![Immunoprecipitation: GW182 Antibody [NBP1-28702] - Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-GW182 antibody NBP1-28702 used for IP at 10 ug/mg lysate. GW182 was also immunoprecipitated by rabbit anti-GW182 antibody NBP1-28751, which recognizes an upstream epitope. For blotting immunoprecipitated GW182, NBP1-28751 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/GW182-Antibody-Immunoprecipitation-NBP1-28702-img0003.jpg "Immunoprecipitation: GW182 Antibody [NBP1-28702] - Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-GW182 antibody NBP1-28702 used for IP at 10 ug/mg lysate. GW182 was also immunoprecipitated by rabbit anti-GW182 antibody NBP1-28751, which recognizes an upstream epitope. For blotting immunoprecipitated GW182, NBP1-28751 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | IP, WB (-) |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1.0 mg/ml |
| Description | Novus Biologicals Rabbit GW182 Antibody - BSA Free (NBP1-28702) is a polyclonal antibody validated for use in IP. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Immunogen | The immunogen recognized by this antibody maps to a region between residue 1225 and 1275 of human glycine-tryptophan protein of 182 kDa (GW182) using the numbering given in entry NP_055309.2 (GeneID 27327). |
| Marker | P/GW Body Marker |
| Predicted Species | Mouse (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | TNRC6A |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Western Blot Not recommended. |
| Theoretical MW | 210 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C. Do not freeze. |
| Buffer | Tris-Citrate/Phosphate (pH 7.0 - 8.0) |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
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![Immunoprecipitation: GW182 Antibody [NBP1-28702] - Immunoprecipitation on mouse brain samples - (dissected hippocampus). The recommended dilution is 1:100.](http://images.novusbio.com/fullsize/GW182-Antibody-Immunoprecipitation-NBP1-28702-img0002.jpg "Immunoprecipitation: GW182 Antibody [NBP1-28702] - Immunoprecipitation on mouse brain samples - (dissected hippocampus). The recommended dilution is 1:100.")
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