HAPLN1 Antibody (316119)

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clone
316119
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

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HAPLN1 Antibody (316119) Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human HAPLN1
Asp16-Asn354
Accession # P10915
Specificity
Detects human HAPLN1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody does not cross-react with recombinant human HAPLN4.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
HAPLN1
Purity
Protein A or G purified from hybridoma culture supernatant
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
Reviewed Applications
Read 1 Review rated 5
using
MAB2608 in the following application:

Publications
Read Publication using
MAB2608 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Protein A or G purified from hybridoma culture supernatant
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for HAPLN1 Antibody (316119)

  • Cartilage link protein
  • Cartilage-link protein
  • Cartilage-linking protein 1
  • CRTL1
  • CRTL1cartilage linking protein 1
  • HAPLN1
  • hyaluronan and proteoglycan link protein 1
  • Proteoglycan link protein

Background

HAPLN1 (also known as link protein and CRTL1) is a member of the hyaladherin family of hyaluronic acid (HA) binding proteins. Hyaluronan binding proteins are of two types; those with link modules, and those without. Link modules are 100 amino acid (aa) HA and protein-binding sequences that contain two alpha -helices and two antiparallel beta -sheets (1, 3). There are three categories of link module-containing proteins. “A” domain-type proteins contain one link module; “B” domain-type proteins contain one link module with an N- and C-terminal flanking region; and “C” domain-type proteins have an extended structure with one N-terminal V-type Ig-like domain followed by two link modules (2). The HAPLN family is a group of four C domain-type proteins that share approximately 50% aa identity (4). HAPLN1 is synthesized as a 354 aa precursor that contains a 15 aa signal sequence and a 339 aa mature region (4 - 6). It contains one Ig-like domain and two 95 aa link modules (6). It is variably glycosylated with a native molecular weight between 41 - 48 kDa (7, 8). Mature human HAPLN1 is 97%, 96%, 96%, 96%, and 96% aa identical to equine, porcine, rat, mouse and bovine HAPLN1, respectively. HAPLN1 contributes to extracellular matrix stability and flexibility (9). In cartilage, HALPN1 forms a ternary complex with HA and aggrecan. This creates a gel-like substance with remarkable resistance to deformation (3). In this complex, HA forms a linear backbone with perpendicularly attached aggrecan and HAPLN1. Aggrecan and HAPLN1 lie parallel to each other, while HA runs between the two HAPLN1 link modules (2, 3, 10). The Ig domain of HAPLN1 binds to aggrecan, while the two link modules of HAPLN1 bind to HA. Although HA and aggrecan will associate, the tendency is towards dissociation (2, 3, 8). HAPLN1 provides a stabilizing influence on HA-aggrecan associations, thus creating a long-lived ternary functional complex.

  1. Day, A.J. and G.D. Prestwich (2002) J. Biol. Chem. 277:4585.
  2. Seyfried, N.T. et al. (2005) J. Biol. Chem. 280:5435.
  3. Matsumoto, K. et al. (2003) J. Biol. Chem. 278:41205.
  4. Spicer, A.P. et al. (2003) J. Biol. Chem. 278:21083.
  5. Dudhia, J. and T.E. Hardingham (1990) Nucleic Acids Res. 18:1292.
  6. Osborne-Lawrence, S.L. et al. (1990) Genomics 8:562.
  7. Roughley, P.J. et al. (1982) J. Biol. Chem. 257:11908.
  8. Shi, S. et al. (2004) J. Biol. Chem. 279:12060.
  9. Binette, F. et al. (1994) J. Biol. Chem. 269:19116.
  10. Perkins, S.J. et al. (1992) Biochem. J. 285:263.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for HAPLN1 Antibody (MAB2608)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Western Blot.


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Review for HAPLN1 Antibody (MAB2608) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using MAB2608:
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ELISA
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
ELISA HAPLN1 MAB2608
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5
reviewed by:
Verified Customer
ELISA Human 12/09/2015
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Summary

ApplicationELISA
Sample TestedMedia from cell lines
SpeciesHuman
LotWSX0114031

Blocking

Blocking Details2 hours at room temperature with Blocking buffer from ebiosciences

Primary Anitbody

Dilution RatioMAB2608 - 1:1000 in blocking buffer , incubated 100uL per well at room temperature for 1 hour

Secondary Antibody

Secondary Descriptiongoat anti mouse
Secondary Manufacturer Cat#sc2005
Secondary Concentration1:1000 in blocking buffer , incubated 100uL per well at room temperature for 1 hour

Details

Detection Notes1x TMB - 100uL per well. reaction was stopped with 2N H2SO4
ELISA Plate DetailsCoating antibody -Rabbit polyclonal antibody from Acris Antibodies diluted 1:500 in PBS, 50uL per well overnight at 4 deg C
Wash Description5x 1 minute washes each time with PBS with 0.1% Tween-20

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Verified Customer
12/09/2015
Application: ELISA
Species: Human

Bioinformatics

Gene Symbol HAPLN1
Uniprot