9
• HDAC7
• HDAC7B
• HDAC7BDKFZp779K1053
• HDAC9
• HDRP
• histone deacetylase 4/5-related protein
• Histone deacetylase 7B
• histone deacetylase 9
• Histone deacetylase-related protein
• KIAA0744
• KIAA0744HDAC9B
• MEF-2 interacting transcription repressor (MITR) protein
• MEF2-interacting transcription repressor MITR
• MITR
• MITRHDAC9FL

Background

In the intact cell, DNA closely associates with histones and other nuclear proteins to form chromatin. The remodeling of chromatin is believed to be a critical component of transcriptional regulation and a major source of this remodeling is brought about by the acetylation of nucleosomal histones. Acetylation of lysine residues in the amino terminal tail domain of histone results in an allosteric change in the nucleosomal conformation and an increased accessibility to transcription factors by DNA. Conversely, the deacetylation of histones is associated with transcriptional silencing. Several mammalian proteins have been identified as nuclear histone acetylases, including GCN5, PCAF (p300/CBP-associated factor), p300/CBP, HAT1 and the TFIID subunit TAF II p250. Mammalian HDAC8, isolated from human kidney, is a histone deacetylase that shares homology to other HDACs but has different tissue distribution. HDAC8 is localized to the nucleus and plays a role in the development of a broad range of tissues and in the etiology of cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HDAC9