Recombinant Human Guanine deaminase Protein

Recombinant Human Guanine deaminase Protein [NBP2-49692]

• Reactivity: Hu
• Applications: PAGE, Enzyme Activity
• Concentration: 1 mg/ml

Recombinant Human Guanine deaminase Protein Summary

• Description: An un-tagged recombinant protein corresponding to the amino acids 1-454 of Human Guanine deaminase
  
  

  Source: E.coli

  Amino Acid Sequence: MCAAQMPPLA HIFRGTFVHS TWTCPMEVLR DHLLGVSDSG KIVFLEEASQ QEKLAKEWCF KPCEIRELSH HEFFMPGLVD THIHASQYSF AGSSIDLPLL EWLTKYTFPA EHRFQNIDFA EEVYTRVVRR TLKNGTTTAC YFATIHTDSS LLLADITDKF GQRAFVGKVC MDLNDTFPEY KETTEESIKE TERFVSEMLQ KNYSRVKPIV TPRFSLSCSE TLMGELGNIA KTRDLHIQSH ISENRDEVEA VKNLYPSYKN YTSVYDKNNL LTNKTVMAHG CYLSAEELNV FHERGASIAH CPNSNLSLSS GFLNVLEVLK HEVKIGLGTD VAGGYSYSML DAIRRAVMVS NILLINKVNE KSLTLKEVFR LATLGGSQAL GLDGEIGNFE VGKEFDAILI NPKASDSPID LFYGDFFGDI SEAVIQKFLY LGDDRNIEEV YVGGKQVVPF SSSV

• Details of Functionality: Specific activity is > 2,000 pmol/min/ug, and is defined as the amount of enzyme that convert guanine to xanthine per minute at pH 8.0 at 37C.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: GDA
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • Enzyme Activity
  • SDS-Page
• Theoretical MW: 51 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 10% glycerol, 1 mM DTT
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human Guanine deaminase Protein

• CYPIN
• EC 3.5.4.3
• GAH
• GUANASE
• Guanine aminase
• Guanine aminohydrolase
• guanine deaminase
• KIAA1258
• MGC9982
• NEDASIN
• p51-nedasin

Background

GDA, also known as guanine deaminase isoform b, catalyzes the hydrolytic deamination of guanine, producing xanthine and ammonia. Studies in rat ortholog suggest this gene plays a role in microtubule assembly. Multiple transcript variants encoding different isoforms have been found for this gene. Recombinant human GDA, was expressed in E.coli and purified by conventional chromatography techniques. Produced in E. coli

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Publications for Guanine deaminase Biologically Active Protein (NBP2-49692)(1)

Publication using NBP2-49692

• Ramirez-Celis A, Becker M, Nuño M, et al. Risk assessment analysis for maternal autoantibody-related autism (MAR-ASD): a subtype of autism Molecular psychiatry 2021-01-22 [PMID: 33483694]

Bioinformatics

• Gene Symbol: GDA