HAPLN4 Antibody

HAPLN4 was detected in immersion fixed paraffin-embedded sections of human brain (caudate nucleus) using 3 µg/mL Goat Anti-Human HAPLN4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4085) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

HAPLN4 Antibody Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human HAPLN4
  Gln30-Val402
  Accession # Q86UW8
• Specificity: Detects human HAPLN4 in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human HAPLN1 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: HAPLN4
• Purity: Immunogen affinity purified
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Immunoprecipitation 25 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for HAPLN4 Antibody

• BRAL2
• BRAL2KIAA1926Brain link protein 2
• HAPLN4
• hyaluronan and proteoglycan link protein 4
• Lpr4

Background

HAPLN4 (hyaluronan and proteoglycan link protein 4; also Bral2) is a 40-42 kDa secreted member of the Link module superfamily. It is secreted by select neurons, particularly of the cerebellum, where it participates in ECM stability. Here it may link hyaluronic acid with resident proteoglycans. Mature HAPLN4 is 383 amino acids (aa) in length. It contains one lg-like C2-type domain (aa 46-161) and two link domains (aa 163-365). One potential alternate start site exists 36 aa upstream of the standard start site. Human and mouse HAPLN4 share 91% aa sequence identity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HAPLN4 Antibody (AF4085)(1)

Publication using AF4085

• M Sonntag, M Blosa, S Schmidt, K Reimann, K Blum, T Eckrich, G Seeger, D Hecker, B Schick, T Arendt, J Engel, M Morawski Synaptic coupling of inner ear sensory cells is controlled by brevican-based extracellular matrix baskets resembling perineuronal nets BMC Biol., 2018;16(1):99. 2018 [PMID: 30253762] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: HAPLN4
• Uniprot:
  • Q86UW8()