HDAC10 Antibody (SD08-11)

Western Blot: HDAC10 Antibody (SD08-11) [NBP2-67801] - Analysis of HDAC10 on HepG2 cells lysates using anti-HDAC10 antibody at 1/1,000 dilution.

Immunocytochemistry/Immunofluorescence: HDAC10 Antibody (SD08-11) [NBP2-67801] - Staining HDAC10 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: HDAC10 Antibody (SD08-11) [NBP2-67801] - Immunohistochemical analysis of paraffin-embedded Human placenta tissue using anti-HDAC10 antibody. Counter stained with hematoxylin.

Immunocytochemistry/Immunofluorescence: HDAC10 Antibody (SD08-11) [NBP2-67801] - Staining HDAC10 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: HDAC10 Antibody (SD08-11) [NBP2-67801] - Staining HDAC10 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Product Discontinued

HDAC10 Antibody (SD08-11) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human HDAC10 aa 339-388 / 669. (SwissProt: Q969S8 Human; SwissProt: Q6P3E7 Mouse)
• Localization: Cytoplasm, Nucleus.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: HDAC10
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Western Blot 1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for HDAC10 Antibody (SD08-11)

• DKFZP761B039
• EC 3.5.1.48
• EC 3.5.1.62
• EC 3.5.1.98
• HD10
• HDAC10
• histone deacetylase 10
• MGC149722

Background

In the intact cell, DNA closely associates with histones and other nuclear proteins to form chromatin. The remodeling of chromatin is believed to be a critical component of transcriptional regulation and a major source of this remodeling is brought about by the acetylation of nucleosomal histones. Acetylation of lysine residues in the amino-terminal tail domain of histone results in an allosteric change in the nucleosomal conformation and an increased accessibility to transcription factors by DNA. Conversely, the deacetylation of histones is associated with transcriptional silencing. Several mammalian proteins have been identified as nuclear histone acetylases, including GCN5, PCAF (for p300/CBP-associated factor), p300/CBP and the TFIID subunit TAF II p250. Mammalian HDAC1 (also designated HD1), HDAC2 (also designated mammalian RPD3) and HDAC3, all of which are related to the yeast transcriptional regulator Rpd3p, have been identified as histone deacetylases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HDAC10