HADH Antibody (D10-E7)

Western Blot: HADH Antibody (D10-E7) [NBP2-66880] - Analysis on different tissue lysates using anti- HADHSC mouse mAb.

Immunocytochemistry/Immunofluorescence: HADH Antibody (D10-E7) [NBP2-66880] - Staining HADHSC in 293 cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Product Discontinued

HADH Antibody (D10-E7) Summary

• Immunogen: Synthetic peptide within Human HADH aa 265-314 / 314. (SwissProt: Q16836 Human)
• Localization: Mitochondrion matrix
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: HADH
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:500
  • Immunohistochemistry-Paraffin
  • Western Blot 1:1000-1:2000

Reactivity Notes

Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-questions.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.2% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 2 mg/ml
• Purity: Protein A purified

Alternate Names for HADH Antibody (D10-E7)

• EC 1.1.1
• EC 1.1.1.35
• HADH1
• HADHSChydroxyacyl-Coenzyme A dehydrogenase
• HCDH
• hydroxyacyl-CoA dehydrogenase
• L-3-hydroxyacyl-Coenzyme A dehydrogenase, short chain
• Medium and short-chain L-3-hydroxyacyl-coenzyme A dehydrogenase
• MGC8392
• mitochondrial
• MSCHAD
• SCHADHHF4

Background

HADH is a member of the 3-hydroxyacyl-CoA dehydrogenase gene family. The encoded protein functions in the mitochondrial matrix to catalyze the oxidation of straight-chain 3-hydroxyacyl-CoAs as part of the beta-oxidation pathway. Its enzymatic activity is highest with medium-chain-length fatty acids. Mutations in this gene cause one form of familial hyperinsulinemic hypoglycemia. The human genome contains a related pseudogene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HADH