HDAC6 Antibody

Images

 
Biological Strategies: Western Blot: HDAC6 Antibody [NB100-61065] - Detection of Human HDAC6 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. ...read more
Independent Antibodies: Western Blot: HDAC6 Antibody [NB100-61065] - Detection of Human HDAC6 on HeLa whole cell lysate using NB100-61065. HDAC6 was also immunoprecipitated by rabbit anti-HDAC6 antibody ...read more
Biological Strategies: Western Blot: HDAC6 Antibody [NB100-61065] - Whole cell lysate (50 ug) from TCMK-1 and NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-HDAC6 antibody ...read more
Immunoprecipitation: HDAC6 Antibody [NB100-61065] - Detection of human HDAC6 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

HDAC6 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 1165 and 1215 of human histone deacetylase 6 using the numbering given in entry NP_006035.2 (GeneID 10013).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HDAC6
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunoprecipitation 2 - 10 ug/mg lysate
Control
HDAC6 Overexpression Lysate
HDAC6 Knockout HeLa Cell Lysate

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for HDAC6 Antibody

  • EC 3.5.1.98
  • HD6FLJ16239
  • histone deacetylase 6
  • JM21
  • KIAA0901

Background

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class II of the histone deacetylase/acuc/apha family. It contains an internal duplication of two catalytic domains which appear to function independently of each other. This protein possesses histone deacetylase activity and represses transcription.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

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LAMP2: Protector of the lysosome
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Bioinformatics

Gene Symbol HDAC6
Entrez
Uniprot