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Tenascin R Antibody

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Tenascin R was detected in immersion fixed paraffin-embedded sections of human brainstem (medulla) using 1.7 µg/mL Goat Anti-Human Tenascin R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3865) ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Formulation Size Price

Tenascin R Antibody Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Tenascin R isoform 1
Glu34-Phe1358
Accession # Q92752
Specificity
Detects human Tenascin R in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human Tenascin C is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
TNR
Purity
Immunogen affinity purified
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL
Reviewed Applications
Read 1 Review rated 5
using
AF3865 in the following applications:

Publications
Read Publications using
AF3865 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Tenascin R Antibody

  • Janusin
  • MGC149328
  • Restrictin
  • tenascin R (restrictin, janusin)
  • Tenascin R
  • tenascin-R
  • TNR
  • TN-R

Background

Tenascin R (TNR) is an extracellular matrix glycoprotein belonging to the tenascin family of adhesion proteins (1-3). TNR is expressed in the central nervous system by oligodendrocytes and selected inhibitory interneurons. It shows highest expression during the postnatal period of active myelination and promotes neurite outgrowth and synaptic functions (1, 2). It is essential for formation of perineuronal nets, the mesh-like network of extracellular matrix (ECM) molecules that surrounds some neurons (4). The 180 kDa, 1327 amino acid (aa) form of human TNR contains a signal sequence, three heptad repeats that mediate coiled-coil trimer formation, five EGF-like repeats, nine fibronectin type III repeats (FN), and a C-terminal Ca2+-binding fibrinogen-related domain. TNR isoform 2 (160 kDa) lacks a portion of FN#6 (aa 773-862) (3). Mature human TNR isoform 1 shows 94%, 94%, 93%, 93%, and 76% aa identity with bovine, mouse, rat, canine, and chicken TNR, respectively. Experiments using recombinant TNR fragments indicate that EGF-like domains are counteradhesive for neurons and microglia and contribute to their migration (1, 5-7). This region interacts with immunoglobulin superfamily molecules including contactin, phosphacan and voltage-gated sodium channel beta subunits. However, the fibronectin domains are adhesive for the lectican family of chondroitin sulfate proteoglycans (brevican, aggrican, versican and neurocan; FN 3-5), contactin (FN 2-3) and sodium channel beta subunits (FN 6-8) (6-9). These adhesive interactions can compete with each other, but can also contribute to crosslinking of lecticans and contactin with other ECM molecules to form perineuronal nets (9, 10). Post-translational modification of TNR can differ with time and location (11). Notably, glycosylation may include GalNAc-4-SO4, O-linked sialylated glycans, “brain-type” neutral N-glycans and the HNK-1 carbohydrate epitope that is thought to be involved in regulation of synaptic plasticity (11, 12).

  1. Jones, F.S. and P.L. Jones (2000) Dev. Dyn. 218:235.
  2. Dityatev, A. and M. Schachner (2006) Cell Tissue Res. 326:647.
  3. Carnemolla, B. et al. (1996) J. Biol. Chem. 271: 8157.
  4. Weber, P. et al. (1999) J. Neurosci. 19:4245.
  5. Xiao, Z.C. et al. (1997) J. Neurosci. Res. 49:698.
  6. Xiao, Z.C. et al. (1999) J. Biol. Chem. 274:26511.
  7. Liao, H. et al. (2005) J. Biol. Chem. 280:8316.
  8. Aspberg, A. et al. (1997) Proc. Natl. Acad. Sci. USA 94:10116.
  9. Lundell, A. et al. (2004) Structure 12:1495.
  10. Zacharias, U. and U. Rauch (2006) J. Cell Sci. 119:3456.
  11. Woodworth, A. et al. (2004) J. Biol. Chem. 279:10413.
  12. Zamze, S. et al. (1999) Glycobiology 9:823.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Tenascin R Antibody (AF3865)(11)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 3 applications: IHC, IHC-Fr, Western Blot.


Filter By Application
IHC
(2)
IHC-Fr
(2)
Western Blot
(1)
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Human
(1)
Mouse
(2)
Rat
(1)
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Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using AF3865 Applications Species
José Javier Miguel-Hidalgo, Erik Hearn, Mohadetheh Moulana, Khunsa Saleem, Austin Clark, Maggie Holmes et al. Reduced length of nodes of Ranvier and altered proteoglycan immunoreactivity in prefrontal white matter in major depressive disorder and chronically stressed rats Sci Rep 2023-09-29 [PMID: 37775676]
Hiroshi Ueno, Yu Takahashi, Shinji Murakami, Kenta Wani, Tetsuji Miyazaki, Yosuke Matsumoto et al. Component-specific reduction in perineuronal nets in senescence-accelerated mouse strains IBRO Neuroscience Reports 2023-06-01 [PMID: 37388498]
M Doty, S Yun, Y Wang, M Hu, M Cassidy, B Hall, AB Kulkarni Integrative multiomic analyses of dorsal root ganglia in diabetic neuropathic pain using proteomics, phospho-proteomics, and metabolomics Scientific Reports, 2022-10-11;12(1):17012. 2022-10-11 [PMID: 36220867] (IHC, Western Blot, Human) IHC, Western Blot Human
Charly Abi-Ghanem, Deepa Jonnalagadda, Jerold Chun, Yasuyuki Kihara, Barbara Ranscht CAQK, a peptide associating with extracellular matrix components targets sites of demyelinating injuries Frontiers in Cellular Neuroscience 2022-08-22 [PMID: 36072569]
Kojiro Nojima, Haruko Miyazaki, Tetsuya Hori, Lydia Vargova, Toshitaka Oohashi Assessment of Possible Contributions of Hyaluronan and Proteoglycan Binding Link Protein 4 to Differential Perineuronal Net Formation at the Calyx of Held Frontiers in Cell and Developmental Biology 2021-09-17 [PMID: 34604231]
Andrea Hunyadi, Botond Gaál, Clara Matesz, Zoltan Meszar, Markus Morawski, Katja Reimann et al. Distribution and classification of the extracellular matrix in the olfactory bulb Brain Structure and Function 2020-01-01 [PMID: 31858237]
H Ueno, S Suemitsu, S Murakami, N Kitamura, K Wani, Y Matsumoto, M Okamoto, T Ishihara Layer-specific expression of extracellular matrix molecules in the mouse somatosensory and piriform cortices IBRO Rep, 2018-11-28;6(0):1-17. 2018-11-28 [PMID: 30582064] (IHC-Fr, Mouse) IHC-Fr Mouse
H Ueno, S Suemitsu, M Okamoto, Y Matsumoto, T Ishihara Sensory experience-dependent formation of perineuronal nets and expression of Cat-315 immunoreactive components in the mouse somatosensory cortex Neuroscience, 2017-05-08;0(0):. 2017-05-08 [PMID: 28495333] (IHC-Fr, Mouse) IHC-Fr Mouse
Aman P. Mann, Pablo Scodeller, Sazid Hussain, Jinmyoung Joo, Ester Kwon, Gary B. Braun et al. A peptide for targeted, systemic delivery of imaging and therapeutic compounds into acute brain injuries Nature Communications 2016-06-28 [PMID: 27351915]
Stephanie Dauth, Thomas Grevesse, Harry Pantazopoulos, Patrick H. Campbell, Ben M. Maoz, Sabina Berretta et al. Extracellular matrix protein expression is brain region dependent Journal of Comparative Neurology 2016-05-01 [PMID: 26780384]
Show All 11 Publications.

Review for Tenascin R Antibody (AF3865) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Other.

Reviews using AF3865:
Filter by Applications
IHC-P
(1)
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Other
(1)
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Images Ratings Applications Species Date Details
  5
reviewed by:
Verified Customer
IHC-P Other 01/08/2015
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedSee PMID 24269935
SpeciesOther

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Verified Customer
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Application: IHC-P
Species: Other

Bioinformatics

Gene Symbol TNR
Uniprot