HDAC6 Antibody

Western Blot: HDAC6 Antibody [NBP1-45611] - Analysis of HDAC-6 expression with Jurkat cell lysate.

Senescent intestinal cells reorganize microtubules.a Microtubule stability was evaluated with nocodazole for IECs treated with vehicle (V), sodium butyrate (NaBu), or lopinavir. Disassembly or partial disassembly was scored10. Data were normalized to vehicle controls. Average + SEM are shown for three independent experiments. For each experiment, 100–157 cells were examined for every time point and condition. b Immunocytochemistry revealed changes in the abundance and organization of alpha -tubulin acetylated on K40 and total alpha -tubulin (Tubulin). DAPI stained nuclei; scale bar is 20 um. Graphs depict pixel intensities/cells normalized to vehicle controls (V). At least 135 cells were assessed for each condition. Bars represent average + SEM. c Semiquantitative Western blotting determined the abundance of alpha -tubulin acetylated on K40 (Ac-K40-Tubulin) and total alpha -tubulin. Actin was used as a loading control. Between three and seven independent experiments were evaluated by Western blotting. d Western blotting determined the abundance of HDAC6, Rock1, and Rock2 for the conditions depicted. Graphs represent averages + SEM for 3–8 independent experiments. a, b Student’s t-test evaluated differences between control and senescent cells; *p < 0.05; **p < 0.01; ***p < 0.001. c, d The molecular masses of marker proteins in kDa are indicated at the right margin of blots. One-way ANOVA combined with Bonferroni correction identified significant differences between the vehicle control and treated cells. *p < 0.05; **p < 0.01; ***p < 0.001. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36526654), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

HDAC6 Antibody Summary

• Description: This product is manufactured by Abcam and distributed by Novus Biologicals. (Abcam Catalog Number: ab1440)
• Immunogen: Synthetic peptide mapping to the N-terminus of mouse HDAC-6.
• Specificity: The antibody does not cross-react with other HDAC proteins including HDAC1, 2, 3, 4, 5, 7, and 8.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HDAC6
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 20 ug/mL
  • Western Blot 0.5 - 4 ug/mL
• Theoretical MW: 131 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2), 5 mM EDTA, 0.5% BSA, 30% Glycerol
• Preservative: 0.01% Thimerosal
• Concentration: 0.5 mg/ml
• Purity: Affinity purified

Alternate Names for HDAC6 Antibody

• CPBHM
• EC 3.5.1.98
• FLJ16239
• HD6
• HD6FLJ16239
• HDAC6
• Histone Deacetylase 6
• JM21
• KIAA0901
• PPP1R90

Background

In the intact cell, DNA closely associates with histones and other nuclear proteins to form chromatin. The remodeling of chromatin is believed to be a critical component of transcriptional regulation and a major source of this remodeling is brought about by the acetylation of nucleosomal histones. Acetylation of lysine residues in the amino terminal tail domain of histone results in an allosteric change in the nucleosomal conformation and an increased accessibility to transcription factors by DNA. Conversely, the deacetylation of histones is associated with transcriptional silencing. Several mammalian proteins have been identified as nuclear histone acetylases, including GCN5, PCAF (p300/CBP-associated factor), p300/CBP, HAT1 and the TFIID subunit TAF II p250. Mammalian HDAC1 (also designated HD1), HDAC2 (also designated RPD3) and HDAC3-6 have been identified as histone deacetylases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

&#9888; WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for HDAC6 Antibody (NBP1-45611)(1)

Publication using NBP1-45611

• Sun K, Zhang H, Zhang T et al. Spinal HDAC6 mediates nociceptive behaviors induced by CCI via neuronal activation and neuroinflammation Molecular pain 2023-11-20 [PMID: 37982151] (WB, Mouse)

Bioinformatics

• Gene Symbol: HDAC6
• Entrez:
  • 10013(Human)
  • 15185(Mouse)
• Uniprot:
  • Q9Z2V5(Mouse)