HDAC6 Antibody

Western Blot: HDAC6 Antibody [NBP1-45611] - Analysis of HDAC-6 expression with Jurkat cell lysate.

Product Discontinued

HDAC6 Antibody Summary

• Description: This product is manufactured by Abcam and distributed by Novus Biologicals. (Abcam Catalog Number: ab1440)
• Immunogen: Synthetic peptide mapping to the N-terminus of mouse HDAC-6.
• Specificity: The antibody does not cross-react with other HDAC proteins including HDAC1, 2, 3, 4, 5, 7, and 8.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HDAC6
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 20 ug/mL
  • Western Blot 0.5 - 4 ug/mL
• Theoretical MW: 131 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2), 5 mM EDTA, 0.5% BSA, 30% Glycerol
• Preservative: 0.01% Thimerosal
• Concentration: 0.5 mg/ml
• Purity: Affinity purified

Alternate Names for HDAC6 Antibody

• CPBHM
• EC 3.5.1.98
• FLJ16239
• HD6
• HD6FLJ16239
• HDAC6
• Histone Deacetylase 6
• JM21
• KIAA0901
• PPP1R90

Background

In the intact cell, DNA closely associates with histones and other nuclear proteins to form chromatin. The remodeling of chromatin is believed to be a critical component of transcriptional regulation and a major source of this remodeling is brought about by the acetylation of nucleosomal histones. Acetylation of lysine residues in the amino terminal tail domain of histone results in an allosteric change in the nucleosomal conformation and an increased accessibility to transcription factors by DNA. Conversely, the deacetylation of histones is associated with transcriptional silencing. Several mammalian proteins have been identified as nuclear histone acetylases, including GCN5, PCAF (p300/CBP-associated factor), p300/CBP, HAT1 and the TFIID subunit TAF II p250. Mammalian HDAC1 (also designated HD1), HDAC2 (also designated RPD3) and HDAC3-6 have been identified as histone deacetylases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for HDAC6 Antibody (NBP1-45611)(1)

Publication using NBP1-45611

• Sun K, Zhang H, Zhang T et al. Spinal HDAC6 mediates nociceptive behaviors induced by CCI via neuronal activation and neuroinflammation Molecular pain 2023-11-20 [PMID: 37982151] (WB, Mouse)

Bioinformatics

• Gene Symbol: HDAC6
• Entrez:
  • 10013(Human)
  • 15185(Mouse)
• Uniprot:
  • Q9Z2V5(Mouse)