HDAC3 Antibody

Images

 
Western Blot: HDAC3 Antibody [NB100-79798] - A) Whole cell lysate (WCL) from HeLa (5, 15 and 50 mcg), Ramos (R; 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. B) WCL (1 mg for IP; 1/4 of IP reaction/gel) from HeLa cells. ...read more
Immunohistochemistry: HDAC3 Antibody [NB100-79798] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-HDAC3 used at a dilution of 1:200 (1ug/ml). Detection: DAB
Immunohistochemistry-Paraffin: HDAC3 Antibody [NB100-79798] - FFPE section of human non-small cell lung cancer. Affinity purified rabbit anti-HDAC3 at a dilution of 1:250.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

HDAC3 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 375 and the C-terminus (residue 428) of human Histone Deacetylase 3 using the numbering given in Swiss-Prot entry O15379 (GeneID 8841).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HDAC3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:5000-1:15000
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Immunoprecipitation 2-5 ug/mg
Application Notes
Epitope retrieval with Tris-EDTA pH9.0 is recommended for FFPE tissue sections.
Positive Control
HDAC3 Lysate (NBL1-11481)
Publications
Read Publications using NB100-79798.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for HDAC3 Antibody

  • EC 3.5.1.98
  • HD3RPD3-2RPD3
  • histone deacetylase 3
  • SMAP45

Background

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family. It has histone deacetylase activity and represses transcription when tethered to a promoter. It may participate in the regulation of transcription through its binding with the zinc-finger transcription factor YY1. This protein can also down-regulate p53 function and thus modulate cell growth and apoptosis. This gene is regarded as a potential tumor suppressor gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for HDAC3 Antibody (NB100-79798)(3)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol HDAC3
Entrez
Uniprot