HAPLN1 Antibody (SY02-17)

Western Blot: HAPLN1 Antibody (SY02-17) [NBP2-67447] - Analysis of HAPLN1 on different lysates using anti-HAPLN1 antibody at 1/1,000 dilution. Positive control: Lane 1: BT-20 Lane 2: HUVEC Lane 3: SW480

Immunocytochemistry/Immunofluorescence: HAPLN1 Antibody (SY02-17) [NBP2-67447] - Staining HAPLN1 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: HAPLN1 Antibody (SY02-17) [NBP2-67447] - Analysis of paraffin-embedded mouse spleen tissue using anti-HAPLN1 antibody. Counter stained with hematoxylin.

Immunocytochemistry/Immunofluorescence: HAPLN1 Antibody (SY02-17) [NBP2-67447] - Staining HAPLN1 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: HAPLN1 Antibody (SY02-17) [NBP2-67447] - Staining HAPLN1 in BT-20 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: HAPLN1 Antibody (SY02-17) [NBP2-67447] - Analysis of paraffin-embedded human liver tissue using anti-HAPLN1 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: HAPLN1 Antibody (SY02-17) [NBP2-67447] - Analysis of paraffin-embedded human spleen tissue using anti-HAPLN1 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: HAPLN1 Antibody (SY02-17) [NBP2-67447] - Analysis of paraffin-embedded human tonsil tissue using anti-HAPLN1 antibody. Counter stained with hematoxylin.

Product Discontinued

HAPLN1 Antibody (SY02-17) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide withinl Human HAPLN1 aa 305-354 / 354. (SwissProt: P10915 Human; SwissProt: Q9QUP5 Mouse; SwissProt: P03994 Rat)
• Localization: Secreted.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: HAPLN1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:100
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for HAPLN1 Antibody (SY02-17)

• Cartilage link protein
• Cartilage-link protein
• Cartilage-linking protein 1
• CRTL1
• CRTL1cartilage linking protein 1
• HAPLN1
• hyaluronan and proteoglycan link protein 1
• Proteoglycan link protein

Background

Th extracellular matrix of cartilage consists of a number of interacting macromolecules that are unique to that tissue. The most studied group of these molecules are those that make up the ternary complex involving proteoglycan monomer, link protein (LP) , and hyaluronic acid (HA). As many as 100 proteoglycan monomers and LPs can be found to interact with a single HA polymer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HAPLN1