HDAC1 Antibody

Images

 
Western Blot: HDAC1 Antibody [NBP1-78101] - Lane 1: 293 whole cell lysate. Lane 2: none. Load: 35 ug per lane. Primary antibody: HDAC-1 antibody at 1:3,500 for overnight at 4C. Secondary antibody: IRDye800 (TM) rabbit ...read more
Immunocytochemistry/ Immunofluorescence: HDAC1 Antibody [NBP1-78101] - Rabbit IgG 611-151-122 (shown in red) for to detect HDAC-1 by immunofluorescence. Anti Keratin monoclonal antibody was used with Dylight 488 goat ...read more
Immunohistochemistry: HDAC1 Antibody [NBP1-78101] - Analysis of Tissue: human prostate cancer tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: HDAC-1 antibody at ...read more
Western Blot: HDAC1 Antibody [NBP1-78101] - Analysis of LNCaP prostate cancer cells. Lysate was loaded at 50ug per lane and incubated with 1:1000 dilution of primary Ab. A band was detected at the expected molecular ...read more
Immunohistochemistry-Paraffin: HDAC1 Antibody [NBP1-78101] - HDAC1 antibody shows strong nuclear staining of tumor cells in human lung tissue. Brown color indicates presence of protein, blue color shows cell nuclei.

Product Details

Summary
Reactivity Hu, Mu, Rt, OrSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.33 mg/ml

Order Details

HDAC1 Antibody Summary

Immunogen
Anti-HDAC1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 466-482 of Human HDAC-1.
Specificity
A BLAST analysis was used to suggest reactivity with this protein from human, mouse, rat and chimpanzee sources based on 100% homology for the immunogen sequence. Cross reactivity may occur with HDAC-1 from bovine (82% homology) and chicken (80% homology) sources. Cross reactivity with HDAC-1 homologues from other sources has not been determined.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HDAC1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:5000
  • ELISA 1:10000-1:50000
  • Immunocytochemistry/Immunofluorescence 10 ug / ml
  • Immunohistochemistry 1:200-1:1000
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
Specific nuclear staining is observed by IHC. Expect bands at 65 kDa in size corresponding - HDAC-1 by western blotting in the appropriate cell lysate or extract.
Control
HDAC1 Knockout HeLa Cell Lysate
Publications
Read Publication using
NBP1-78101 in the following applications:

Reactivity Notes

Chimpanzee 100%, Bovine 82%, Chicken 80%

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
1.33 mg/ml
Purity
Immunogen affinity purified

Alternate Names for HDAC1 Antibody

  • EC 3.5.1.98
  • GON-10
  • HD1
  • HD1DKFZp686H12203
  • Histone Deacetylase 1
  • KDAC1
  • reduced potassium dependency, yeast homolog-like 1
  • RPD3L1
  • RPD3L1RPD3

Background

HDAC1 (also known as HD1, histone deacetylase 1, RPD3, RPD3L1) belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, it deacetylates p53 and modulates its effect on cell growth and apoptosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for HDAC1 Antibody (NBP1-78101)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: IHC-P.


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IHC-P
(1)
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Human
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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for HDAC1 Antibody (NBP1-78101) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Blogs on HDAC1.

The role of DNMT3B in the co-incidence of methyltransferase and tumor suppressor expression in malignancies
Epigenetics is the process of heritable change in gene activity despite alteration of the hosts DNA sequence, essentially causing a change in a phenotype without a change in the genotype of a host. To change the gene sequence without interfering w...  Read full blog post.

The role of DNMT3A in development
Epigenetics is the study of heritable change in gene activity despite alteration of the hosts DNA sequence.  Change in gene activity done independently of the DNA sequence is achieved by way of histone and DNA methylation.  Gene silencing in DNA ...  Read full blog post.

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Bioinformatics

Gene Symbol HDAC1