GW182 Antibody

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Immunoprecipitation: GW182 Antibody [NBP1-28702] - Immunoprecipitation on mouse brain samples - (dissected hippocampus). The recommended dilution is 1:100.
Immunoprecipitation: GW182 Antibody [NBP1-28702] - Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-GW182 antibody NBP1-28702 used for IP at 10 ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications IP, WB (-)
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

GW182 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 1225 and 1275 of human glycine-tryptophan protein of 182 kDa (GW182) using the numbering given in entry NP_055309.2 (GeneID 27327).
Marker
P/GW Body Marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TNRC6A
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunoprecipitation 10 ug/mg lysate
Theoretical MW
210 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for GW182 Antibody

  • CAG repeat protein 26
  • CAGH26FLJ22043
  • DKFZp666E117
  • EMSY interactor protein
  • Glycine-tryptophan protein of 182 kDa
  • GW182 autoantigen
  • GW182GW1
  • KIAA1460EDIE
  • MGC75384
  • Protein GW1
  • TNRC6
  • trinucleotide repeat containing 6
  • trinucleotide repeat containing 6A
  • trinucleotide repeat-containing gene 6A protein

Background

GW182 (glycine-tryptophan protein of 182 kDa) is a member of the trinucleotide repeat containing 6 protein family. It functions in post-transcriptional gene silencing through the RNA interference (RNAi) and microRNA pathways. GW182 has been found to associate with messenger RNAs and Argonaute proteins in cytoplasmic bodies known as GW-bodies or P-bodies. Inhibition of GW182 expression delocalizes other GW-body proteins and impairs RNAi and microRNA-induced gene silencing.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol TNRC6A
Entrez
Uniprot