Recombinant Human Guanine deaminase His Protein

SDS-Page: Recombinant Human Guanine deaminase Protein [NBP2-52618]

• Reactivity: Hu
• Applications: PAGE, Enzyme Activity
• Concentration: 1 mg/ml

Recombinant Human Guanine deaminase His Protein Summary

• Description: A bioactive recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-454 of Human Guanine deaminase
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMCAAQMP PLAHIFRGTF VHSTWTCPME VLRDHLLGVS DSGKIVFLEE ASQQEKLAKE WCFKPCEIRE LSHHEFFMPG LVDTHIHASQ YSFAGSSIDL PLLEWLTKYT FPAEHRFQNI DFAEEVYTRV VRRTLKNGTT TACYFATIHT DSSLLLADIT DKFGQRAFVG KVCMDLNDTF PEYKETTEES IKETERFVSE MLQKNYSRVK PIVTPRFSLS CSETLMGELG NIAKTRDLHI QSHISENRDE VEAVKNLYPS YKNYTSVYDK NNLLTNKTVM AHGCYLSAEE LNVFHERGAS IAHCPNSNLS LSSGFLNVLE VLKHEVKIGL GTDVAGGYSY SMLDAIRRAV MVSNILLINK VNEKSLTLKE VFRLATLGGS QALGLDGEIG NFEVGKEFDA ILINPKASDS PIDLFYGDFF GDISEAVIQK FLYLGDDRNI EEVYVGGKQV VPFSSSV

• Details of Functionality: Specific activity is >2,000 pmol/min/ug, and is defined as the amount of enzyme that convert guanine to xanthine per minute at pH 8.0 at 37C.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: GDA
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • Enzyme Activity
  • SDS-Page
• Theoretical MW: 53 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH8.0), 0.15 M NaCl, 10% glycerol, 1 mM DTT
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human Guanine deaminase His Protein

• CYPIN
• EC 3.5.4.3
• GAH
• GUANASE
• Guanine aminase
• Guanine aminohydrolase
• guanine deaminase
• KIAA1258
• MGC9982
• NEDASIN
• p51-nedasin

Background

GDA is an enzyme responsible for the hydrolytic deamination of guanine. Studies in rat ortholog suggest this gene plays a role in microtubule assembly. Multiple transcript variants encoding different isoforms have been found for this gene. Recombinant human GDA protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: GDA
• Uniprot:
  • NP_004284(Human)