Tenascin R Antibody

Tenascin R was detected in immersion fixed paraffin-embedded sections of human brainstem (medulla) using 1.7 µg/mL Goat Anti-Human Tenascin R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3865) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Tenascin R Antibody Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Tenascin R isoform 1
  Glu34-Phe1358
  Accession # Q92752
• Specificity: Detects human Tenascin R in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human Tenascin C is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TNR
• Purity: Immunogen affinity purified
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Tenascin R Antibody

• Janusin
• MGC149328
• Restrictin
• tenascin R (restrictin, janusin)
• Tenascin R
• tenascin-R
• TNR
• TN-R

Background

Tenascin R (TNR) is an extracellular matrix glycoprotein belonging to the tenascin family of adhesion proteins (1-3). TNR is expressed in the central nervous system by oligodendrocytes and selected inhibitory interneurons. It shows highest expression during the postnatal period of active myelination and promotes neurite outgrowth and synaptic functions (1, 2). It is essential for formation of perineuronal nets, the mesh-like network of extracellular matrix (ECM) molecules that surrounds some neurons (4). The 180 kDa, 1327 amino acid (aa) form of human TNR contains a signal sequence, three heptad repeats that mediate coiled-coil trimer formation, five EGF-like repeats, nine fibronectin type III repeats (FN), and a C-terminal Ca²⁺-binding fibrinogen-related domain. TNR isoform 2 (160 kDa) lacks a portion of FN#6 (aa 773-862) (3). Mature human TNR isoform 1 shows 94%, 94%, 93%, 93%, and 76% aa identity with bovine, mouse, rat, canine, and chicken TNR, respectively. Experiments using recombinant TNR fragments indicate that EGF-like domains are counteradhesive for neurons and microglia and contribute to their migration (1, 5-7). This region interacts with immunoglobulin superfamily molecules including contactin, phosphacan and voltage-gated sodium channel beta subunits. However, the fibronectin domains are adhesive for the lectican family of chondroitin sulfate proteoglycans (brevican, aggrican, versican and neurocan; FN 3-5), contactin (FN 2-3) and sodium channel beta subunits (FN 6-8) (6-9). These adhesive interactions can compete with each other, but can also contribute to crosslinking of lecticans and contactin with other ECM molecules to form perineuronal nets (9, 10). Post-translational modification of TNR can differ with time and location (11). Notably, glycosylation may include GalNAc-4-SO₄, O-linked sialylated glycans, “brain-type” neutral N-glycans and the HNK-1 carbohydrate epitope that is thought to be involved in regulation of synaptic plasticity (11, 12).

1. Jones, F.S. and P.L. Jones (2000) Dev. Dyn. 218:235.
2. Dityatev, A. and M. Schachner (2006) Cell Tissue Res. 326:647.
3. Carnemolla, B. et al. (1996) J. Biol. Chem. 271: 8157.
4. Weber, P. et al. (1999) J. Neurosci. 19:4245.
5. Xiao, Z.C. et al. (1997) J. Neurosci. Res. 49:698.
6. Xiao, Z.C. et al. (1999) J. Biol. Chem. 274:26511.
7. Liao, H. et al. (2005) J. Biol. Chem. 280:8316.
8. Aspberg, A. et al. (1997) Proc. Natl. Acad. Sci. USA 94:10116.
9. Lundell, A. et al. (2004) Structure 12:1495.
10. Zacharias, U. and U. Rauch (2006) J. Cell Sci. 119:3456.
11. Woodworth, A. et al. (2004) J. Biol. Chem. 279:10413.
12. Zamze, S. et al. (1999) Glycobiology 9:823.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Tenascin R Antibody (AF3865)(4)

Publications using AF3865

• M Doty, S Yun, Y Wang, M Hu, M Cassidy, B Hall, AB Kulkarni Integrative multiomic analyses of dorsal root ganglia in diabetic neuropathic pain using proteomics, phospho-proteomics, and metabolomics Scientific Reports, 2022;12(1):17012. 2022 [PMID: 36220867] (Western Blot, Human)
• H Ueno, S Suemitsu, S Murakami, N Kitamura, K Wani, Y Matsumoto, M Okamoto, T Ishihara Layer-specific expression of extracellular matrix molecules in the mouse somatosensory and piriform cortices IBRO Rep, 2019;6(0):1-17. 2019 [PMID: 30582064] (IHC-Fr, Mouse)
• H Ueno, S Suemitsu, M Okamoto, Y Matsumoto, T Ishihara Sensory experience-dependent formation of perineuronal nets and expression of Cat-315 immunoreactive components in the mouse somatosensory cortex Neuroscience, 2017;0(0):. 2017 [PMID: 28495333] (IHC-Fr, Mouse)
• Kecskes S, Gaal B, Racz E, Birinyi A, Hunyadi A, Matesz C Extracellular matrix molecules exhibit unique expression pattern in the climbing fiber-generating precerebellar nucleus, the inferior olive. Neuroscience, 2015;284(0):412-21. 2015 [PMID: 25445196] (IHC, Rat)

Review for Tenascin R Antibody (AF3865) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Other.

reviewed by: Anonymous - IHC-P Other 01/08/2015

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: See PMID 24269935
• Species: Other

Bioinformatics

• Gene Symbol: TNR
• Uniprot:
  • Q92752()